The Mechanism of Adenovirus DNA Replication and the Characterization of Replication Proteins
The replication of the genome of human adenoviruses in permissive host cells is a highly efficient process. During the infection cycle a total amount of viral DNA is synthesized comparable to the entire chromosomal DNA content of the infected cell. In order to unravel the mechanism of adenovirus DNA replication this process has been studied in a number of experimental systems.
KeywordsAdenovirus Type Replicative Intermediate Late Gene Expression Infected Nucleus Complementary Strand Synthesis
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- Conaway RC, Lehman IR (1982) Synthesis by the DNA primase of Drosophila Melanogaster of a primer with a unique chain length. Proc Natl Acad Sci USA 79: 4584–4588Google Scholar
- Coombs DH, Robinson AJ, Bodnar JW, James CJ, Pearson GD (1978) Detection of DNA- protein complexes: the adenovirus DNA-terminal protein and HeLa DNA protein complexes. Cold Spring Harbor Symp Quant Biol 43: 741–753Google Scholar
- Ginsberg HS, Ensinger MJ, Kauffman RS, Mayer AJ, Lundholm U (1974) Cell transformation: a study of regulation with types 5 and 12 adenovirus temperature-sensitive mutants. Cold Spring Harbor Symp Quant Biol 39: 419–426Google Scholar
- Kelly TJ, Lechner RL (1978) The structure of replicating adenovirus DNA molecules: characterization of DNA-protein complexes from infected cells. Cold Spring Harbor Symp Quant Biol 43: 721–728Google Scholar
- Rijnders AWM, van Bergen BGM, van der Vliet PC, Sussenbach JS (to be published) Immunological characterization of the role of adenovirus terminalprotein in viral DNA replication. VirologyGoogle Scholar
- Sussenbach JS, van der Vliet PC, Ellens DJ, Jansz HS (1972) Linear intermediates in the replication of adenovirus DNA. Nature 239: 47–49Google Scholar
- Van Bergen BGM, van der Vliet PC (1983) Temperature-sensitive initiation and elongation of adenovirus DNA replication in vitro with nuclear extracts from H5ts36, H5ts149 and H5ts125 infected HeLa cells. J Virol 42: 642–648Google Scholar
- Van der Vliet PC, Levine AJ (1973) DNA binding proteins specific for cells infected by adenoviruses. Nature 246: 1709–174Google Scholar
- Van der Vliet, Levine AJ, Ensinger M, Ginsbergs HS (1975) Thermolabile DNA binding proteins from cells infected with a temperature-sensitive mutant of adenovirus defective in viral DNA synthesis. J Virol 15: 348–354Google Scholar
- Wilhelm J, Brison O, Kedinger C, Chambon P (1976) Characterization of adenovirus type 2 transcriptional complexes isolated from infected HeLa cell nuclei. J Virol 15: 744–758Google Scholar