Skip to main content

Biochemistry of Class II Antigens: Workshop Report

  • Conference paper
Histocompatibility Testing 1984

Abstract

The structure of the HLA-D region has been enormously clarified by the results of recent gene cloning studies [1–4]. These results, when combined with those of cellular responses, serology and immunoprecipitation, lead to the suggested genetic complement and arrangement shown in Fig. 1.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Auffray C, Lillie JW, Arnot C, Grossberger D, Kappes D, Strominger JL (1983) Isotopic and allotypic variation of human class II histocompatibility antigen a chain genes. Nature 308: 327 - 333

    Article  Google Scholar 

  2. Larhammar D, Hyldig-Nielsen JJ, Servenius B, An- dersson G, Rask L, Peterson PA (1983) Exon-intron organization and complete nucleotide sequence of a human major histocompatibility antigen DCβ gene. Proc Natl Acad Sci USA 80: 7313–7317

    Article  PubMed  CAS  Google Scholar 

  3. Long EO, Wake CT, Gorski J, Mach B (1983) Complete sequence of an HLA-DRβ chain decuded from a cDNA clone and identification of multiple non-allelic DRβ chain genes. EMBO J 2: 389–394

    PubMed  CAS  Google Scholar 

  4. Spielman RS, Lee J, Bodmer WF, Bodmer JG, Trows- dale J (1984) Six HLA-Da chain genes on human chromosome 6: polymorphisms and associations of DCα-related sequences with DR types. Proc Natl Acad Sci USA 81: 3461–3465

    Article  PubMed  CAS  Google Scholar 

  5. Kaufman JF, Auffray C, Korman AJ, Shackelford DA, Strominger J (1984) The class II molecules of the human and murine major histocompatibility complex. Cell 36: 1–13

    Article  PubMed  CAS  Google Scholar 

  6. Hurley CK, Giles RC, Nunez G, DrMars R, Nadler L, Winchester R, Statsny P, Capra JD (1984) Molecular localization of human class II MT2 and MT3 determinants. J Exp Med 160: 472–493

    Article  PubMed  CAS  Google Scholar 

  7. Suzuki M, Maeda H, Mukai R, Yabe T, Hamaguchi U (1983) Identification of the MT3 molecule using two- dimensional gel electrophoresis and alloantisera. Immunogenetics 18: 575–583

    Article  PubMed  CAS  Google Scholar 

  8. Jones PP, Murphy DB, Hewgill D, McDevitt HO (1978) Detection of a common polypeptide chain in I-A and I-E sub-region immunoprecipitates. Mol Immunol 16: 51–60

    Article  Google Scholar 

  9. Charron DJ, McDevitt HO (1979) Analysis of HLA-D region associated molecules with monoclonal antibody. Proc Natl Acad Sci USA 79: 6567–6571

    Article  Google Scholar 

  10. Rudd CE, Bodmer JG, Bodmer WF, Crumpton MJ (1984) HLA-D region antigen associated invariant poly-peptides as revealed by 2-D gel analysis: glycosylation and structural interrelationships. J Biol Chem (in press)

    Google Scholar 

  11. Strubin M, Mach B, Long EO (1984) The complete sequence of the mRNA for the HLA-DR-associated invariant chain reveals a polypeptide with an unusual transmembrane polarity. EMBO J 3: 869–872

    PubMed  CAS  Google Scholar 

  12. Owen MJ, Kissonerghis AM, Lodish HF, Crumpton MJ (1981) Biosynthesis and maturation of HLA-DR antigens in vivo. J Biol Chem 256: 8987–8993

    PubMed  CAS  Google Scholar 

  13. Shackelford DA, Mann D, van Rood JJ, Ferrara GB, Strominger JL (1981) Human B-cell alloantigens DC1, MT1 and LB 12 are identical to each other but distinct from the HLA-DR antigen. Proc Natl Acad Sci USA 78: 4566–4590

    Article  PubMed  CAS  Google Scholar 

  14. de Kretser TA, Crumpton MJ, Bodmer JG, Bodmer WF (1982) Two-dimensional gel analysis of the polypeptides precipitated by a polymorphic HLA-DR 1,2,w6 mono-clonal antibody: evidence for a third locus. Eur J Immunol 12: 600–606

    Article  PubMed  Google Scholar 

  15. de Kretser TA, Crumpton MJ, Bodmer JG, Bodmer WF (1983) Biochemical analysis of the polymorphism of three HLA-D region antigens. Mol Biol Med 1: 59–76

    PubMed  Google Scholar 

  16. Adams TE, Bodmer JG, Bodmer WF (1983) Production and characterisation of monoclonal antibodies recognising the a-chain subunits of human la alloantigens. Immunology 50: 613–624

    PubMed  CAS  Google Scholar 

  17. Lampson LA, Levy R (1980) Two populations of la-like molecules on a human B cell line. J Immunol 125: 293–299

    PubMed  CAS  Google Scholar 

  18. Quaranta V, Indiveri F, Glassy MC, Ng A, Russo C, Molinaro GA, Pellegrino MA, Ferrone S (1980) Serological, functional, and immunochemical characterization of a monoclonal antibody (MoAb Q2/70) to human la-like antigens. Hum Immunol 3: 211–223

    Article  Google Scholar 

  19. van Heyningen V, Guy K, Newman R, Steel CM (1982) Human MHC class II molecules as differentiation markers. Immunogenetics 16: 459–469

    Article  PubMed  Google Scholar 

  20. Hurley CK, Shaw S, Nadler L, Schlossman S, Capra JD (1982) Alpha and beta chains of SB and DR antigens are structurally distinct. J Exp Med 156: 1557–1562

    Article  PubMed  CAS  Google Scholar 

  21. Ozato K, Mayer N, Sachs DH (1980) Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and la antigens. J Immunol 124: 533–540

    PubMed  CAS  Google Scholar 

  22. Wernet P, Ziegler A, Shaw S, Blaurock M, Pawelec G (1983) Monoclonal antibodies against la-like antigens inhibiting HLA-D and/or SB-directed secondary proliferative responses. Transplant Proc XV: 94–98

    Google Scholar 

  23. Bodmer J, Heyes J, Lindsay J (1984) Study of monoclonal antibodies to the HLA-D region products DQwl and DRw 52. This volume

    Google Scholar 

  24. Giles RC, Nunez G, Hurley CK, Nunez-Roldan A, Win-chester R, Stastny P, Capra JD (1983) Structural analysis of a human I-A homologue using a monoclonal antibody that recognises an MB3-like specificity. J Exp Med 157: 1461–1470

    Article  PubMed  CAS  Google Scholar 

  25. Goyert SM, Shively JE, Silver J (1982) Biochemical characterization of a second family of human la molecules, HLA-DS, equivalent to murine I-A subregion molecules. J Exp Med 156: 550–566

    Article  PubMed  CAS  Google Scholar 

  26. Makgoba MW, Hildreth JEK, McMichael AJ (1983) Identification of a human la antigen that is different from HLA-DR and DC antigens. Immunogenetics 17: 623–635

    Article  PubMed  CAS  Google Scholar 

  27. Watson AJ, DeMars R, Trowbridge IS, Bach FH (1983) Detection of a novel human class IIHLA antigen. Nature 304: 358–361

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1984 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Crumpton, M.J., Bodmer, J.G., Bodmer, W.F., Heyes, J.M., Lindsay, J., Rudd, C.E. (1984). Biochemistry of Class II Antigens: Workshop Report. In: Albert, E.D., et al. Histocompatibility Testing 1984. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69770-8_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-69770-8_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69772-2

  • Online ISBN: 978-3-642-69770-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics