Skip to main content
SpringerLink
Log in

On the Mechanism of Recombination Between Adenoviral and Cellular DNAs: The Structure of Junction Sites

  • Chapter
The Molecular Biology of Adenoviruses 1

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 109))

Abstract

The integration of adenovirus DNA into host cell DNA was studied in considerable detail in abortively and productively infected cells, as well as in transformed and tumor cells induced by human adenoviruses (for a review see Doerfler 1982). More than 70 different adenovirus-transformed cell lines and tumor cell lines from adenovirus-induced tumors exhibited non-indentical patterns of viral DNA insertion into the host genome. In this context harmster, mouse, and rat systems were investigated From these studies, there was no evidence for highly specific sites of viral DNA inserion into the cellular genome as judged by the results of Southern blotting analyses. Of course, intergratiom patterns in established transformed or tumor cell lines could have been modified by postintergrational alterations, such as rearrangements, amplifications, or partial deletions, and thus possible specificities could have been obscured. On the other hand, in several in stances it could be demonstrated that the cellular nucleotide sequences at the sites of junction were not altered (Gahlmann et al. 1982; Stabel and Doerfler 1982; Gahlmann and Doerfler? 1983). Obvious sequence specificities at the sites of insertion have so far not been found in the adenovirus, SV40, or polyoma virus system.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Benton WD, Davis RW (1977) Screening Agt recombinant clones by hybridization to single plaques in situ. Science 196: 180–182

    Article  PubMed  CAS  Google Scholar 

  • Bos JL, Polder JL, Bernards R, Schrier PI, Van den Elsen PJ, Van der Eb AJ, Van Ormondt H (1981) The 2.2 kb Elb mRNA of human Adl2 and Ad5 codes for two tumor antigens starting at different AUG triplets. Cell 27: 121–131

    Article  PubMed  CAS  Google Scholar 

  • Botchan M, Topp W, Sambrook J (1976) The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell 9: 269–287

    Article  PubMed  CAS  Google Scholar 

  • Burger H, Doerfler W (1974) Intracellular forms of adenovirus DNA. III. Integration of the DNA of adenovirus type 2 into host DNA in productively infected cells. J Virol 13: 975–992

    PubMed  CAS  Google Scholar 

  • Cook JL, Lewis AM Jr (1979) Host response to adenovirus 2–transformed hamster embryo cells. Cancer Res 39: 1455–1461

    PubMed  CAS  Google Scholar 

  • Deuring R, Doerfler W (1983) Proof or recombination between viral and cellular genomes in human KB cells productively infected by adenovirus type 12: structure of the junction site in a symmetric recombinant ( SYREC ). Gene 24

    Google Scholar 

  • Deuring R, Klotz G, Doerfler W (1981 a) An unusual symmetric recombinant between adenovirus type 12 DNA and human cell DNA. Proc Natl Acad Sci USA 78: 3142–3146

    Google Scholar 

  • Deuring R, Winterhoff U, Tamanoi F, Stabel S, Doerfler W (1981b) Site of linkage between adenovirus type 12 and cell DNAs in hamster tumor line CLAC3. Nature 293: 81–84

    Article  PubMed  CAS  Google Scholar 

  • Doerfler W (1968) The fate of the DNA of adenovirus type 12 in baby hamster kidney cells. Proc Natl Acad Sci USA 60: 636–643

    Article  PubMed  CAS  Google Scholar 

  • Doerfler W (1969) Nonproductive infection of baby hamster kidney cells (BHK21) with adenovirus type 12. Virology 38: 587–606

    Article  PubMed  CAS  Google Scholar 

  • Doerfler W (1970) Integration of the deoxyribonucleic acid of adenovirus type 12 into deoxyribonucleic acid of baby hamster kidney cells. J Virol 6: 652–666

    PubMed  CAS  Google Scholar 

  • Doerfler W (1982) Uptake, fixation and expression of foreign DNA in mammalian cells: The organization of integrated adenovirus sequences. Curr Top Microbiol Immunol 101: 127–194

    PubMed  CAS  Google Scholar 

  • Doerfler W, Lundholm U, Hirsch-Kauffmann M (1972) Intracellular forms of adenovirus deoxyribonucleic acid. I. Evidence for a deoxyribonucleic acid-protein complex in baby hamster kidney cells infected with adenovirus type 12. J Virol 9: 297–308

    PubMed  CAS  Google Scholar 

  • Doerfler W, Lundholm U, Rensing U, Philipson L (1973) Intracellular forms of adenovirus DNA. II. Isolation in dye buoyant density gradients of a DNA-RNA complex from KB cells infected with adenovirus type 2. J Virol 12: 793–807

    PubMed  CAS  Google Scholar 

  • Eick D, Doerfler W (1982) Integrated adenovirus type 12 DNA in the transformed hamster cell line T637: Sequence arrangements at the termini of viral DNA and mode of amplification. J Virol 42: 317–321

    Google Scholar 

  • Eick D, Stabel S, Doerfler W (1980) Revertants of adenovirus type 12-transformed hamster cell line T637 as tools in the analysis of integration patterns. J Virol 36: 41–49

    PubMed  CAS  Google Scholar 

  • Eick D, Kemper B, Doerfler W (1983) Excision of amplified viral DNA at palindromic sequences from the adenovirus type 12-transformed hamster cell line T637. The EMBO J 2: 1981–1986

    CAS  Google Scholar 

  • Esche H, Siegmann B (1982) Expression of early viral gene products in adenovirus type 12-infected and — transformed cells. J Gen Virol 60: 99–113

    Article  PubMed  CAS  Google Scholar 

  • Esche H, Schilling R, Doerfler W (1979) In vitro translation of adenovirus type 12-specific mRNA isolated from infected and transformed cells. J Virol 30: 21–31

    PubMed  CAS  Google Scholar 

  • Fanning E, Doerfler W (1976) Intracellular forms of adenovirus DNA. V. Viral DNA sequences in hamster cells abortively infected and transformed with human adenovirus type 12. J Virol 20: 373–383

    PubMed  CAS  Google Scholar 

  • Gahlmann R, Doerfler W (1983) Integration of viral DNA into the genome of the adenovirus type 2-transformed hamster cell line HE5 without loss or alteration of cellular nucleotides. Nucleic Acids Res 11: 7347–7361

    Article  PubMed  CAS  Google Scholar 

  • Gahlmann R, Leisten R, Vardimon L, Doerfler W (1982) Patch homologies and the integration of adenovirus DNA in mammalian cells. EMBO J 1: 1101–1104

    PubMed  CAS  Google Scholar 

  • Gallimore PH (1974) Interactions of adenovirus type 2 with rat embryo cells: Permissiveness, transformation and in vitro characteristics of adenovirus-transformed rat embryo cells. J Gen Virol 25: 263–273

    Article  PubMed  CAS  Google Scholar 

  • Gingeras TR, Sciaky D, Gelinas RE, Bing-Dong J, Yen CE, Kelly MM, Bullock PA, Parsons BL, O’Neill KE, Roberts RJ (1982) Nucleotide sequences from the adenovirus 2 genome. J Biol Chem 257: 13475–13491

    PubMed  CAS  Google Scholar 

  • Groneberg J, Doerfler W (1979) Revertants of adenovirus type 12-transformed hamster cells have lost part of the viral genomes. Int J Cancer 24: 67–74

    Article  PubMed  CAS  Google Scholar 

  • Groneberg J, Chardonnet Y, Doerfler W (1977) Integrated viral sequences in adenovirus type 12-transformed hamster cells. Cell 10: 101–111

    Article  PubMed  CAS  Google Scholar 

  • Groneberg J, Sutter D, Soboll H, Doerfler W (1978) Morphological revertants of adenovirus type 12-transformed hamster cells. J Gen Virol 40: 635–645

    Article  PubMed  CAS  Google Scholar 

  • Gutai MW, Nathans D (1978) Evolutionary variants of simian virus 40: cellular DNA sequences and sequences at recombinant joints of substituted variants. J Mol Biol 126: 275–288

    Article  PubMed  CAS  Google Scholar 

  • Hammarskjöld ML, Winberg G (1980) Encapsidation of adenovirus 16 DNA is directed by a small DNA sequence at the left end of the genome. Cell 20: 787–795

    Article  PubMed  Google Scholar 

  • Hammarskjöld ML, Winberg G (1983) Further characterization of the packaging sequence of Ad16 (Abstr). Gulbenkian Workshop on the Molecular Biology of Adenoviruses, Sintra, Portugal

    Google Scholar 

  • Hayday A, Ruley HE, Fried M (1982) Structural and biological analysis of integrated polyoma virus DNA and its adjacent host sequences cloned from transformed rat cells. J Virol 44: 67–77

    PubMed  CAS  Google Scholar 

  • Hohn B (1979) In vitro packaging of X and cosmid DNA. Methods Enzymol 68: 299–309

    Article  PubMed  CAS  Google Scholar 

  • Hohn B, Murray K (1977) Packaging recombinant DNA molecules into bacteriophage X particles in vitro. Proc Natl Acad Sci USA 74: 3259–3263

    Article  PubMed  CAS  Google Scholar 

  • Holliday R (1964) A mechanism for gene conversion in fungi. Genet Res 5: 282–304

    Article  Google Scholar 

  • Ibelgaufts H, Doerfler W, Scheidtmann KH, Wechsler W (1980) Adenovirus type 12–induced rat tumor cells of neuroepithelial origin: Persistence and expression of the viral genome. J Virol 33: 423–437

    Google Scholar 

  • Johansson K, Persson H, Lewis AM, Pettersson U, Tibbetts C, Philipson L (1978) Viral DNA sequences and gene products in hamster cells transformed by adenovirus type 2. J Virol 27: 628–639

    PubMed  CAS  Google Scholar 

  • Kemper B, Garabett M (1981) Studies on T4 head maturation. 1. Purification and characterization of gene-49-controlled endonuclease. Eur J Biochem 115: 123–131

    Article  PubMed  CAS  Google Scholar 

  • Ketner G, Kelly TJ Jr (1976) Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases. Proc Natl Acad Sci USA 73: 1102–1106

    Article  PubMed  CAS  Google Scholar 

  • Kimura T, Sawada Y, Shinawawa M, Shimizu Y, Shiroki K, Shimojo H, Sugisaki H, Takanami M, Uemizu Y, Fujinaga K (1981) Nucleotide sequence of the transforming early region Elb of adenovirus type 12 DNA: structure and gene organization, and comparison with those of adenovirus type 5 DNA. Nucleic Acids Res 9: 6571–6589

    Article  PubMed  CAS  Google Scholar 

  • Kuhlmann I, Doerfler W (1982) Shifts in the extent and patterns of DNA methylation upon explantation and subcultivation of adenovirus type 12-induced hamster tumor cells. Virology 118: 169–180

    Article  PubMed  CAS  Google Scholar 

  • Kuhlmann I, Achten S, Rudolph R, Doerfler W (1982) Tumor induction by human adenovirus type 12 in hamsters: loss of the viral genome from adenovirus type 12-induced tumor cells is compatible with tumor formation. EMBO J 1: 79–86

    PubMed  CAS  Google Scholar 

  • Kruczek I, Doerfler W (1982) The unmethylated state of the promoter/leader and 5’ regions of integrated adenovirus genes correlates with gene expression. EMBO J 1: 409–414

    PubMed  CAS  Google Scholar 

  • Landy A, Ross W (1977) Viral integration and excision: structure of the lambda att sites. Science 197: 1147–1160

    Article  PubMed  CAS  Google Scholar 

  • Majors JE, Varmus HE (1981) Nucleotide sequence at host-proviral junctions for mouse mammary tumor virus. Nature 289: 253–258

    Article  PubMed  CAS  Google Scholar 

  • Maxam AM, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74: 560–564

    Article  PubMed  CAS  Google Scholar 

  • Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base–specific chemical cleavages. Methods Enzymol 65: 499–560

    Article  PubMed  CAS  Google Scholar 

  • Mendelsohn E, Baran N, Neer A, Manor H (1982) Integration site of polyoma virus DNA in the inducible LPT line of polyoma-transformed rat cells. J Virol 41: 192–209

    PubMed  CAS  Google Scholar 

  • Mizuuchi K, Kemper B, Hays J, Weisberg RA (1982) T4 endonuclease VII cleaves Holliday structures. Cell 29: 357–365

    Article  PubMed  CAS  Google Scholar 

  • Ortin J, Scheidtmann KH, Greenberg R, Westphal M, Doerfler W (1976) Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells. J Virol 20: 355–372

    Google Scholar 

  • Roberts RJ, Sciaky D, Gelinas RE, Jiang B-D, Yen CE, Kelly MM, Bullock PA, Parsons KE, O’Neill KE, Gingeras TR (1982) Information content of adenovirus-2 genome. Cold Spring Harbor Symp Quant Biol 47: 1025–1037

    CAS  Google Scholar 

  • Robinson AJ, Younghusband HB, Bellet AJD (1973) A circular DNA-protein-complex from adenoviruses. Virology 56: 54–69

    Article  PubMed  CAS  Google Scholar 

  • Ross W, Landy A (1983) Patterns of I int recognition in the regions of strand exchange. Cell 33: 261–272

    Article  PubMed  CAS  Google Scholar 

  • Ruben M, Bacchetti S, Graham F (1983) Covalently closed circles of adenovirus 5 DNA. Nature 301: 172–174

    Article  PubMed  CAS  Google Scholar 

  • Sambrook J, Botchan M, Gallimore P, Ozanne B, Pettersson U, Williams J, Sharp PA (1974) Viral DNA sequences in cells transformed by simian virus 40, adenovirus type 2 and adenovirus type 5. Cold Spring Harbor Symp Quant Biol 39: 615–632

    Google Scholar 

  • Sambrook J, Greene R, Stringer J, Mitchison T, Hu S-L, Botchan M (1979) Analysis of the sites of integration of viral DNA sequences in rat cells transformed by adenovirus 2 or SV40. Cold Spring Harbor Symp Quant Biol 44: 569–584

    Google Scholar 

  • Sawada Y, Fujinaga K (1980) Mapping of adenovirus 12 mRNAs transcribed from the transforming region. J Virol 36: 639–651

    PubMed  CAS  Google Scholar 

  • Schick J, Baczko K, Fanning E, Groneberg J, Burger H, Doerfler W (1976) Intracellular forms of adenovirus DNA: Integrated form of adenovirus DNA appears early in productive infection. Proc Natl Acad Sci USA 73: 1043–1047

    Google Scholar 

  • Schirm S, Doerfler W (1981) Expression of viral DNA in adenovirus type 12-transformed cells, in tumor cells, and in revertants. J Virol 39: 694–702

    PubMed  CAS  Google Scholar 

  • Sharp PA, Gallimore PH, Flint SJ (1974) Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harbor Symp Quant Biol 39: 457–474

    Google Scholar 

  • Shimizu Y, Yoshida K, Ren C-S, Fujinaga K, Rajagopalan S, Chinnadurai G (1983) Hinf family: a novel repeated DNA family of the human genome. Nature 302: 587–590

    Article  PubMed  CAS  Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

    Article  PubMed  CAS  Google Scholar 

  • Stabel S, Doerfler W (1982) Nucleotide sequence at the site of junction between adenovirus type 12 DNA and repetitive hamster cell DNA in transformed cell line CLAC1. Nucleic Acids Res 10: 8007–8023

    Article  PubMed  CAS  Google Scholar 

  • Stabel S, Doerfler W, Friis RR (1980) Integration sites of adenovirus type 12 DNA in transformed hamster cells and hamster tumor cells. J Virol 36: 22–40

    PubMed  CAS  Google Scholar 

  • Stringer JR (1981) Integrated simian virus 40 DNA: Nucleotide sequences at cell-virus recombinant junctions. J Virol 38: 671–679

    PubMed  CAS  Google Scholar 

  • Stringer JR (1982) DNA sequence homology and chromosomal deletion at a site of SV40 DNA integration. Nature 296: 363–366

    Article  PubMed  CAS  Google Scholar 

  • Starzinski-Powitz A, Schulz M, Esche H, Mukai N, Doerfler W (1982) The adenovirus type 12 mouse-cell system: permissivity and analysis of integration patterns of viral DNA in tumor cells. EMBO J 1: 493–497

    PubMed  CAS  Google Scholar 

  • Sugisaki H, Sugimoto K, Takanami M, Shiroki K, Saito I, Shimojo H, Sawada Y, Uemizu Y, Uesugi S, Fujinaga K (1980) Structure and gene organization in the transforming Hindlll-G fragment of Adl2. Cell 20: 777–786

    Article  PubMed  CAS  Google Scholar 

  • Sutcliffe JG (1978) Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp Quant Biol 43: 77–90

    Google Scholar 

  • Sutter D, Doerfler W (1979) Methylation of integrated viral DNA sequences in hamster cells transformed by adenovirus 12. Cold Spring Harbor Symp Quant Biol 44: 565–568

    Google Scholar 

  • Sutter D, Doerfler W (1980) Methylation of integrated adenovirus type 12 DNA sequences in transformed cells is inversely correlated with viral gene expression. Proc Natl Acad Sci USA 77: 253–256

    Article  PubMed  CAS  Google Scholar 

  • Sutter D, Westphal M, Doerfler W (1978) Patterns of integration of viral DNA sequences in the genomes of adenovirus type 12-transformed hamster cells. Cell 14: 569–585

    Article  PubMed  CAS  Google Scholar 

  • Tikchonenko TI (to be published) Molecular biology of SI6 (SA7) and some other simian adenoviruses. In: Doerfler W (ed) The molecular biology of adenoviruses 2. Curr Top Microbiol Immunol 110

    Google Scholar 

  • Tiemeier D, Enquist L, Leder P (1976) Improved derivative of a phage X EK2 vector for cloning recombinant DNA. Nature 263: 526–527

    Article  CAS  Google Scholar 

  • Tolun A, Alestrom P, Pettersson U (1979) Sequenced of inverted terminal repetitions from different adenoviruses: demonstration of Φ conserved sequences and homology between SA 7 termini and SV40 DNA. Cell 17: 705–713

    Article  PubMed  CAS  Google Scholar 

  • Tyndall C, Younghusband HB, Bellet AJD (1978) Some adenovirus DNA is associated with the DNA of permissive cells during productive or restricted growth. J Virol 25: 1–10

    PubMed  CAS  Google Scholar 

  • Vardimon L, Doerfler W (1981) Patterns of integration of viral DNA in adenovirus type 2-transformed hamster cells. J Mol Biol 147: 227–246

    Article  PubMed  CAS  Google Scholar 

  • Visser L, Van Maarschalkerweerd MW, Rozijn TH, Wassenaar ADC, Reemst AMCB, Sussenbach JS (1979) Viral DNA sequences in adenovirus-transformed cells. Cold Spring Harbor Symp Quant Biol 44: 541–550

    Google Scholar 

  • Visser L, Reemst ACMB, Van Mansfeld ADM, Rozijn TH (1982) Nucleotide sequence analysis of the linked left and right hand terminal regions of adenovirus type 5 DNA present in the transformed rat cell line 5RK20. Nucleic Acids Res 10: 2189–2198

    Article  PubMed  CAS  Google Scholar 

  • Westin G, Visser L, Zabielski J, Van Mansfeld ADM, Pettersson U, Rozijn TH (1982) Sequence organization of a viral DNA insertion present in the adenovirus type-5-transformed hamster line BHK268-C31. Gene 17: 263–270

    Article  PubMed  CAS  Google Scholar 

  • Wilson JH, Berget PB, Pipas JM (1982) Somatic cells efficiently join unrelated DNA segments end-to-end. Mol Cell Biol 2: 1258–1269

    PubMed  CAS  Google Scholar 

  • Yasue H, Ishibashi M (1982) The oncogenicity of avian adenoviruses. III. In situ hybridization of tumor line cells localized a large number of a virocellular sequence in few chromosomes. Virology 116: 99–115

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Genetics, University of Cologne, Weyertal 121, D-5000, Cologne 41, Germany

    W. Doerfler, R. Gahlmann, S. Stabel, R. Deuring, U. Lichtenberg, M. Schulz, D. Eick & R. Leisten

Authors
  1. W. Doerfler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Gahlmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Stabel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Deuring
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. U. Lichtenberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Schulz
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. D. Eick
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. R. Leisten
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Genetik, Universität zu Köln, Weyertal 121, D-5000, Köln 41, Germany

    Walter Doerfler

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Doerfler, W. et al. (1983). On the Mechanism of Recombination Between Adenoviral and Cellular DNAs: The Structure of Junction Sites. In: Doerfler, W. (eds) The Molecular Biology of Adenoviruses 1. Current Topics in Microbiology and Immunology, vol 109. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69460-8_9

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-69460-8_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69462-2

  • Online ISBN: 978-3-642-69460-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation