The Transport Carrier Principle

  • W. D. Stein
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 70 / 1)

Abstract

The transport of metabolites across the intestinal epithelium has been a subject of absorbing interest to physiologists and pharmacologists for over a century. This present chapter deals with the carrier concept as applied to intestinal absorption, especially of sugars and amino acids. In this first section we shall consider the historical aspects of the development of the carrier concept and its elaboration for the understanding of intestinal absorption processes; Sect. B deals with the kinetics of carrier transport, while Sect. C is concerned with the efficiency and, hence, the energetics of carrier transport in absorbing systems.

Keywords

Permeability Hydrolysis Adenosine Choline Mannitol 

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References

  1. Alvarado F (1965) The relationship between Na+ and the active transport of arbutin in the small intestine. Biochim Biophys Acta 109:478–494PubMedCrossRefGoogle Scholar
  2. Aronson PS (1978) Energy dependence of phloridzin binding to isolated renal microvillus membranes. J Membr Biol 142:81–98Google Scholar
  3. Baker GF, Widdas WF (1973) The asymmetry of the facilitated transfer system for hexoses in human red cells and the simple kinetics of a two component model. J Physiol (Lond) 231:143–165Google Scholar
  4. Beauge LA, Glynn IM (1979) Occlusion of K ions in the unphosphorylated sodium pump. Nature 280:510–512PubMedCrossRefGoogle Scholar
  5. Bihler I, Crane RK (1962) Studies on the mechanism of intestinal absorption of sugars V. The influence of several cations and anions on the active transport of sugars in vitro, by various preparations of hamster small intestine. Biochim Biophys Acta 58:78–93CrossRefGoogle Scholar
  6. Britten HG (1977) Calculation of steady-state rate equations and the fluxes between substrates and products in enzyme reactions. Biochem J 161:517–526Google Scholar
  7. Cabantchik ZI, Ginsburg H (1977) Transport of uridine in human red cells. Demonstration of a simple carrier-mediated process. J Gen Physiol 69:75–96PubMedCrossRefGoogle Scholar
  8. Cass CE, Gaudette LA, Paterson ARP (1974) Mediated transport of nucleosides in human erythrocytes. Specific binding of the inhibitor nitrobenzethioinosine to nucleoside transport sites in the erythrocyte membrane. Biochim Biophys Acta 345:1–10PubMedCrossRefGoogle Scholar
  9. Christensen HN, Riggs TE, Ray NE (1952) Concentrative uptake of amino acids by erythrocytes in vitro. J Biol Chem 194:41–51PubMedGoogle Scholar
  10. Cleland WW (1963) The kinetics of enzyme-catalysed reactions with two or more substrates or products. Biochim Biophys Acta 67:104–187PubMedCrossRefGoogle Scholar
  11. Crane RK (1960) Intestinal absorption of sugars. Physiol Rev 40:789–825PubMedGoogle Scholar
  12. Crane RK (1962) Hypothesis of mechanism of intestinal active transport of sugars. Fed Proc 21:891–895PubMedGoogle Scholar
  13. Crane RK, Forstner G, Eichholz A (1965) Studies on the mechanism of the intestinal transport of sugars. X. An effect of Na+ concentration on the apparent Michaelis constants for intestinal sugar transport in vitro. Biochim Biophys Acta 109:467–477PubMedCrossRefGoogle Scholar
  14. Csáky TZ, Thale M (1960) Effect of ionic environment on intestinal sugar transport. J Physiol (Lond) 151:59–65Google Scholar
  15. Csáky TZ, Zollicoffer L (1960) Ionic effect on intestinal transport of glucose in the rat. Am J Physiol 198:1056–1058PubMedGoogle Scholar
  16. Csáky TZ, Hartzog HG, Fernald GW (1961) Effect of digitalis on active intestinal sugar transport. Am J Physiol 200:459–460PubMedGoogle Scholar
  17. Danielli JF (1954) The present position in the field of facilitated diffusion and selective active transport. Proc Symp Colston Res Soc 7: 1–4Google Scholar
  18. Davson H, Danielli JF (1943) The permeability of natural membranes. Cambridge University Press, CambridgeGoogle Scholar
  19. Deves R, Krupka RM (1978) A new approach in the kinetics of biological transport. The potential of reversible inhibition studies. Biochim Biophys Acta 510:186–200PubMedCrossRefGoogle Scholar
  20. Deves R, Krupka RM (1979) A simple experimental approach to the determination of carrier transport parameters for unlabeled substrate analogs. Biochim Biophys Acta 556:524–532PubMedCrossRefGoogle Scholar
  21. Edwards PAW (1972) Evidence for the carrier model in red cell glucose and choline transport. J Physiol (Lond) 225:36–37pGoogle Scholar
  22. Edwards PAW (1973a) The inactivation by fluorodinitrobenzene of glucose transport across the human erythrocyte membrane. The effect of glucose inside or outside the cell. Biochim Biophys Acta 307:415–418PubMedCrossRefGoogle Scholar
  23. Edwards PAW (1973b) Evidence for the carrier model of transport from the inhibition by N-ethylmaleimide of choline transport across the human red cell membrane. Biochim Biophys Acta 311:123–140PubMedCrossRefGoogle Scholar
  24. Edwards PAW (1974) A test for non-specific diffusion steps in transport across cell membranes and its application to red cell glucose transport. Biochim Biophys Acta 345:373–386CrossRefGoogle Scholar
  25. Glynn IM, Karlish SJD (1975) The sodium pump. Ann Rev Physiol 37:13–55CrossRefGoogle Scholar
  26. Goldner AM, Schultz SG, Curran PF (1969) Sodium and sugar fluxes across the mucosal border of rabbit ileum. J Gen Physiol 53:362–383PubMedCrossRefGoogle Scholar
  27. Heinz E, Geck P, Wilbrandt W (1972) Coupling in active transport. Activation of transport by co-transport and/or counter-transport with the fluxes of other solutes. Biochim Biophys Acta 225:442–461Google Scholar
  28. Höber R (1945) Physical chemistry of cells and tissues. Churchill, LondonGoogle Scholar
  29. Höber R, Höber J (1937) Experiments on the absorption of organic solutes in the small intestine of rats. J Cell Comp Physiol 10:401–422CrossRefGoogle Scholar
  30. Honig B, Stein WD (1978) Design principles for active transport systems. J Theor Biol 75:299–305PubMedCrossRefGoogle Scholar
  31. Hopfer U, Groseclose R (1980) The mechanism of Na+-dependent D-glucose transport. J Biol Chem 255:4453–4462PubMedGoogle Scholar
  32. Jencks WP (1980) The utilisation of binding energy in coupled vectorial processes. Adv Enzymol 51:75–106PubMedGoogle Scholar
  33. Jung CY (1974) Inactivation of glucose carriers in human erythrocyte membranes by 1-fluoro-2,4-dinitrobenzene. J Biol Chem 249:3568–3573PubMedGoogle Scholar
  34. Kessler M, Tannenbaum V, Tannenbaum C (1978) A simple apparatus for performing short-time (1–2 seconds) uptake measurements in small volumes. Its applications to D-glucose transport studies in brush border vesicles from rabbit jejunum and ileum. Biochim Biophys Acta 509:348–359PubMedCrossRefGoogle Scholar
  35. Koefoed-Johnsen V, Ussing HH (1958) Nature of the frog skin potential. Acta Physiol Scand 42:298–308PubMedCrossRefGoogle Scholar
  36. Krupka RM (1971) Evidence for a carrier conformational change associated with sugar transport in erythrocytes. Biochemistry 10:1143–1148PubMedCrossRefGoogle Scholar
  37. LeFevre PG (1948) Evidence of active transfer of certain non-electrolytes across the human red cell membrane. J Gen Physiol 31:505–527PubMedCrossRefGoogle Scholar
  38. Lieb WR (1982) A kinetic approach to transport studies. In: Ellory JC, Young JD (eds) Red cell membranes — a methodological approach. Academic, London, pp 135–164Google Scholar
  39. Lieb WR, Stein WD (1974a) Testing and characterising the simple pore. Biochim Biophys Acta 373:165–177PubMedCrossRefGoogle Scholar
  40. Lieb WR, Stein WD (1974b) Testing and characterising the simple carrier. Biochim Biophys Acta 373:178–196PubMedCrossRefGoogle Scholar
  41. Lieb WR, Stein WD (1976) Testing the simple carrier using irreversible inhibitors. Biochim Biophys Acta 455:913–927PubMedCrossRefGoogle Scholar
  42. Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev 41:445–502PubMedCrossRefGoogle Scholar
  43. Mitchell P (1967) Translocations through natural membranes. Adv Enzymol 29:33–88PubMedGoogle Scholar
  44. Mitchell P (1977) Vectorial chemisomotic processes. Ann Rev Biochem 46:996–1005PubMedCrossRefGoogle Scholar
  45. Murer H, Kinne R (1977) Sidedness and coupling of transport processes in small intestine and renal epithelia. In: Semenza G, Carafoli E (eds) Biochemistry of membrane transport. Springer, Berlin Heidelberg New York, pp 292–304CrossRefGoogle Scholar
  46. Murer H, Hopfer U, Kinne-Saffran E, Kinne R (1974) Glucose transport in isolated brush-border and lateral-basal plasma-membrane vesicles from intestinal epithelial cells. Biochim Biophys Acta 345:170–179PubMedCrossRefGoogle Scholar
  47. Park CR, Post RL, Kaiman CF, Wright JH Jr, Johnson LH, Morgan HE (1956) Transport of glucose and other sugars across cell membranes and the effects of insulin. Ciba Foundation Coll Endocrinol 9:240–249Google Scholar
  48. Post RL, Hegevary C, Kume S (1972) Activation by adenosine triphosphate in the phosphorylation kinetics of sodium and potassium ion transport adenosine triphosphatase. J Biol Chem 247:6530–6540PubMedGoogle Scholar
  49. Rosenberg T (1954) Concept and definition of active transport. Symp Soc Exp Biol 8:136–144Google Scholar
  50. Rosenberg T, Wilbrandt W (1957) Uphill transport induced by counterflow. J Gen Physiol 41:289–296PubMedCrossRefGoogle Scholar
  51. Schultz SF, Zalusky R (1964) Ion transport in isolated rabbit ileum II. The interaction between active sodium and active sugar transport. J Gen Physiol 47:1043–1059PubMedCrossRefGoogle Scholar
  52. Semenza G (1967) Rate equations of some cases of enzyme inhibition and activation-their application to sodium-activated membrane transport systems. J Theor Biol 15:145–176PubMedCrossRefGoogle Scholar
  53. Stein WD (1967) Movement of molecules across cell membranes. Academic, New YorkGoogle Scholar
  54. Stein WD (1977a) How the kinetic parameters of the simple carrier are affected by an applied voltage. Biochim Biophys Acta 467:376–385PubMedCrossRefGoogle Scholar
  55. Stein WD (1977b) Testing and characterising a simple carrier model for co-transport. In: Kramer M, Lauterbach F (eds) Intestinal permeation. Excerpta Medica, Amsterdam, pp 262–273Google Scholar
  56. Stein WD, Honig B (1977) Models for the active transport of cations… the steady-state analysis. Mol Cell Biochem 15:27–45PubMedCrossRefGoogle Scholar
  57. Toggenburger G, Kessler M, Rothstein A, Semenza G, Tannenbaum C (1978) Similarity in effects of Na+ gradients and membrane potentials on D-glucose transport by, and phloridzin binding to, vesicles derived from brush borders of rabbit intestinal mucosal cells. J Membrane Biol 40:269–290CrossRefGoogle Scholar
  58. Widdas WF (1952) Inability of diffusion to account for placental glucose transfer in the sheep and consideration of the kinetics of a possible carrier transfer. J Physiol (Lond) 118:23–39Google Scholar
  59. Widdas WF (1954) Facilitated transfer of hexoses across the human erythrocyte membrane. J Physiol (Lond) 125:163–180Google Scholar
  60. Wilbrandt W (1977) The asymmetry of sugar transport in the red cell membrane. In: Semenza G, Carafoli E (eds) Biochemistry of membrane transport. Springer, Berlin Heidelberg New York, pp 204–211CrossRefGoogle Scholar
  61. Wilbrandt W, Laszt LA (1933) Untersuchungen über die Ursachen der selektiven Glukoseresorption aus dem Darm. Biochem Ztschr 259:398–417Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

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  • W. D. Stein

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