Class I MHC-Linked Determinants Expressed on Activated T Cells
Sensitized T-cells in mixed leukocyte culture possess HLA-D like antigens absent from nonactivated resting T cells. These antigens are synthesized by allostimulated T cells . T cell expression of HLA- DR antigens has been demonstrated by several investigators using a variety of methods [2–6]. On activated T cells, specific anti-HLA-DR antisera showed unexplained reactivity, distinguishable from HLA-DR reactivity . This antiblast activity had also been found in sera from multiparous women in which no anti-HLA-A, B, or DR antibodies were detectable . Gazit and co-workers postulated the existence of a genetic system specific to activated T cells, in close linkage disequilibrium with the HLA system. They named this the HT system and suggested that it might be a human correlate of the mouse TLa region. Two possible alleles of the postulated HT locus (HT1 or K, HT3 or SF48) were identified [9, 10]. The genetic polymorphism of the HT locus has recently been confirmed . The present study was undertaken to determine if sera from multiparous women clearly detected new determinants on blast cells different from the classical HLA-A, B, C antigens and similar to HTs. We also carried out this study to investigate the specificity of such determinants and their correlation with the HLA system.
KeywordsAmide Electrophoresis Polypeptide Turkey Polyacrylamide
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