31P-NMR Studies in Scenedesmus C-2A’ in Darkness and Blue Light
Blue light stimulates carbohydrate degradation and respiration of green algae (review: [8, 10, 12]); the underlying mechanism, however, is not well understood. Since all reactions are enzyme-catalyzed, enzymes are certainly candidates for regulation. Indeed several enzymes have been reported to be blue light-stimulated (see Ruyters this Vol.). The finding of a blue light-mediated enhancement of PEP carboxylase and pyruvate kinase activity [6, 9], together with the reported blue light-enhanced carbohydrate breakdown of a chlorophyll-free Chlorella mutant under anaerobic conditions  strongly indicates an important role of the glycolytic pathway in these blue light phenomena. Since the demonstrated changes in maximal catalytic activities of pyruvate kinase and PEPCase occur too slowly to account for the quick stimulation of respiration and carbohydrate breakdown , these processes might be regulated by changes in the concentration of effectors, cofactors and substrate of regulatory enzymes. However, except for adenosine phosphates , no data on the level of these compounds and their possible changes in blue light are available.
KeywordsStarch Chlorophyll Respiration Pyruvate Photosynthesis
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