Abstract
With the recent availability of immuno-affinity purified IL2 (Robb 1983) we attempted to investigate whether B cell growth can be directly influenced by this lymphokine. In view of our recent findings that large “activated” B cells are more receptive than small “resting” B cells to the growth stimulating effects of EL4 derived B cell growth factor (Mond, 1984) we undertook to study the effects of IL2 on size separated populations of B cells. B cells were prepared by a modification of the method of Leibson (1981) and then separated by counterflow Centrifugation into five size dependent fractions (Thompson, 1983). The average size of the separated B cells in the smallest sized fraction (fraction 1) was 115 µ and each successive fraction had a progressively larger mean cell volume with the largest sized (fractions) being 168 µ3. Less than 2% Thy 1.2+ cells were present in any of the fractions. The immuno-affinity purified IL2 used in these experiments was prepared by passing supernatant coupled with a murine monoclonal antibody reactive with human IL2. Bound IL2 was recovered by elution of the column with 1.5% acetic acid (Robb, et al 1983).
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References
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Thompson CB, Ryan JJ, Sieckmann G, Finkelman FD, Mond JJ, Scher I (1983) A method for size separation of murine spleen cells using counterflow centrifugation. J Immunol Methods 63: 299–307
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© 1984 Springer-Verlag Berlin · Heidelberg
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Mond, J.J., Thompson, C., Schaefer, M., Finkelman, F.D., Robb, R. (1984). Immuno Affinity Purified Interleukin 2 (IL2) Induces B Cell Growth. In: Potter, M., Melchers, F., Weigert, M. (eds) Oncogenes in B-Cell Neoplasia. Current Topics in Microbiology and Immunology, vol 113. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69860-6_19
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DOI: https://doi.org/10.1007/978-3-642-69860-6_19
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