Skip to main content
SpringerLink
Log in
Book cover

The Molecular Biology of Adenoviruses 1 pp 107–123Cite as

The Messenger RNAs from the Transforming Region of Human Adenoviruses

  • Chapter

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 109))

Abstract

The adenoviruses are attractive to molecular biologists not because of their clinical significance, but rather as a useful tool in fundamental biological research. Both the papovaviruses and the adenoviruses have been extensively used as model systems to study the organization and expression of eukaryotic genes. Many properties unique to the eukaryotic organisms were in fact first observed in studies of animal DNA viruses and later shown to be shared by the more complex eukaryotes.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Akusjärvi G, Persson H (1981) Controls of RNA splicing and termination in the major late adenovirus transcription unit. Nature 292: 420–426

    Article  PubMed  Google Scholar 

  • Aleström P, Akusjärvi G, Perricaudet M, Mathews MB, Klessig DF, Petterson U (1980) The gene for polypeptide IX of adenovirus type 2 and its unspliced messenger RNA. Cell 19: 671–681

    Article  PubMed  Google Scholar 

  • Babich A, Nevins JR (1981) The stability of early adenovirus mRNA is controlled by the viral 72K DNA binding protein. Cell 26: 371–379

    Article  PubMed  CAS  Google Scholar 

  • Baker CC, Ziff EB (1980) Biogenesis, structures and sites encoding the 5’-termini of adenovirus-2 mRNAs. Cold Spring Harbor Symp Quant Biol 44: 415–428

    PubMed  CAS  Google Scholar 

  • Berk AJ, Sharp PA (1978) Structure of adenovirus 2 early mRNAs. Cell 14: 695–711

    Article  PubMed  CAS  Google Scholar 

  • Berk AJ, Lee F, Harrison T, Williams J, Sharp PA (1979) Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs. Cell 17: 1935–1944

    Article  Google Scholar 

  • Bos JL, Polder LJ, Bernards R, Schrier PI, van den Elsen PJ, van der Eb AJ, van Ormondt H (1981) The 2.2kb Elb mRNA of human adl2 and ad5 codes for two tumor antigens starting at different AUG triplets. Cell 27: 121–131

    Article  PubMed  CAS  Google Scholar 

  • Carlock LR, Jones N (1981) Synthesis of an unspliced cytoplasmic message by an adenovirus 5 deletion mutant. Nature 294: 572–574

    Article  PubMed  CAS  Google Scholar 

  • Chow LT, Broker TR, Lewis JB (1979) Complex splicing patterns of RNAs from the early regions of adenovirus–2. J Mol Biol 134: 265–303

    Article  PubMed  CAS  Google Scholar 

  • Colby WW, Shenk T (1981) Adenovirus type 5 virions can be assembled in vivo in the absence of detectable polypeptide IX. J Virol 39: 977–980

    PubMed  CAS  Google Scholar 

  • Dijkema R, Dekker BMM, van Ormondt H, de Waard A, Maat J, Boyer HW (1980) Gene organization of the transforming region of weakly oncogenic adenovirus type 7: the El A region. Gene 12: 287–299

    CAS  Google Scholar 

  • Dijkema R, Dekker BMM, van Ormondt H (1982) Gene organization of the transforming region of adenovirus type 7 DNA. Gene 18: 143–156

    Article  PubMed  CAS  Google Scholar 

  • Esche H, Mathews MB, Lewis JB (1980) Proteins and messenger RNAs of the transforming region of wild-type and mutant adenoviruses. J Mol Biol 142: 399–417

    Article  PubMed  CAS  Google Scholar 

  • Flint SJ, Sharp PA (1976) Adenovirus transcription. V. Quantitation of viral RNA sequences in adenovirus 2 infected and transformed cells. J Mol Biol 106: 749–771

    Article  PubMed  CAS  Google Scholar 

  • Gingeras TR, Sciaky D, Gelinas RE, Bing-Dang J, Yen C, Kelly M, Bullock P, Parsons B, O’Neill K, Roberts RJ (1982) Nucleotide sequences from the adenovirus–2 genome. J Biol Chem 257: 13475–13491

    PubMed  CAS  Google Scholar 

  • Graham FL, van der Eb AJ (1973) A new technique for assay infectivity of human adenovirus DNA. Virology 52: 456–467

    Article  PubMed  CAS  Google Scholar 

  • Graham FL, Abrahams PJ, Mulder C, Heinjeker HL, Warnaar SO, de Vries FAJ, Fiers W, van der Eb AJ (1974 a) Studies on in vitro transformation by DNA and DNA fragments of human adenoviruses and simian virus 40. Cold Spring Harbor Symp Quant Biol 39: 637–750

    Google Scholar 

  • Graham FL, van der Eb AJ, Heijneker HL (1974b) Size and location of the transforming region in human adenovirus type 5 DNA. Nature 251: 687–691

    Article  PubMed  CAS  Google Scholar 

  • Green M, Wold WSM, Brackman KH, Cartas MA (1979) Identification of families of overlapping polypeptides coded by early region 1 of human adenovirus type 2. Virology 97: 275–286

    Article  PubMed  CAS  Google Scholar 

  • Green M, Wold WSM, Brackman KH, Cartas MA (1980) Studies of early proteins and transformation proteins of human adenoviruses. Cold Spring Harbor Symp Quant Biol 44: 457–470

    PubMed  CAS  Google Scholar 

  • Halbert DN, Raskas HJ (1982) Tryptic and chymotryptic methionine peptide analysis of the in vitro translation products specified by the transforming region of adenovirus type 2. Virology 116: 406–418

    Article  PubMed  CAS  Google Scholar 

  • Halbert DN, Spector DJ, Raskas HJ (1979) In vitro translation products specified by the transforming region of adenovirus type 2. J Virol 31: 621–629

    PubMed  CAS  Google Scholar 

  • Harrison T, Graham F, Williams J (1977) Host range mutants of adenovirus type 5 defective for growth in HeLa cells. Virology 77: 319–329

    Article  PubMed  CAS  Google Scholar 

  • Harter ML, Lewis JB (1978) Adenovirus type 2 early proteins synthesized in vitro and in vivo: identification in infected cells of the 38000 to 50000-molecular-weight protein encoded by the left end of the adenovirus type 2 genome. J Virol 26: 736–749

    PubMed  CAS  Google Scholar 

  • Houweling A, van den Elsen PJ, van der Eb AJ (1980) Partial transformation of primary rat cells by the leftmost 4.5% fragment of adenovirus 5 DNA. Virology 105: 537–550

    Article  PubMed  CAS  Google Scholar 

  • Huebner RJ, Rowe WP, Lane WT (1962) Oncogenic effects in hamsters of human adenovirus type 12 and 18. Proc Natl Acad Sci USA 48: 2051–2058

    Article  PubMed  CAS  Google Scholar 

  • Jochemsen H (1981) Studies on the transforming genes and their products of human adenovirus types 12 and 5. Thesis, University of Leiden

    Google Scholar 

  • Jochemsen H, Hertoghs JJL, Lupker JH, Davis A, van der Eb A J (1981) In vitro synthesis of adenovirus type 5 T antigens. II. Translation of virus-specific RNA from cells transformed by fragments of adenovirus type 5 DNA. J Virol 37: 530–534

    PubMed  CAS  Google Scholar 

  • Jones N, Shenk T (1979) An adenovirus type 5 early gene functions regulates expression of other early viral genes. Proc Natl Acad Sci USA 76: 3665–3669

    Article  PubMed  CAS  Google Scholar 

  • Katze MB, Persson H, Philipson L (1981) Control of adenovirus early gene expression — posttranscriptional control mediated by both viral and cellular gene-products. Mol Cell Biol 9: 807–813

    Google Scholar 

  • Katze MG, Persson H, Philipson L (1982) A leftward reading transcript from the transforming region of adenovirus DNA encodes a low molecular weight polypeptide. EMBO Journal 1: 783–790

    PubMed  CAS  Google Scholar 

  • Kimura T, Sawada Y, Shinagawa M, Shimizu Y, Shiroki K, Shimojo H, Sugisaki H, Takanami M, Vemizu Y, Fujinaga K (1981) Nucleotide sequence of the transforming region of Elb of adenovirus type 12 DNA: structure and gene organization and comparison with those of adenovirus type 5 DNA. Nucleic Acids Res 9: 6571–6589

    Article  PubMed  CAS  Google Scholar 

  • Kitchingman GR, Westphal H (1980) The structure of adenovirus 2 early nuclear and cytoplasmic RNAs. J Mol Biol 137: 23–48

    Article  PubMed  CAS  Google Scholar 

  • Levinson A, Levine A J (1977) The isolation and identification of the adenovirus group C tumor antigens. Virology 76: 1–11

    Article  PubMed  CAS  Google Scholar 

  • Levinson A, Levine AJ, Anderson S, Osborn M, Rosenwirth B, Weber K (1976) The relationship between group C adenovirus tumor antigen and the adenovirus single-strand DNA binding protein. Cell 7: 575–584

    Article  PubMed  CAS  Google Scholar 

  • Lewis JB, Mathews MB (1981) Viral messenger RNAs in six lines of adenovirus-transformed cells. Virology 115: 345–360

    Article  PubMed  CAS  Google Scholar 

  • Lewis JB, Atkins JF, Baum PR, Solem R, Gesteland RB, Anderson CW (1976) Location and identification of the genes for adenovirus type 2 early polypeptides. Cell 7: 141–151

    Article  PubMed  CAS  Google Scholar 

  • Lewis JB, Esche H, Smart JE, Stilman BW, Harter ML, Mathews MB (1980) Organization and expression of the left third of the genome of adenovirus. Cold Spring Harbor Symp Quant Biol 44: 493–508

    PubMed  CAS  Google Scholar 

  • Lupker JH, Davis A, Jochemsen H, van der Eb AJ (1981) In vitro synthesis of adenovirus type 5 T antigens. I. Translation of early region 1-specific RNA from lytically infected cells. J Virol 37: 524–529

    PubMed  CAS  Google Scholar 

  • Mackey J, Wold W, Rigden P, Green M (1979) Transforming Region of Group A, B, and C Adenoviruses: DNA homology studies with twenty–nine human adenovirus serotypes. J Virol 29: 1056–1064

    Google Scholar 

  • Montell C, Fisher EF, Caruthers MH, Berk AJ (1982) Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site. Nature 295: 380–384

    Article  PubMed  CAS  Google Scholar 

  • Nevins JR (1981) Mechanism of activation of early viral transcription by the adenovirus El A gene products. Cell 26: 213–220

    Article  PubMed  CAS  Google Scholar 

  • Nevins JR, Wilson MC (1981) Regulation of adenovirus–2 gene expression at the level of transcriptional termination and RNA processing. Nature 290: 113–118

    Article  PubMed  CAS  Google Scholar 

  • Nevins JR, Ginsberg HS, Blanchard JM, Wilson MC, Darnell JE (1979) Regulation of the primary expression of early adenovirus transcription units. J Virol 32: 727–733

    PubMed  CAS  Google Scholar 

  • Perricaudet M, Akusjärvi G, Virtanen A, Pettersson U (1979) Structure of two spliced mRNAs from the transforming region of human subgroup C adenoviruses. Nature 281: 694–696

    Article  PubMed  CAS  Google Scholar 

  • Perricaudet M, Le Moullec JP, Pettersson U (1980 a) The predicted structure of two adenovirus T-antigens. Proc Natl Acad Sci USA 77: 3778–3782

    Google Scholar 

  • Perricaudet M, Le Moullec J-M, Tiollais P, Pettersson U (1980b) Structure of two adenovirus type 12 transforming polypeptides and their evolutionary implications. Nature 288:174– 176

    Google Scholar 

  • Persson H, Pettersson U, Mathews MB (1978) Synthesis of a structural adenovirus polypeptide in the absence of viral DNA replication. Virology 90: 67–79

    Article  PubMed  CAS  Google Scholar 

  • Proudfoot NJ, Brownlee GG (1976) 3’ Non-coding region sequences in eucaryotic messenger RNA. Nature 263: 211–214

    Google Scholar 

  • Ricciardi RP, Jones RL, Cepko CL, Sharp PA, Roberts BE (1981) Expression of early adenovirus genes requires a viral encoded acidic polypeptide. Proc Natl Acad Sci USA 78: 6121–6125

    Article  PubMed  CAS  Google Scholar 

  • Rowe DT, Graham FL (1981) Complementation of adenovirus type 5 host range mutants by adenovirus type 12 in coinfected HeLa and BHK–21 cells. J Virol 38: 191–197

    PubMed  CAS  Google Scholar 

  • Scolnik D (1981) An adenovirus mutant defective in splicing RNA from early region 1A. Nature 291: 508–510

    Article  Google Scholar 

  • Shaw AR, Ziff EB (1980) Transcripts from the adenovirus-2 major late promoter yield a singe family of a coterminal mRNAs during early infection and five families at late times. Cell 22: 905–916

    Article  PubMed  CAS  Google Scholar 

  • Smart JE, Lewis JB, Mathews MB, Harter ML, Andersson CW (1981) Adenovirus type 2 early proteins. Assignment of the early region 1A proteins synthesized in vivo and in vitro to specific mRNAs. Virology 112: 703–713

    Article  PubMed  CAS  Google Scholar 

  • Spector DJ, McGrogan M, Raskas HJ (1978) Regulation of the appearance of cytoplasmic RNAs from region 1 of the adenovirus genome. J Mol Biol 126: 395–414

    Article  PubMed  CAS  Google Scholar 

  • Spector DJ, Crossland LD, Halbert DN, Raskas HJ (1980) A 28K polypeptide is the translation product of 9S RNA encoded by region 1A of adenovirus 2. Virology 102: 218–221

    Article  PubMed  CAS  Google Scholar 

  • Sugisaki H, Sugimoto K, Takanami M, Shiroki K, Saito I, Shimiojio H, Sawada Y, Uemizu Y, Uesugi S-I, Fujinaga K (1980) Structure and gene organization in the transforming Hindlll-G fragment of adl2. Cell 20: 777–786

    Article  PubMed  CAS  Google Scholar 

  • Svensson C, Pettersson U, Akusjärvi G (1983) Splicing of adenovirus 2 early region 1A mRNAs is non-sequential. J Mol Biol 165: 475–479

    Article  PubMed  CAS  Google Scholar 

  • Trentin JJ, Yabe Y, Taylor G (1962) The quest for human cancer viruses. Science 137: 835–841

    Article  PubMed  CAS  Google Scholar 

  • Van den Elsen P, de Pater S, Houweling A, van der Veer I, van der Eb A (1982) The relationship between region El a and Elb of human adenoviruses in cell transformation. Gene 18: 175–185

    Google Scholar 

  • Van der Eb A, Bernards R (to be published) Transformation and oncogenecity by adenoviruses. In: Doerfler W (ed) The molecular biology of adenoviruses 2. Curr Top Microbiol Immunol 110

    Google Scholar 

  • Van der Eb AJ, Mulder C, Graham FL, Houweling A (1977) Transformation with specific fragments of adenovirus DNAs. I. Isolation of specific fragment with transforming activity of adenovirus 2 and 5 DNA. Gene 1: 115–132

    Google Scholar 

  • Van der Eb AJ, van Ormondt H, Schrier PI, Lupker JH, Jochemsen H, van den Elsen PJ, de Leys RJ, Maat J, van Beveren CP, Dijkema R, de Waard A (1979) Structure and function of the transforming genes of human adenoviruses and SV40. Cold Spring Harbor Symp Quant Biol 44: 383–399

    Google Scholar 

  • Van Ormondt H, Hesper B (1983) Comparison of the nucleotide sequences of early region Elb DNA of human adenovirus types 12, 7 and 5 (subgroups A, B and C) Gene 21: 217–226

    Google Scholar 

  • Van Ormondt H, Maat J, Dijkema R ( 1980 a) Comparison of nucleotide sequences of the early El a regions for subgroups A, B and C of human adenviruses. Gene 12: 63–76

    Google Scholar 

  • Van Ormondt H, Maat J, Van Beveren CP (1980 b) The nucleotide sequence of the transforming region El of adenovirus type 5 DNA. Gene 11: 299–399

    Google Scholar 

  • Virtanen A, Pettersson U (1983) The molecular structure of the 9S mRNA from early region 1A of adenovirus 2. J Mol Biol 165: 496–499

    Article  PubMed  CAS  Google Scholar 

  • Virtanen A, Pettersson U, LeMoullec JM, Tiollais P, Perricaudet M (1982) Different mRNA structures are transcribed from the transforming region of highly and non-oncogenic human adenoviruses. Nature 295: 705–707

    Article  PubMed  CAS  Google Scholar 

  • Wilson MC, Darnell JE Jr (1981) Control of mRNA concentration by differential cytoplasmic half-life: adenovirus mRNAs from transcription units 1A and IB. J Mol Biol 148: 231–251

    Article  PubMed  CAS  Google Scholar 

  • Wilson MC, Fraser N, Darnell J (1979 a) Mapping of RNA initiation sites by high doses of UV irradiation. Evidence for three independent promoters within the left 11% of the Ad-2 genome. Virology 94: 175–184

    Google Scholar 

  • Wilson MC, Nevins JR, Blanchard JM, Ginsberg HS, Darnell JE Jr (1979 b) The metabolism of mRNA from the transforming region of adenovirus type 2. Cold Spring Harbor Symp Quant Biol 44: 447–455

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Medical Genetics and Microbiology, The Biomedical Center, Box 589, S-751 23, Uppsala, Sweden

    Ulf Pettersson, Anders Virtanen & Göran Akusjärvi

  2. Institut de Recherches, Scientifiques sur le Cancer, Rue Guy-Mocquet, F-94800, Villejuif, France

    Michel Perricaudet

Authors
  1. Ulf Pettersson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anders Virtanen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michel Perricaudet
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Göran Akusjärvi
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institut für Genetik, Universität zu Köln, Weyertal 121, D-5000, Köln 41, Germany

    Walter Doerfler

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Pettersson, U., Virtanen, A., Perricaudet, M., Akusjärvi, G. (1983). The Messenger RNAs from the Transforming Region of Human Adenoviruses. In: Doerfler, W. (eds) The Molecular Biology of Adenoviruses 1. Current Topics in Microbiology and Immunology, vol 109. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69460-8_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-69460-8_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69462-2

  • Online ISBN: 978-3-642-69460-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation