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Liver Glutaminase

  • J. D. Mcgivan
  • N. M. Bradford
  • A. J. Verhoeven
  • A. J. Meijer

Abstract

The original observation that extracts of liver contain considerable gluta- mine-hydrolysing activity was reported by Krebs in 1935 [1]. Errera [2] demonstrated that the major part of the glutaminase activity was dependent on added phosphate and was located in the particulate fraction of the liver. Definitive evidence that glutaminase is a mitochondrial enzyme was first obtained by Guha [3]. Liver extracts also contain a phosphate-independent glutamine-hydrolysing activity located in the supernatant fraction [2, 4–6]. This activity is stimulated by maleate and represents a partial reaction of γ -glutamyltransferase (EC 2.3.2.2) [7]. In a careful reinvestigation of the characteristics of glutamine hydrolysis in rat liver extracts, Horowitz and Knox [8] showed that 90% of the glutamine hydrolysing activity was phosphate-de- pendent and located in the mitochondria and was thus catalysed by the enzyme now classified as glutaminase (EC 3.5.1.2)

Keywords

Liver Mitochondrion Glutamine Metabolism Urea Synthesis Carbamoylphosphate Synthetase Glutaminase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer- Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • J. D. Mcgivan
  • N. M. Bradford
    • 1
  • A. J. Verhoeven
    • 2
  • A. J. Meijer
    • 2
  1. 1.Department of BiochemistryUniversity of Bristol Medical SchoolBristolEngland
  2. 2.Laboratory of Biochemistry, B.C.P. Jansen InstituteUniversity of AmsterdamAmsterdamThe Netherlands

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