Growth-Promoting Activity of Thrombin in Fibroblasts: Temporal Action and Early Biochemical Events in the G0 → G1 Transition

  • J. Pouysségur
  • J. C. Chambard
  • A. Franchi
  • G. L’Allemain
  • S. Paris
  • E. Van Obberghen-Schilling
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Polypeptide growth factors and hormones have been shown to play an essential role in the regulation of cell proliferation and cell differentiation. However, the molecular mechanisms by which these extracellular signals act on quiescent cells to induce the G0→G1 transition, DNA replication and subsequent cell division or differentiation are unknown. The introduction by Sato and coworkers of cell culture in serum-free hormone-supplemented medium (1) has greatly facilitated the experimental analysis of the mechanisms of growth factor action. The addition of purified growth-promoting polypeptides to G0-arrested fibroblasts induces a multiple array of biochemical events leading to activation of protein synthesis and subsequent DNA synthesis. Changes in ionic membrane permeability and in protein phosphorylation occur rapidly after stimulation (2–8). These changes, which appear to be ubiquitous, raise the central question of how distinct growth factor receptors translate the variety of external signals into a common activation of cellular metabolism and cell proliferation. The purpose of this paper is to present the growth-promoting activity of α-thrombin in serum-free conditions We will analyze the temporal action of thrombin during the G0→G1 transition of the cell cycle and the post-receptor, early-stimulated biochemical events: activation of a Na+/H+ exchange and stimulation of protein phosphorylation.

Keywords

Boron Polypeptide Thrombin Neuroblastoma Thymidine 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • J. Pouysségur
    • 1
  • J. C. Chambard
    • 1
  • A. Franchi
    • 1
  • G. L’Allemain
    • 1
  • S. Paris
    • 1
  • E. Van Obberghen-Schilling
    • 1
  1. 1.Centre de Biochemie, CNRSUniversité de NiceNiceFrance

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