Liver Glutaminase

  • J. D. Mcgivan
  • N. M. Bradford
  • A. J. Verhoeven
  • A. J. Meijer


The original observation that extracts of liver contain considerable gluta- mine-hydrolysing activity was reported by Krebs in 1935 [1]. Errera [2] demonstrated that the major part of the glutaminase activity was dependent on added phosphate and was located in the particulate fraction of the liver. Definitive evidence that glutaminase is a mitochondrial enzyme was first obtained by Guha [3]. Liver extracts also contain a phosphate-independent glutamine-hydrolysing activity located in the supernatant fraction [2, 4–6]. This activity is stimulated by maleate and represents a partial reaction of γ -glutamyltransferase (EC [7]. In a careful reinvestigation of the characteristics of glutamine hydrolysis in rat liver extracts, Horowitz and Knox [8] showed that 90% of the glutamine hydrolysing activity was phosphate-de- pendent and located in the mitochondria and was thus catalysed by the enzyme now classified as glutaminase (EC


Liver Mitochondrion Glutamine Metabolism Urea Synthesis Carbamoylphosphate Synthetase Glutaminase Activity 
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Copyright information

© Springer- Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • J. D. Mcgivan
  • N. M. Bradford
    • 1
  • A. J. Verhoeven
    • 2
  • A. J. Meijer
    • 2
  1. 1.Department of BiochemistryUniversity of Bristol Medical SchoolBristolEngland
  2. 2.Laboratory of Biochemistry, B.C.P. Jansen InstituteUniversity of AmsterdamAmsterdamThe Netherlands

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