Human T Cell Clone Inhibition by Distinct Monoclonal Anti-HLA-DR Antibodies
Inhibition studies of the specific proliferative response of T cell clones with different anti-HLA-DR monoclonal antibodies (moAb) should help to relate the structure and the function of multiple DR epitopes. We now describe the inhibition of diphtheria toxoid- and Varidase-specific T cell clone proliferative responses in a single DRw6/7 donor by eight distinct monoclonal anti-HLA-DR moAbs. These moAbs were selected because they have already been biochemically characterized. MoAbs 141, D1.12, VI-15 C, and 157, which detected very similar epitopic specificities, immunoprecipitated only DR molecules on DR 1 - w8. MoAbs 206 and 135, which detected different epitopes, recognized DR (I-E-like) molecules in most of the alleles and also DQ (I-A-like) molecules in DR7 individuals. BM 50 recognized molecules of all alleles except DR7 and the corresponding subgroup of DRw6. MoAbs 141 and 135, 157, and BM 50 shared idiotypic specificities.