Flow Cytometric Quantification of Radiation Responses of Murine Peritoneal Cells
Methods have been developed to distinguish subpopulations of murine peritoneal cells, and these were applied to the measurement of early changes in peritoneal cells after irradiation. The ratio of the two major subpopulations in the peritoneal fluid, lymphocytes and macrophages, was measured rapidly by means of cell volume distribution analysis as well as by hypotonic propidium iodide (PI) staining. After irradiation, dose and time dependent changes were noted in the cell volume distributions: a rapid loss of peritoneal lymphocytes, and an increase in the mean cell volume of macrophages. The hypotonic PI staining characteristics of the peritoneal cells showed two or three distinctive G1 peaks. The ratio of the areas of these peaks was also found to be dependent on the radiation dose and the time after irradiation. These results demonstrate that these two parameters may be used to monitor changes induced by irradiation (biological dosimetry), and to sort different peritoneal subpopulations. @@@ The work was suported by Grant CA 22585 from the National Cancer Institute, U.S. DHEW and by the U.S. Department of Energy.
KeywordsPropidium Iodide Radiation Response Peritoneal Cell Propidium Iodide Solution Fluorescence Intensity Peak
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