Specificity of the Ninth Workshop Anti Ia Alloantisera as Assessed by the Direct Binding Assay

  • N. Tanigaki
  • P. Overturf
  • L. Rendina
  • J. Shaver
  • R. Tosi
  • G. B. Ferrara
Conference paper


Antibody specificity can be easily defined when the binding of antibody to antigen is measured directly. A sensitive determination of such direct binding is possible by the use of radiolabeled antigen. This is also the case for alloantigens [1, 2]. We have been using 125I-labeled la antigens for specificity assay of the alloantisera and more recently of the monoconal antibodies. The assay method (we call it the direct binding assay) first allows reaction of a test alloantiserum or a monoclonal antibody with a radioiodinated la preparation, and then measures the extent of binding by the double-antibody technique [2]. When a panel of 125I-labeled la preparations of different specificities is used, the major or putative antibody specificity can be deduced from the reaction pattern. This assay has been found particularly useful in the search for antibodies that can be used for la typing by the binding inhibition assay [2, 3] and also for immunospecific isolation of la molecules carrying different specificities [4].


Celiac Disease Reaction Pattern North Atlantic Treaty Organization Positive Binding Direct Binding Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Tanigaki N, Miyakawa Y, Yagi Y, Kreiter VP, Pressman D (1973) Radioiodinated soluble HLA antigens: HLA alloantigenic characterization and use in radioimmunoassay. J Immunol Methods 3: 109–126PubMedCrossRefGoogle Scholar
  2. 2.
    Tanigaki N, Tosi R (1982) Assessment of the specificity of human la alloantisera and alloantigens by the use of radioiodinated human la antigens. Tissue Antigens 20: 1–21PubMedCrossRefGoogle Scholar
  3. 3.
    Tosi R, Tanigaki N, Centis D, Rossi PL, Alfano G, Ferrara GB, Pressman D (1980) HLA-DR typing by radio-immunoassay. Transplantation 29: 302–305PubMedCrossRefGoogle Scholar
  4. 4.
    Tanigaki N, Tosi R (1982) The genetic control of human la alloantigens: A three-loci model derived from the immunochemical analysis of ‘supertypic’ specificities. Immunol Rev 66: 5–37PubMedCrossRefGoogle Scholar
  5. 5.
    Tosi R, Tanigaki N, Centis D (1980) Analysis at molecular level of the specificity of 8th workshop antisera. In: Terasaki PI (ed) Histocomatibility testing 1980. UCLA Tissue Typing Laboratory, Los Angeles, pp 903–906Google Scholar
  6. 6.
    Tanigaki N, Tosi R, Sagawa K, Minowada J, Ferrara GB (1983) The distribution of DR5, MT2, and MB3 specificities on human la subsets. Immunogenetics 17: 371–386PubMedCrossRefGoogle Scholar
  7. 7.
    Tosi R, Tanigaki N, Centis D, Ferrara GB, Pressman D (1978) Immunological dissection of human la molecules. J Exp Med 148: 1592–1611PubMedCrossRefGoogle Scholar
  8. 8.
    Tanigaki N, Tosi R, Katagiri M, Ferrara GB (to be published) A DC specificity associated with DR4 in single linkage disequilibrium. Immunogenetics (submitted)Google Scholar
  9. 9.
    Tanigaki N, Tosi R, Duquesnoy RJ, Ferrara GB (1983) Three la species with different structures and alloanti-genic determinants in an HLA-homozygous cell line. J Exp Med 157: 231–247PubMedCrossRefGoogle Scholar
  10. 10.
    Tanigaki N, Tosi R, Pressman D, Ferrara GB (1980) Molecular identification of human la antigens coded for by a gene locus closely linked to HLA-DR locus. Immunogenetics 10: 151–167PubMedCrossRefGoogle Scholar
  11. 11.
    Tosi R, Vismara D, Tanigaki N, Ferrara GB, Cicimarra F, Buffalano W, Folio D, Auricchi S (1983) Evidence that celiac disease is primarily associated with a DC locus allelic specificity. Clin Immunol Immunopathol 28: 395–403PubMedCrossRefGoogle Scholar
  12. 12.
    Tanigaki N, Tosi R, Centis D, Ferrara GB (to be published) DR5-associated DC molecules carry three different allospecificities. Hum Immunol (in press)Google Scholar
  13. 13.
    Tosi R, Tanigaki N, Sorrentino R, Centis D, Ferrara GB (1984) Serologically detectably polymorphism of the HLA-DC α-subunit. J Immunol 132: 277–282PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • N. Tanigaki
    • 1
  • P. Overturf
    • 1
  • L. Rendina
    • 1
  • J. Shaver
    • 1
  • R. Tosi
    • 2
  • G. B. Ferrara
    • 3
    • 4
  1. 1.Department of Molecular ImmunologyRoswell Park Memorial InstituteBuffaloUSA
  2. 2.Laboratorio di Biologia CellulareCNRRomaItaly
  3. 3.Istituto per Studio et la Cura dei TumoriGenovaItaly
  4. 4.AVISBergamoItaly

Personalised recommendations