Bestimmung von Zink in Thrombocyten mit INAA und AAS

  • J. Kiem
  • H. Weese
  • L. E. Feinendegen
Conference paper

Summary

To clarify discrepancies in the literature we determined the concentration of zinc in normal human platelets using instrumental neutron activation analysis (INAA, n = 26) and atomic absorption spectrophotometry (AAS, n = 5 × 6). We detected the following sources of error: zinc contamination, erroneous platelet wet weight, errors when using INAA. Additionally, we determined the concentrations of calcium and magnesium in the AAS-samples (n = 5 × 6). We observed that after elimination of the sources of error the normal ranges (mean ± 2 SD) for the elements zinc, calcium and magnesium are far closer than hitherto believed. Based on platelet wet weight corrected for plasma, they are: Zn = 26.5–32.3 µg/g; Ca = 567–693 µg/g, Mg = 178–218µg/g.

Keywords

Zinc Magnesium Spectrophotometry INAA Sten 

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Literatur

  1. 1.
    Aktulga A, Ulutin ON (1975) Platelets. Excerpta Medica, Amsterdam, S 185–191Google Scholar
  2. 2.
    Foley B, Johnson SA, Hackley B, Smith JC Jr, Halsted JA (1968) Proc Soc Exp Biol Med 128:265–269Google Scholar
  3. 3.
    Iyengar GV, Kollmer WE, Bowen HJM (1978) The elemental composition of human tissues and body fluids. Verlag Chemie, Weinheim New YorkGoogle Scholar
  4. 4.
    Kasperek K, Iyengar GV, Kiem J, Borberg H, Feinendegen LE (1979) Clin Chem 25:711–715Google Scholar
  5. 5.
    Kiem J, Borberg H, Iyengar GV, Kasperek K, Siegers M, Feinendegen LE, Gross R (1979) Clin Chem 25:705–710Google Scholar
  6. 6.
    Kiem J, Iyengar GV, Kasperek K, Lombeck I, Wang YX, Feinendegen LE (1981) Mineral elements ’80, part I. Academy of Finland, Helsinki, S 253–257Google Scholar
  7. 7.
    Kiem J, Feinendegen LE (1982) Vortragskurzfassung der AGF-Veranstaltung „Schadstoffe in Nahrungsketten, Nov 1982, BonnGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • J. Kiem
    • 1
  • H. Weese
    • 1
  • L. E. Feinendegen
    • 1
  1. 1.Institut für MedizinKernforschungsanlage Jülich GmbHJülichBundesrepublik Deutschland

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