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Molecular Dissection of DRw6

  • A. Haziot
  • V. Lepage
  • L. Degos
  • D. J. Charron

Abstract

The complex HLA-DRw6 specificity was first described at the Seventh Histocompatibility Workshop according to the reactivity of a set of polyspecific sera (DR2 + w6; DR3 + w6). While the DR specificities 1 to 5 and 7 were clearly defined during the following workshop, no monospecific anti-DRw6 sera were found. HLA-DRw6 was therefore only defined by a serie of supertypic sera. These include anti-DQw1, which react with DR1, DR2, and DRw6; anti-DRw52 which react with DR3, DR5, DRw6, and DRw8; and sera with shorter reactivity: anti-DR2+w6, anti-DR3+w6, anti-DR5+w6, anti-DR3+5+w6. DRw6 specificity was thus assigned to cells reacting with the above supertypic sera but negative with monospecific sera anti-DR1, 2, 3, 5, and w8. Subdivisions of DRw6 were proposed following combinations of reactivities with clusters of supertypic sera as reviewed in a recent report [7]. Because of the absence of monospecific DRw6 sera the existence of the DRw6 molecule itself has been a matter of controversy. We investigated the molecular nature of the DRw6 antigens in 15 individuals and four families using a monomorphic monoclonal anti-HLA-DR antibody and two-dimensional gel electrophoresis (2D-PAGE).

Keywords

Chain Pattern Serum 9W781 Monospecific Seron DRw6 Antigen DRw6 Haplotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • A. Haziot
    • 1
    • 2
  • V. Lepage
    • 2
  • L. Degos
    • 2
  • D. J. Charron
    • 1
  1. 1.Laboratoire d’ImmunogénétiqueDépartement d’Hématologie CHU Pitié SalpétrièreParisFrance
  2. 2.INSERM U.93, Hôpital Saint-LouisParis Cedex 10France

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