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Compartmentation of Ca2+ and its Possible Role in Volume Regulation of Poterioochromonas

  • H. Kauss
  • U. Rausch
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

There is growing evidence from animal systems that the concentration of free Ca2+ in the extra-organelle cytoplasm of resting cells is in the range of 10-7 M. External stimuli (e.g., antibody binding, light, hormones, nerve pulses) can induce an increase in the [Ca2+]cyt which in turn regulates many metabolic and developmental steps, rendering Ca2+ an important “second messenger”. Indirect evidence has often led to the suggestion, that Ca2+ may be equally important for plant cells, although in most cases measurements of [Ca2+]cyt and its changes have not been performed (Williamson 1981). There appears to be only one plant example where direct measurements of [Ca2+]cyt are available, namely internodal cells of Chara (Williamson and Ashley 1982). Using injection of the photoprotein aequorin the [Ca2+]cyt was found to be about 0.2 μM and was raised by electrical stimulation to 7 μM with a concommitant cessation of cytoplasmic streaming. Total Ca2+ in the cytoplasm of Chara is 2-8 mM, indicating that great amounts must be sequestered in organelles (as far as known mainly in mitochondria and endoplasmatic reticulumn, and vesicles derived from it). According to the above reference, short-term regulation of [Ca2+]cyt appears to occur in Chara at transport processes into and out of these organelles. Long-term regulation additionally involves transport over the tonoplast and plasma membrane, as the vacuolar and external concentrations of Ca2+ are in the mM range.

Keywords

Cell Shrinkage Cytoplasmic Streaming Storage Vacuole Internodal Cell Algal Pellet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

CTC

chorotetracycline

DCF

dichlofluanide

DMSO

dimethylsulfoxide

EGTA

ethylene glycol-bis-(2-aminoethyl ether)-N,N,N’,N’,-tetraacetic acid

IF

isofloridoside = α-D-galactosyl-1 → 1-glycerol

IFP

α-D-galactosyl-1 → 1-glycerol-3-phosphoric acid

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Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • H. Kauss
    • 1
  • U. Rausch
    • 1
  1. 1.FB BiologieUniversität KaiserslauternKaiserslauternGermany

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