Advertisement

DNA Polymorphism of HLA-DRβ and HLA-DPβ Genes

  • A. K. L. So
  • J. Trowsdale
  • J. G. Bodmer
  • W. F. Bodmer

Abstract

The existence of at least three sets of cell surface products encoded by the HLA-D region — DR, DQ and DP — has been established by serological, cellular biochemical and molecular studies [for reviews see 1, 2]. The initial definition of DR was based on alloantisera reactive against B cell determinants, which showed extensive polymorphism. Serological and biochemical studies of the cross-reactive determinants detected within the DR series indicated the existence of a second allelic series of cell surface products, DQ (DC or MB), which are in strong linkage disequilibrium with DR alleles. Subsequently, a third series, DP (SB), was defined by primed lymphocyte typing [3], and available biochemical studies show that it is distinct from DR and DQ [4]. A fourth set of products, closely related to DR, has been described [5]. They are probably the antigens recognised by the MT or BR group of apparently cross-reactive sera [6], and are also coded for in the DR subregion.

Keywords

Serological Type Serological Specificity Imperial Cancer Research Fund Murine Major Histocompatibility Complex Homozygous Typing Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bodmer WF, Bodmer JG, Lee J, So A, Spielman R, Travers P, Trowsdale J (1984) Molecular genetics of the HLA-D region. Gorer Symposium. J Immunogenet (in press)Google Scholar
  2. 2.
    Kaufman JF, Auffray C, Kouman AJ, Shackelford DA, Strominger JL (1984) The class II molecules of the human and murine major histocompatibility complex. Cell 36:1–13PubMedCrossRefGoogle Scholar
  3. 3.
    Shaw J, Kavathas P, Pollack MA, Charmot D, Mawas C (1981) Family studies define a new histocompatibility locus SB, between HLA-DR and GLO. Nature 293:745–747PubMedCrossRefGoogle Scholar
  4. 4.
    Shackelford DA, Kaufman JF, Kouman AJ, Strominger JL (1982) HLA-DR antigens: structure, separation of subpopulations. Gene cloning and function. Immunol Rev 66:133–187PubMedCrossRefGoogle Scholar
  5. 5.
    Tanigaki N, Tosi R, Duquesnoy RJ, Ferrara GB (1983) Three la species with different structures and alloantigenic determinants in an HLA-homozygous cell line. J Exp Med 157:231–247PubMedCrossRefGoogle Scholar
  6. 6.
    Shackelford DA, Strominger JL (1984) Identification of three class II antigens, DR7, MB2, and MT3 from a homozygous human cell line. Hum Immunol 9:159–174PubMedCrossRefGoogle Scholar
  7. 7.
    Wake CT, Long EO, Mach B (1982) Allelic polymorphism and complexity of the genes for HLA-DRß chains. Nature 300:372–374PubMedCrossRefGoogle Scholar
  8. 8.
    Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517PubMedCrossRefGoogle Scholar
  9. 9.
    Spielman R, Lee J, Bodmer WF, Bodmer J, Trowsdale J (1984) Six HLA-D region α chain genes on human chromosome 6. Proc Natl Acad Sci USA 81:3461–3465PubMedCrossRefGoogle Scholar
  10. 10.
    Trowsdale J, Lee J, Carey J, Grosveld F, Bodmer J, Bodmer WF (1983) Sequences related to HLA-DRα chains on human chromosome 6. Proc Natl Acad Sci USA 80:1972–1976PubMedCrossRefGoogle Scholar
  11. 11.
    de Kretser TA, Crumpton MJ, Bodmer JG, Bodmer WF (1982) Two-dimensional gel analysis of the polypeptides precipitated by a polymorphic HLA-DR 1, 2 and w6 monoclonal antibody. Eur J Immunol 12:600–606PubMedCrossRefGoogle Scholar
  12. 12.
    Long EO, Wake CT, Strubin M, Gross N, Aciolla R, Carrel S, Mach B (1982) Isolation of distinct cDNA clones encoding HLA-DRß chains by use of an expression assay. Proc Natl Acad Sci USA 79:7465–7469PubMedCrossRefGoogle Scholar
  13. 13.
    Trowsdale J, Kelly A, Lee J, Carson S, Austin P, Travers P (1984) A linkage map of two SBα and ß related genes. Cell 38:241–249PubMedCrossRefGoogle Scholar
  14. 14.
    Owerbach D, Lernmark A, Rask L, Peterson PA, Platz P, Svejgaard A (1983) Detection of HLA-D/DR-related DNA polymorphism in HLA-D homozygous typing cells. Proc Natl Acad Sci USA 80:3758–3761PubMedCrossRefGoogle Scholar
  15. 15.
    Bodmer WF, Bodmer J, Lee J, Spielman R, Travers P, Trowsdale J (1984) Structure, evolution and polymorphism of the HLA-D region. Immunogenetics - its application to clinical medicine. Academic, London (in press)Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • A. K. L. So
    • 1
  • J. Trowsdale
    • 1
  • J. G. Bodmer
    • 1
  • W. F. Bodmer
    • 1
  1. 1.Imperial Cancer Research Fund LaboratoriesLincoln’s Inn FieldsLondonUK

Personalised recommendations