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A New Mechanism for the Initiation of Replication of Φ29 and Adenovirus DNA:Priming by the Terminal Protein

  • Chapter
The Molecular Biology of Adenoviruses 1

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 109))

Abstract

Bacteriophage Φ29 fromBacillus subtiliscontains a double-stranded DNA of molecular weight 11.8 x 106(SOGO et al. 1979) that can be isolated from the viral particles as a circular molecule closed noncovalently by protein (ORTÌN etal. 1971). In this review I will describe our present knowledge of the protein linked to 029 DNA. I will present first the structural aspects of the problem: characterization of the protein and the nature of the protein-DNA linkage and sequence at the terminals of Φ29 DNA and of the gene coding for the terminal protein. Next I will describe the mechanism of Φ29 DNA replication and how the terminal protein functions as a “primer” in the initiation of replication. Other requirements for the initiation reaction will be also described, as well as the production of specific mutations at the carboxyl end of the terminal protein. Finally, I will compare the Φ29 and adenovirus DNA-protein complexes and the novel mechanism used for the initiation of replication in the two systems.

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Authors and Affiliations

  1. Centro de Biologia Molecular (CSIC-UAM), Universidad Autönoma, Canto Blanco, E-Madrid-34, Mexico

    Margarita Salas

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  1. Margarita Salas
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Editors and Affiliations

  1. Institut für Genetik, Universität zu Köln, Weyertal 121, D-5000, Köln 41, Germany

    Walter Doerfler

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© 1983 Springer-Verlag Berlin Heidelberg

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Salas, M. (1983). A New Mechanism for the Initiation of Replication of Φ29 and Adenovirus DNA:Priming by the Terminal Protein. In: Doerfler, W. (eds) The Molecular Biology of Adenoviruses 1. Current Topics in Microbiology and Immunology, vol 109. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69460-8_4

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  • DOI: https://doi.org/10.1007/978-3-642-69460-8_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69462-2

  • Online ISBN: 978-3-642-69460-8

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