Specificity of the Ninth Workshop Anti Ia Alloantisera as Assessed by the Direct Binding Assay
Antibody specificity can be easily defined when the binding of antibody to antigen is measured directly. A sensitive determination of such direct binding is possible by the use of radiolabeled antigen. This is also the case for alloantigens [1, 2]. We have been using 125I-labeled la antigens for specificity assay of the alloantisera and more recently of the monoconal antibodies. The assay method (we call it the direct binding assay) first allows reaction of a test alloantiserum or a monoclonal antibody with a radioiodinated la preparation, and then measures the extent of binding by the double-antibody technique . When a panel of 125I-labeled la preparations of different specificities is used, the major or putative antibody specificity can be deduced from the reaction pattern. This assay has been found particularly useful in the search for antibodies that can be used for la typing by the binding inhibition assay [2, 3] and also for immunospecific isolation of la molecules carrying different specificities .
KeywordsFiltration Prep Clarification Papain Cura
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