Summary
Using a variety of myc-specific antibodies against bacterially expressed viral and human cellular myc genes and against synthetic peptides, we have characterized viral and cellular myc-gene products and obtained the following results:
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(1)
Immunofluorescence analysis of MC29-transformed fibroblasts sequentially treated with detergent, nucleases and salt indicates that part of the p11Ogag-myc remains insoluble suggesting its association with the so-called nuclear matrix.
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(2)
Immune-affinity purified p11Ogag-myc protein inhibits transcription of the Adenovirus 2 major late promoter (Ad2 MLP) two- to fourfold if added to in vitro transcription systems.
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(3)
The c-myc gene product expressed in avian lymphoma (RP9) cells was identified as a protein of 55,000 molecular weight, p55c-myc, similar to the avian viral p55v-myc protein of OK10 cells.
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(4)
Myc-specific antibodies gave rise to nuclear fluorescence in HeLa cells and precipitated two proteins of about 62,000 and 49,000 molecular weights which were competed out by the appropriate antigens.
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(5)
Mitogen-stimulated lymphocytes exhibit two novel proteins of similar size detected by myc-specific antibodies.
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References
Abrams HD, Rohrschneider LR, Eisenman RN (1982) Nuclear localization of the putative protein of avian myelocytomatosis virus. Cell 29: 427–439
Alitalo K, Ramsay G, Bishop JM, Ohlsson-Pfeiffer S, Colby WW, Levinson AD (1983) Identification of nuclear proteins encoded by viral and cellular myc oncogenes. Nature 306: 274–277
Bunte T, Greiser-Wilke I, Donner P, Moelling K (1982) Association of gag-myc proteins from avian myelocytomatosis virus wild-type and mutants with chromatin. EMBO J 1: 919–927
Bunte T, Greiser-Wilke I, Moelling K (1983) The transforming protein of the MC29-related virus CMII is a nuclear DNA-binding protein whereas MH2 codes for a cytoplasmic RNA-DNA binding polyprotein. EMBO J 2: 1087–1092
Ciejek, E.M., Tsai MJ, O’Malley BW (1983) Actively transcribed genes are associated with the nuclear matrix. Nature 306: 607–609
Davison BL, Egly JM, Mulvihill ER, Chambon P (1983) Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences. Nature 304: 680–686
Donner P, Bunte T, Greiser-Wilke I, Moelling K (1983) Decreased DNA- binding ability of purified transformation-specific proteins from deletion mutants of the acute avian leukemia virus MC29. Proc Natl Acad Sci USA 80: 2861–2865
Donner P, Greiser-Wilke I, Moelling K (1982) Nuclear localization and DNA binding of the transforming gene product of avian myelocytomatosis virus. Nature 296: 262–266
Eisenman R, Hann S, Abrams H, Tachibana C, Rohrschneider L (1984) The myc oncogene encodes a class of nuclear proteins: possible association with the nuclear matrix. In: Levine A, van de Woude G (eds) Cell proliferation and cancer, vol 11. Cold Spring Harbor Press (in press)
Gazin C, Dupont de Dinechin S, Hampe A, Masson J-M, Martin P, Stehelin D, Galibert F (1984) Nucleotide sequence of the human c-myc locus: provocative open reading frame within the first exon. EMBO J 3: 383–387
Giallongo A, Appella E, Riccardi R, Rovera G, Croce CM (1983) Identification of the c-myc oncogene product in normal and malignant B cells. Science 222: 430–432
Greiser-Wilke I, Owada MK, Moelling K (1981) Isolation of monoclonal antibodies against the avian oncornaviral protein p19. J Virol 39: 325–329
Hann SR, Abrams H, Rohrschneider LR, Eisenman RN (1983) Proteins encoded by v-myc and c-myc oncogenes: identification and localization in acute leukemia virus transformants and bursal lymphoma cell lines. Cell 34: 789–798
Hayward WS, Neel BC, Astrin SM (1981) Activation of a cellular one gene by promoter insertion in ALV-induced lymphoid leukosis. Nature 290: 475–480
Hen R, Sassone-Corsi P, Corden J, Gaub MP, Chambon P (1982) Sequences upstream from the T-A-T-A- box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter. Proc Natl Acad Sci USA 79: 7132–7136
Kelly K, Cochran BH, Stiles CD, Leder P (1983) Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell 35: 603–610
Moelling K (1984) Fusion proteins in retroviral transformation. In: Weinhouse S, Klein G (eds) Advances in cancer research, vol 42. Academic Press, New York (in press)
Moelling K, Bunte T, Greiser-Wilke I, Donner P, Pfaff E (1984) Properties of avian viral transforming proteins gag-myc, myc and gag-mil. In: Levine A, van de Woude G (eds) Cell proliferation and cancer, vol 11. Cold Spring Harbor Press (in press)
Newmark P (1983) The rasmatazz of cancer genes. Nature 305: 407–471
Staufenbiel M, Deppert W (1983) Different structural systems of the nucleus are targets for SV40 large T antigen. Cell 33: 173–181
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Moelling, K. et al. (1984). Properties of the myc-Gene Product: Nuclear Association, Inhibition of Transcription and Activation in Stimulated Lymphocytes. In: Potter, M., Melchers, F., Weigert, M. (eds) Oncogenes in B-Cell Neoplasia. Current Topics in Microbiology and Immunology, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69860-6_34
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DOI: https://doi.org/10.1007/978-3-642-69860-6_34
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