HLA-DR and DQ, Studied with Genomic Blotting, in Diabetic Families and Transplant Donor-Recipient Pairs

  • B. Carlsson
  • J. Böhme
  • B. Kalm
  • G. Lundgren
  • E. Möller
  • B. Persson
  • P. A. Peterson
  • L. Rask
  • J. Wallin
Conference paper

Abstract

The DNA-DNA hybridization technique was employed to investigate the possible role of HLA class II genes other than DR in clinical kidney and bone marrow transplantation. Using cDNA probes corresponding to the polymorphic DRβ, DQβ, and DQα chains (Wallin et al., this volume), we examined the restriction fragment length polymorphism (RFLP) in PvuII- or BamHI-digested genomic DNA in bone marrow and kidney transplant donor- recipient pairs, and in 14 diabetic families. The RFLP detected is adequate to type for DR and DQ specificities [1; Wallin et al., this issue]. The bone marrow transplant donor-recipient pairs examined are all serologically HLA-A, B, C, DR identical sibs. Among those patients with good clinical outcome it was not possible to detect any difference in RFLP between the donor and the recipient with either DRβ or DQβ probes. However, in one donor-recipient pair a 1.6-kb Pvu II fragment visualized with the DQβ probe was present in the recipient and absent in the donor.

References

  1. 1.
    Andersson M, Böhme J, Andersson G, Möller E, Thorsby E, Rask L, Peterson PA (1984) Genomic hybridization with class II transplantation antigen cDNA probes as a complementary technique in tissue typing. Hum Immunol 11,2: 57–68Google Scholar
  2. 2.
    Owerbach D, Lernmark Å, Platz P, Ryder LP, Rask L, Peterson PA, Ludvigsson S (1983) HLA-D region chain DNA endonuclease fragments differ between HLA-DR identical healthy and insulin-dependent diabetic individuals. Nature 303: 815–817PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • B. Carlsson
    • 1
  • J. Böhme
    • 2
  • B. Kalm
    • 2
  • G. Lundgren
    • 3
  • E. Möller
    • 1
  • B. Persson
    • 4
  • P. A. Peterson
    • 2
  • L. Rask
    • 5
  • J. Wallin
    • 1
  1. 1.Department of Clinical ImmunologyHuddinge University HospitalHuddingeSweden
  2. 2.Department of Cell Research, Wallenberg LaboratoryUppsala UniversityUppsalaSweden
  3. 3.Department of Transplantation, SurgeryHuddinge University HospitalHuddingeSweden
  4. 4.Department of PediatricsSt. Göran University HospitalStockholmSweden
  5. 5.Swedish University for Agricultural SciencesUppsalaSweden

Personalised recommendations