HLA-A, B, C Monoclonal Antibodies

  • R. Fauchet
  • J. G. Bodmer
  • L. J. Kennedy
  • M. C. Mazzilli
  • C. Müller
  • C. Raffoux
  • R. Richiardi

Abstract

Since Köhler and Milstein (1975) perfected the hybridroma technique, it has been possible to produce monoclonal antibodies (Mo Abs) which react against antigens of the human MHC. This technology allowed the production of some polymorphic reagents, of which 54 HLA-A and B Mo Abs were selected for the Ninth Workshop; 49 of these had clear HLA-A or B specificity assigned by the submitting laboratory. Two analyses were carried out. The first used 5000 unrelated individuals including 3000 Caucasoids, 1500 Japanese, and 500 Negroes. The second was performed on data from all British laboratories, selected European laboratories (ARN, BAR, FAU, FER, GAN, JEA, VIV, IT 1, CRB, CON, MTT, MEN, SAV), South African laboratories (DUT, HAM), and Japanese laboratories (TOK, KSH, OKO, SAS).

Keywords

Europe Shipping Stein Sorting Azide 

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References

  1. 1.
    Berger AE, Davis JE, Cresswell P (1982) Monoclonal anti-body to HLA-A3. Hybridoma 1: 87–90PubMedCrossRefGoogle Scholar
  2. 2.
    Bourel D, Fauchet R, Genetet B, Merdrignac G, Hurez D (1982) A monoclonal antibody to HLA-A3 allo-antigen. Immunol Letters 5: 233–237CrossRefGoogle Scholar
  3. 3.
    Trabace S, Mazzilli MC, Cascino I, Lulli SP, Costanzi S, Costanzi PS, Gandini E (1984) A mouse monoclonal anti-body detecting the allospecificity HLA-A3. Tissue Antigens 23: 12–16PubMedCrossRefGoogle Scholar
  4. 4.
    Belvedere M, Richiardi P, Luciani G, Curtoni ES (1980) Crossreactions among HLA-A antigens particularly focused on HLA-A28. In: Terasaki PI (ed) Histocompatibility testing 1980. UCLA, Los Angeles, p 751Google Scholar
  5. 5.
    Kennedy LJ, Bodmer JG (1983) A2 variants including A2/A28 (a new A-locus antigen) defined by monoclonal antibodies ( MCA ). Ninth Workshop Newsletter V: 1–3Google Scholar
  6. 6.
    Miiller C, Liangru S, Schneider M, Ziegler A, Wernet P (1983) A cytotoxic monoclonal IgM antibody (Tü1O1) directed against an antigenic determinant shared between the HLA-A allospecificities A2 and A28. Hum Immunol 6: 189–197CrossRefGoogle Scholar
  7. 7.
    Grumet FC, Fendly VM, Fish L, Foung S, Engleman EG (1982) A monoclonal antibody (B27M2) subdividing HLA-B27. Hum Immunol 5: 61–72PubMedCrossRefGoogle Scholar
  8. 8.
    Sato K, Kusama M, Yamashita Y, Nose Y, Sukegawa T, Tsuji K (1984) Possible supertypic HLA-A antigen detected by monoclonal antibodies in Japanese. Ninth Work-shop Newsletter 6: 26Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • R. Fauchet
    • 1
  • J. G. Bodmer
    • 2
  • L. J. Kennedy
    • 2
  • M. C. Mazzilli
    • 3
  • C. Müller
    • 4
  • C. Raffoux
    • 5
  • R. Richiardi
    • 6
  1. 1.Laboratoire d’HistocompatibilitéCentre Regional de Transfusion SanguineRennesFrance
  2. 2.Imperial Cancer Research FundLondonUK
  3. 3.Cathedra di Genetica MedicaUniversità la SpeziaRomeItaly
  4. 4.HLA-Serologie Abt. IIImmunologisches LaborTübingenFederal Republic of Germany
  5. 5.Hôpital St. LouisParisFrance
  6. 6.Istituto di Genetica MedicaUniversità di TorinoTorinoItaly

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