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Immuno Affinity Purified Interleukin 2 (IL2) Induces B Cell Growth

  • J. J. Mond
  • C. Thompson
  • M. Schaefer
  • F. D. Finkelman
  • R. Robb
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 113)

Abstract

With the recent availability of immuno-affinity purified IL2 (Robb 1983) we attempted to investigate whether B cell growth can be directly influenced by this lymphokine. In view of our recent findings that large “activated” B cells are more receptive than small “resting” B cells to the growth stimulating effects of EL4 derived B cell growth factor (Mond, 1984) we undertook to study the effects of IL2 on size separated populations of B cells. B cells were prepared by a modification of the method of Leibson (1981) and then separated by counterflow Centrifugation into five size dependent fractions (Thompson, 1983). The average size of the separated B cells in the smallest sized fraction (fraction 1) was 115 µ and each successive fraction had a progressively larger mean cell volume with the largest sized (fractions) being 168 µ3. Less than 2% Thy 1.2+ cells were present in any of the fractions. The immuno-affinity purified IL2 used in these experiments was prepared by passing supernatant coupled with a murine monoclonal antibody reactive with human IL2. Bound IL2 was recovered by elution of the column with 1.5% acetic acid (Robb, et al 1983).

Keywords

Murine Monoclonal Antibody Recent Availability Growth Stimulate Effect Bridge Road Murine Spleen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Leibson H, Marrack P, Kappler J (1981) B cell helper factors. II. Synergy among three helper factors in the response of T cell and macrophage depleted B cells. J Immunol 139: 1398Google Scholar
  2. Malek R, Robb RJ, Schevach EM (1983) Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex. Proc Natl Acad Sei USA 80: 5694–5698CrossRefGoogle Scholar
  3. Mond JJ, Thompson CB, Schaefer ME, Farrar J, Finkelman FD (1984) Responsiveness of size separated Lyb5 B cells from immune defective (CBA/N x DBA/2) mice to a chromatographically purified B cell growth factor. J Immunol (Submitted for publication)Google Scholar
  4. Robb RJ, Kutny RM, Chowdry, V (1983) Purification and partial sequence analysis of human T-cell growth factor. Proc Natl Acad Sei USA 80: 5990–5994CrossRefGoogle Scholar
  5. Thompson CB, Ryan JJ, Sieckmann G, Finkelman FD, Mond JJ, Scher I (1983) A method for size separation of murine spleen cells using counterflow centrifugation. J Immunol Methods 63: 299–307PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1984

Authors and Affiliations

  • J. J. Mond
    • 1
  • C. Thompson
    • 2
  • M. Schaefer
    • 1
  • F. D. Finkelman
    • 1
  • R. Robb
    • 3
  1. 1.Department of MedicineUniformed Services University of the Health SciencesBethesdaUSA
  2. 2.Naval Medical Research InstituteBethesdaUSA
  3. 3.Central Research & Development, E.I. duPont deNemours & CompanyGlenolden LaboratoryGlenoldenUSA

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