A New Mechanism for the Initiation of Replication of Φ29 and Adenovirus DNA:Priming by the Terminal Protein
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Bacteriophage Φ29 fromBacillus subtiliscontains a double-stranded DNA of molecular weight 11.8 x 106(SOGO et al. 1979) that can be isolated from the viral particles as a circular molecule closed noncovalently by protein (ORTÌN etal. 1971). In this review I will describe our present knowledge of the protein linked to 029 DNA. I will present first the structural aspects of the problem: characterization of the protein and the nature of the protein-DNA linkage and sequence at the terminals of Φ29 DNA and of the gene coding for the terminal protein. Next I will describe the mechanism of Φ29 DNA replication and how the terminal protein functions as a “primer” in the initiation of replication. Other requirements for the initiation reaction will be also described, as well as the production of specific mutations at the carboxyl end of the terminal protein. Finally, I will compare the Φ29 and adenovirus DNA-protein complexes and the novel mechanism used for the initiation of replication in the two systems.
KeywordsInitiation Complex Initiation Reaction Covalent Complex Terminal Protein Bacillus Subtilis Phage
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- Carrascosa JL, Camacho A, Moreno F, Jimenez F, Mellado RP, Viñuela E, Salas M (1976)Bacillus subtilisphage Φ 29: characterization of gene products and functions. Eur J Biochem 66: 229–241Google Scholar
- García J A, Pastrana R, Prieto I, Salas M (1983 a) Cloning and expression inEscherichia coliof the gene coding for the protein linked to the ends ofBacillus subtilisphage Φ 29 DNA. Gene 21: 65–76Google Scholar
- García E, Gómez A, Ronda C, Escarmís C, López R (1983 b) Pneumococcal bacteriophage Cp-1 contains a protein tightly bound to the 5’ termini of its DNA. Virology 128: 92–104Google Scholar
- García J A, Peñalva MA, Blanco L, Salas M, Template requirements for the initiation of phage Φ 29 DNA replication in vitro. Proc Natl Acad Sci USA, in pressGoogle Scholar
- Mellado RP, Salas M. Initiation of phage Φ 29 DNA replication by the terminal protein modified at the carboxyl end. Nucleic Acids Res, in pressGoogle Scholar
- Salas M, García JA, Peñalva MA, Blanco L, Prieto I, Mellado RP, Lazaro JM, Pastrana R, Escarmis C, Hermoso JM (1983) Requirements for the initiation of phage Φ 29 DNA replication in vitro primed by the terminal protein. In: Cozzarelli NR (ed) Mechanisms of DNA replication and recombination. Liss, New York (UCLA symposia on molecular and cellular biology, new series, vol 10.)Google Scholar
- Smart JE, Stillman BW (1982) Adenovirus terminal protein precursor. Partial amino acid sequence and the site of covalent linkage to virus DNA. J Biol Chem 257: 13499–13506Google Scholar