On the Mechanism of Recombination Between Adenoviral and Cellular DNAs: The Structure of Junction Sites

Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 109)


The integration of adenovirus DNA into host cell DNA was studied in considerable detail in abortively and productively infected cells, as well as in transformed and tumor cells induced by human adenoviruses (for a review see Doerfler 1982). More than 70 different adenovirus-transformed cell lines and tumor cell lines from adenovirus-induced tumors exhibited non-indentical patterns of viral DNA insertion into the host genome. In this context harmster, mouse, and rat systems were investigated From these studies, there was no evidence for highly specific sites of viral DNA inserion into the cellular genome as judged by the results of Southern blotting analyses. Of course, intergratiom patterns in established transformed or tumor cell lines could have been modified by postintergrational alterations, such as rearrangements, amplifications, or partial deletions, and thus possible specificities could have been obscured. On the other hand, in several in stances it could be demonstrated that the cellular nucleotide sequences at the sites of junction were not altered (Gahlmann et al. 1982; Stabel and Doerfler 1982; Gahlmann and Doerfler? 1983). Obvious sequence specificities at the sites of insertion have so far not been found in the adenovirus, SV40, or polyoma virus system.


Adenovirus Type Junction Site Baby Hamster Kidney Cell Junction Fragment Cellular Sequence 
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© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  1. 1.Institute of GeneticsUniversity of CologneCologne 41Germany

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