CML: A Method for Cellular Typing
Cell-mediated lympholysis (CML) can be used as a cellular typing method to identify surface molecules that are recognized by cytotoxic T lymphocytes (CTLs). The target molecules recognized by CTLs in many cases correlated with HLA specificities (either class I or class II) that can be defined with serological reagents. On the other hand, several studies have clearly demonstrated that CTLs can detect HLA determinants that do not correspond to known serological specificities [1–9]. In several cases the CTL-defined specificities have been shown biochemically to represent distinct class I molecular variants, for example for HLA- A2, B27, and B44 [10–13]. Other CTLs have been described which show lysis of allogeneic target cells that share no serologically detectable HLA-A, B, C, or DR specificities with the sensitizing cells, and further investigation will be required to define the genetic and molecular basis of these anomalous CML reactions [14–16]. It is certainly clear that CTLs are unique reagents for identifying minor histocompatibility antigens in man and for defining the molecular epitopes important in HLA restriction [17–20].
KeywordsSpontaneous Release Tissue Antigen Local Assignment Minor Histocompatibility Antigen International Histocompatibility Workshop
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- 1.Kristensen T, Grunnet N, Kissmeyer-Nielsen F (1974) Cell mediated lympholysis in man. Occurrence of unexpected, HLA (“LA” and “Four”) irrelevant lympholysis. Tissue Antigens 4: 378–382Google Scholar
- 4.Kristensen T, Grunnet N (1975) Cell mediated lympholysis in man. Evidence of a separate CML locus within the major histocompatibility complex and an approach to CML typing. In: Kissmeyer-Nielsen F (ed). Histocompatibility testing 1975. Munksgaard, Copenhagen, p 835Google Scholar
- 5.Goulmy E, Termijtelen A, Bradley B, van Rood JJ (1976) HLA restriction of non-HLA-A, -B, -C, and -D cell me-diated lympholysis ( CML ). Tissue Antigens 8: 317–326Google Scholar
- 6.Long MA, Handwerger BS, Amos DB, Yunis EJ (1976) The genetics of cell mediated lympholysis. J Immunol 177: 2092–2099Google Scholar
- 11.van der Poel JJ, Molders HH, Thompson A, Ploegh H. (1983) Definition of four HLA-A2 subtypes by CML typing and biochemical analysis. Immunogenetics 17: 603–608Google Scholar
- 13.Hahn AW, Schendel DJ, Wendelboe Hansen P, Ploegh H. Biochemical and cellular identification of HLA- Bw44 subtypes. Hum Immunol (in press)Google Scholar
- 14.Ward MJ, Stiller CR, McGirr DA, Fung M, Cousins HK, Paty DW, Hawson WT, Sinclair NRSTC (1977) CML problems and possibilities defining specificities. Trans-plant Proc 9: 1781–1784Google Scholar
- 22.European CML Study Group (1980) Human his-tocompatibility testing by T cell mediated lympholysis: a European standard CML technique. Tissue Antigens 16: 335–367Google Scholar
- 23.Breuning MH, Tekolf WA, Kato S, Breur BS, Engelsma MY, Spits H, Ivanyi P (1983) High resolution HLA typing by cytotoxic T lymphocytes. Transplant Proc 15: 118–122Google Scholar