Abstract
The objective of this paper is to give a brief overview of our laboratory’s present activities utilizing a culture system which supports the longterm growth of cells restricted to the B-lymphocyte lineage. These studies are aimed at understanding the fundamental mechanisms involved in the normal development of B-lymphocytes. This in vitro system can overcome some difficulties associated with heterogeneous cell populations and interpretations of studies utilizing immortalized B cell lymphoma lines.
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References
Amsbaugh DF, Hansen CT, Prescott B, Stashak PW, Barthold DR, Baker PJ (1972) Genetic control of the antibody response to type III pneumococcal polysaccharide in mice. I. Evidence that an X-linked gene plays a decisive role in determining responsiveness. J Exp Med 136: 931–949.
Dexter TM, Allen TD, Lajtha LG (1977) Conditions controlling the in vitro proliferation of hemopoietic stem cells in vitro. J Cell Physiol 91: 335–340.
Jones-Villeneuve EV, Phillips RA (1980) Potentials for lymphoid differentiation by cells from longterm cultures of bone marrow. Exp Hemat 8: 65–76.
Kincade PW, Lee G, Watanabe T, Sun L, Scheid MP (1981) Antigens displayed on murine B-lymphocyte precursors. J Immunol 127: 2262–2268.
Kincade PW (1977) Defective colony formation by B lymphocytes from CBA/N and C3H/HeJ mice. J Exp Med 145: 249–263.
Kurland JI, Kincade PW, Moore MAS (1977) Regulation of B lymphocyte clonal proliferation by stimulatory and inhibitory macrophage derived factors. J Exp Med 146: 1420–1433.
Kurland JI, Bockman R, Broxmeyer HE, Moore MAS (1978) Limitation of excessive myelopoiesis by the intrinsic modulation of macrophage derived prostaglandin E. Science 199: 552–555.
Kurland JI, Meyers PA, Moore MAS (1980) Synthesis and release of erythroid colony- and burst-promoting activities by purified populations of murine peritoneal macrophages. J Exp Med 151: 839–852.
Metcalf D, Wilson JW, Shortman K, Miller JFAP, Stocker J (1976) The nature of cells generating B lymphocyte colonies in vitro. J Cell Physiol 88: 107–116.
Mosier DE, Zitron M, Mond J, Ahmed A, Scher I, Paul WE (1977) Surface immunoglobulin D as a functional receptor for a subclass of B-lymphocytes. Immunol Rev 37: 89–104.
Nottenberg C, Weissman IL (1981) Cu gene rearrangement of mouse immunoglobulin genes in normal B cells on both the expressed and non-expressed chromosomes. Proc Natl Acad Sci 78: 484–488.
Scher I, Steinberg AD, Bernig AK, Paul WE (1974) X-linked B-lymphocyte immune defect in CBA/N mice. II. Studies of the mechanism underlying the immune defect. J Exp Med 142: 637–649.
Schrader JW, Schrader S (1978) In vitro studies on lymphocyte differentiation. I. Longterm in vitro culture of cells giving rise to functional lymphocytes in irradiated mice. J Exp Med 148: 823–828.
Till JE, McCulloch EA (1961) A direct measurement of the radiation sensitivity of normal bone marrow cells. Radiat Res 14: 213–222.
Whitlock CA, Witte ON (1982) Longterm culture of B-lymphocytes and their precursors from murine bone marrow. Proc Natl Acad Sci 79: 3608–3612.
Whitlock CA, Zeigler SF, Treiman LJ, Stafford JI, Witte ON (1983) Differentiation of cloned populations of immature B cells after transformation with Abelson murine leukemia virus. Cell 32: 903–911.
Whitlock CA, Robertson D, Witte ON (1984) Murine B cell lymphopoiesis in lonyterm culture. J Immunol Meth (in press).
Witte ON, Whitlock CA, Robertson D, Kurland JI, Swansen SK., Treiman JL, Denis K, Stafford, JI (1984) Growth regulation of murine B-lymphoid cells with and without the v-abl oncogene. In: Levine A, Vandewoude G (eds) The Cancer Cell. Cold Spring Harbor Conferences on Cell Proliferation and Cancer Vol II, Cold Spring Harbor, New York.
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© 1984 Springer-Verlag Berlin · Heidelberg
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Kurland, J.I., Witte, O.N. (1984). Development of Cells of the B-Lymphocyte Lineage in Longterm Culture. In: Potter, M., Melchers, F., Weigert, M. (eds) Oncogenes in B-Cell Neoplasia. Current Topics in Microbiology and Immunology, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69860-6_37
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DOI: https://doi.org/10.1007/978-3-642-69860-6_37
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-69862-0
Online ISBN: 978-3-642-69860-6
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