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In Vitro Transcription of Adenovirus Genes

Chapter
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 109)

Abstract

Before the development of recombinant DNA technology, DNA tumor vi–ruses provided a convenient source of DNA for analysis of a limited number of genes in eukaryotic cells. The virus can be purified in large amounts and the DNA extracted from the virions is free of cellular DNA sequences. The lytic viral cycle affects cellular metabolism in a dramatic way, but does not alter the levels of cellular RNA polymerases I, II, and III or induce a virus-coded one (WEINMANN et al. 1976). The genetics of adenovirus was well developed, with deletion and temperature-sensitive mutants (For review see SHENK and WILLIAMS, Vol. ILL, (in press). The advent of restriction enzymes allowed the direct correlation of mutations with gene products and specific DNA regions. The transcribed regionds of the genome were first identified by hybridization kinetic analysis of RNA products. Combined with size analysis and hybridization to separated DNA strands, the polarities of the 3’ and 5’ ends of mRNAs were determined. At least half of the late-infected cell viral transcripts are derived from the major late promoter. A common undecanucleotide present at the 5’ end of all adenovirus structural protein mRNAs was the first suggestion for a common transcriptional start site (Gelinas and Roberts 1977).

Keywords

Ternary Complex Transcription Initiation Short RNAs Initiation Event Adenovirus Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  1. 1.The Wistar Institute of Anatomy and BiologyPhiladelphiaUSA
  2. 2.Biology DepartmentUniversity of North CarolinaChapel HillUSA

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