Presence of Thyroid-Stimulating Immunoglobulins (TSI) in DR3 Positive Normal Individuals

  • J. Charreire
  • G. Karsenty
  • V. Lepage
  • L. Degos
Conference paper

Abstract

With the method of thyroid epithelial cell culture (TEC) that we developed in mice [1], monolayers of human TEC (HTEC) were used to detect thyroid- stimulating immunoglobulins (TSI) in a functional assay of cyclic AMP (cAMP) production. Under these experimental conditions, we previously demonstrated the presence of TSI in 92% of untreated Graves disease (GD) patients and their disappearance 6 months after neomercazole treatment in 55% of the GD patients [2]. In this study, TSI positivity was evaluated as the percentage of response when DEAE-trisacryl-purified IgGs from normal subjects were deposited onto HTEC for cAMP production. Among the 32 samples provided by the blood bank and used as controls, we found four TSI-positive subjects with euthyroid status.

Keywords

Thyroglobulin Neomercazole 

References

  1. 1.
    Salamero J, Michel-Béchet M, Charreire J (1983) Expression of H-2 antigens and thyroglobulin (Tg) on freshly dissociation and in vitro cultured monolayers of mouse thyroid epithelial cells ( TEC ). Tissue Antigens 22: 231–238PubMedCrossRefGoogle Scholar
  2. 2.
    Charreire J, Karsenty G, Bouchard P, Schaison B (1984) Effect of Neomercazole (NMZ) treatment in patients with Graves diseases ( GD ). Clin Exp Immunol 57: 633PubMedGoogle Scholar
  3. 3.
    Briones-Urbina R, Bear JC, Farid NR (1982) Association of hypergammaglobulinemia G with HLA-DR3 in Graves’ disease. Tissue Antigens 19: 20–24PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • J. Charreire
    • 1
  • G. Karsenty
    • 1
  • V. Lepage
    • 2
  • L. Degos
    • 2
  1. 1.INSERM U25Hôpital Necker Enfants MaladesParisFrance
  2. 2.INSERM U90Hôpital Saint-LouisParisFrance

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