Construction and Expansion of CTLs for HLA Typing: Definition of B44 Subtypes by Cellular Typing

  • P. W. Hansen
  • S. E. Christiansen
  • F. Kissmeyer-Nielsen
  • T. Kristensen


The polymorphic gene products of the human HLA region can be identified both by serological and cellular typing techniques. Although the definition of the system, its loci, and their antigenic determinants is in essence based on serological studies and analyses, it is now evident that a determinant primarily described by one technique, e.g., serology, may be detected similarly by the other, viz cellular typing (MLC, PLT, or CML). Thus, serological and cellular typing studies are equally precise with regard to specificity and must supplement each other.


Cellular Typing Lysed Target Cell Negative Target Percentage Cytotoxicity Reciprocal Combination 
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  1. 1.
    Mawas C, Christen Y, Legrand L, Sasportes M, Dausset J (1974) Cellular and humoral response against determinants other than the classical HL-A specificities. Transplantation 18: 256–266PubMedCrossRefGoogle Scholar
  2. 2.
    Kristensen T, Grunnet N, Kissmeyer-Nielsen F (1974) Occurrence of unexpected HL-A ( LA and FOUR) irrelevant lympholysis. Tissue Antigens 4: 378–382PubMedCrossRefGoogle Scholar
  3. 3.
    Schendel DJ, Wank R, Hansen JA, Dupont B (1977) Cell-mediated lympholysis in man: identification of a common target determinant associated with HLA-B12 and Bw35. Transplant Proc 9: 1777–1780PubMedGoogle Scholar
  4. 4.
    Goulmy E, Termijtelen A, Bradley BA, van Rood J (1976) HLA restriction of non-HLA-A, -B, -C and -D cell mediated lympholysis. Tissue Antigens 8: 317–326PubMedCrossRefGoogle Scholar
  5. 5.
    Biddison WE, Krangel MS, Strominger JL, Ward FE, Shearer GM, Shaw S (1980) Virus-immune cytotoxic T cells recognize structural differences between serologically indistinguishable HLA-A2 molecules. Hum Immunol 1: 225–232PubMedCrossRefGoogle Scholar
  6. 6.
    Kato S, Ivanyi P, Lacko E, Breur B, du Bois R, Eijsvoogel VP (1982) HLA-B locus (B12) antigen variants defined by CTL generated between B locus-identical (B12) responder-stimulator pairs. J Immunol 128: 949–955PubMedGoogle Scholar
  7. 7.
    Breuning MH, Lucas CJ, Breur BS, Engelsma MY, de Lange GG, Dekker AJ, Biddison WE, Iványi P (1982) Subtypes of HLA-B27 detected by cytotoxic T lymphocytes and their role in self-recognition. Hum Immunol 5: 259–268PubMedCrossRefGoogle Scholar
  8. 8.
    van der Poel JJ, Mölders H, Thompson A, Ploegh HL (1983) Definition of four HLA-A2 subtypes by CML typing and biochemical analysis. Immunogenetics 17: 609–621PubMedCrossRefGoogle Scholar
  9. 9.
    Goulmy E, van Leeuwen A, Blokland E, van Rood JJ, Biddison WE (1982) Major histocompatibility complex - restricted H-Y-specific antibodies and cytotoxic T lymphocytes may recognize different self determinants. J exp Med 155: 1567–1579PubMedCrossRefGoogle Scholar
  10. 10.
    Tekolf AW, Biddison WE, Aster RD, Shaw S (1982) Two subgroups of HLA-Bw44 defined by cell-mediated lympholysis that differ in Bw44 expression on platelets and in patterns of genetic linkage disequilibrium. J Immunol 129: 1474–1478PubMedGoogle Scholar
  11. 11.
    Hahn AWA, Schendel DJ, Hansen PW, Ploegh HL (1984) Biochemical and cellular identification of HLA- Bw44 subtypes. Hum Immunol 11: 69–76PubMedCrossRefGoogle Scholar
  12. 12.
    Gillis S, Baker PE, Ruscetti FW, Smith KA (1978) Long-term culture of human antigen specific cytotoxic T cell lines. J Exp Med 148: 1093–1101PubMedCrossRefGoogle Scholar
  13. 13.
    Kristensen T, Malissen B, Johnsen HE (1982) Histocompatibility typing by polyclonal and cloned human cytotoxic T lymphocytes. In: Ferrone S, Solheim BG (eds) HLA typing: methodology and clinical aspects, vol 2. CRC Press, Florida, pp 123–150Google Scholar
  14. 14.
    European CML Study Group (1980) Human histocompatibility testing by T cell-mediated lympholysis: a European standard CML technique. Tissue Antigens 16: 335–367Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • P. W. Hansen
    • 1
  • S. E. Christiansen
    • 1
  • F. Kissmeyer-Nielsen
    • 1
  • T. Kristensen
    • 1
  1. 1.The Tissue Typing LaboratoryUniversity Hospital of AarhusAarhusDenmark

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