Immuno Affinity Purified Interleukin 2 (IL2) Induces B Cell Growth

  • J. J. Mond
  • C. Thompson
  • M. Schaefer
  • F. D. Finkelman
  • R. Robb
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 113)

Abstract

With the recent availability of immuno-affinity purified IL2 (Robb 1983) we attempted to investigate whether B cell growth can be directly influenced by this lymphokine. In view of our recent findings that large “activated” B cells are more receptive than small “resting” B cells to the growth stimulating effects of EL4 derived B cell growth factor (Mond, 1984) we undertook to study the effects of IL2 on size separated populations of B cells. B cells were prepared by a modification of the method of Leibson (1981) and then separated by counterflow Centrifugation into five size dependent fractions (Thompson, 1983). The average size of the separated B cells in the smallest sized fraction (fraction 1) was 115 µ and each successive fraction had a progressively larger mean cell volume with the largest sized (fractions) being 168 µ3. Less than 2% Thy 1.2+ cells were present in any of the fractions. The immuno-affinity purified IL2 used in these experiments was prepared by passing supernatant coupled with a murine monoclonal antibody reactive with human IL2. Bound IL2 was recovered by elution of the column with 1.5% acetic acid (Robb, et al 1983).

Keywords

Thymidine Concanavalin 

References

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1984

Authors and Affiliations

  • J. J. Mond
    • 1
  • C. Thompson
    • 2
  • M. Schaefer
    • 1
  • F. D. Finkelman
    • 1
  • R. Robb
    • 3
  1. 1.Department of MedicineUniformed Services University of the Health SciencesBethesdaUSA
  2. 2.Naval Medical Research InstituteBethesdaUSA
  3. 3.Central Research & Development, E.I. duPont deNemours & CompanyGlenolden LaboratoryGlenoldenUSA

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