Abstract
Genetic recombination is the fundamental biological process of exchange of genetic information between different chromosomes. Its constant activity during evolution has enabled the large number of spontaneously occurring genetic changes that improve the fitness of individuals for their particular environments to accumulate in the most successful members of each biological species. Recombination has provided much of the experimental basis for the science of genetics and has been exploited to great social benefit by plant and animal breeders and to great scientific benefit by classical and molecular geneticists.
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References
Cohen SN (1975) The manipulation of genes. Sci Am 233: 24–35
Setlow JK, Hollaender A (eds) (1980) Genetic engineering, principles and methods, vol 2. Plenum Press, New York
Timmis KN (1981) Gene manipulation in vitro. In: Glover SW, Hopwood DA (eds) Soc Gen Microbiol Symp 31, Genetics as a tool in microbiology. Cambridge Univ Press, Cambridge, pp 49–109
Wu R (ed) (1979) Methods in enzymology, vol 68. Recombinant DNA. Academic Press, New York
Cohen SN (1975) The manipulation of genes. Sci Am 233: 24–35
Setlow JK, Hollaender A (eds) (1980) Genetic engineering, principles and methods, vol 2. Plenum Press, New York
Timmis KN (1981) Gene manipulation in vitro. In: Glover SW, Hopwood DA (eds) Soc Gen Microbiol Symp 31, Genetics as a tool in microbiology. Cambridge Univ Press, Cambridge, pp 49–109
Wu R (ed) (1979) Methods in enzymology, vol 68. Recombinant DNA. Academic Press, New York
Dugaiczyk A, Boyer HW, Goodman HM (1975) Ligation of EcoRl endonuclease-generated DNA fragments into linear and circular structures. J Mol Biol 96: 171–184
Sgaramella V, van de Sande JH, Khorana HG (1970) Studies on polynucleotides. C. A novel joining reaction catalyzed by the T4–polynucleotide ligase. Proc Natl Acad Sci USA 67: 1468–1475
Franklin FCH, Bagdasarian M, Bagdasarian MM, Timmis KN (1981) Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway. Proc Natl Acad Sei USA 78: 7458–7462
Hohn B, Hinnen A (1980) Cloning with cosmids in E. coli and yeast. In: Setlow JK, Hollaender A (eds) Genetic engineering, principles and methods, vol 2. Plenum Press, New York, pp 169–183
Lehrbach PR, Timmis KN (1983) Genetic analysis and manipulation of catabolic pathways in Pseudomonas. Biochem Soc Symp 48: 191–219
Long SR, Buikema WJ, Ausubel FM (1982) Cloning of Rhizobium meliloti nodulation genes by direct complementation of Nod mutants. Nature 298: 485–488
Ozaki LS, Maeda S, Shimada K, Takagi Y (1980) A novel ColEl:TnJ plasmid vector that allows direct selection of hybrid clones in E. coli. Gene 8: 301–314
Roychoudhury R, Jay E, Wu R (1976) Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase. Nucleic Acids Res 3: 101–116
Schumann W (1979) Construction of an Hpal and Hindll plasmid vector allowing direct selection of transformants harbouring recombinant plasmids. Mol Gen Genet 174: 221–224
Setlow JK, Hollaender A (eds) (1980) Genetic engineering, principles and methods. Plenum Press, New York
Timmis KN (1981) Gene manipulation in vitro. In: Glover SW, Hopwood DA (eds) Soc Gen Microbiol Symp 31. Genetics a a tool in microbiology. University Press, Cambridge, pp 49–109
Timmis KN, Cabello F, Cohen SN (1974) Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons. Proc Natl Acad Sci USA 71: 4556–4560
Timmis KN, Cabello F, Cohen SN (1978) Cloning and characterization of EcoRI and Hindlll restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol Gen Genet 162: 121–137
Ullrich A, Shine J, Chirgwin J, Pictet R, Tischer E, Rutter WJ, Goodman HM (1977) Rat insulin gene: construction of plasmids containing the coding sequences. Science 196: 1313–1319
Bagdasarian M, Bagdasarian MM, Lurz R, Nordheim A, Frey J, Timmis KN (1982) Molecular and functional analysis of the broad host range plasmid RSF1010 and construction of vectors for gene cloning in Gram negative bacteria. In: Mitsuhashi S (ed) Resistance in bacteria: genetics, biochemistry and molecular biology. Japan Sei Soc Press, Tokyo
Kornberg A (1980) DNA replication. Freeman, San Francisco
Kornberg A (1982) 1982 Supplement to DNA replication. Freeman, San Francisco
Lovett MA, Helinski DR (1976) Method for the isolation of the replication region of a bacterial replicon: construction of a mini–F Km plasmid. J Bacteriol 127: 982–987
Messer W, Bergmans HEN, Meijer M, Womack JE, Hansen FG, von Meyenberg K (1978) Minichromosomes: plasmids which carry the E. coli replication origin. Mol Gen Genet 162:269– 75
Thomas CM, Helinski DR (1979) Plasmid DNA replication. In: Timmis KN, Pühler A (eds) Plasmids of medical, environmental and commercial importance. Elsevier/North-Holland, Amsterdam, pp 29–46
Timmis KN, Cabello F, Cohen SN (1975) Cloning, isolation and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sei USA 72: 2242–2246
Timmis KN, Andres I, Slocombe PM (1978) Plasmid incompatibility: cloning analysis of an IncFII determinant of R6-5. Nature 273: 27–32
Timmis KN, Danbara H, Brady G, Lurz R (1980) Inheritance functions of group IncFII transmissible antibiotic resistance plasmids. Plasmid 5: 53–75
Yasuda S, Hirota Y (1977) Cloning and mapping of the replication origin of Escherichia coll Proc Natl Acad Sei USA 74: 5458–5462
Blattner FR, Williams BG, Blechl AE, Denniston–Thompson K, Faber HE, Fulong LA, Grunwald DJ, Moore DD, Schümm JW, Sheldon EL, Smithies O (1977) Charon phages: safe derivatives of bacteriophage lambda for DNA cloning. Science 196: 161–169
Hershey AD (1971) The bacteriophage lambda. Gen Res Unit, Carnegie Inst, Cold Spring Harbor Lab, Cold Spring Harbor New York
Murray NE, Brammar WJ, Murray K (1977) Lambdoid phages that simplify the recovery of in Collins J, Hohn B (1978) Cosmids: A type of plasmid gene-cloning vector that is packable in vitro in bacteriophage X heads. Proc Natl Acad Sei USA 75: 4242–4246
Feiss M, Siegele DA (1979) Packaging of the bacteriophage X chromosome: dependence of cos cleavage on chromosome length. Virology 92: 190–200
Feiss M, Fisher RA, Crayton MA, Egner C (1977) Packaging of the bacteriophage X chromosome: effect of chromosome length. Virology 77: 281–293
Hohn B (1979) In vitro packaging of X and cosmid DNA. Methods Enzymol 68: 299–309
Hohn B, Collins J (1980) A small cosmid for efficient cloning of large DNA fragments. Gene 11: 291–298
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Lab, Cold Spring Harbor New York
Meade HM, Long SR, Ruvkun GB, Brown SE, Ausubel FM (1982) Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J Bacteriol 149: 114–122
Sternberg N, Tiemeier D, Enquist L (1977) In vitro packaging of a X dam vector containing ifcoRI DNA fragments of Escherichia coli and phage PI, Gene 1: 255–280
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Timmis, K.N., Brady, G., Frey, J., Pühler, A., Priefer, U., Simon, R. (1984). Gene Cloning. In: Pühler, A., Timmis, K.N. (eds) Advanced Molecular Genetics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69305-2_3
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DOI: https://doi.org/10.1007/978-3-642-69305-2_3
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