3T3 Cell Growth Initiation and Multiplication in Defined Medium Supplemented with Growth Factors and Hormones That Replace Serum
Most mammalian cells in culture do not grow in basal tissue culture medium unless serum is added as medium supplement.It is clear that several growth factors present in serum support 3T3 cell prolife-ration (1,2). Using swiss 3T3 mouse cells cultured in a suboptimal culture medium (DME) this laboratory has been involved for several years in characterizing serum growth factors in attempts to use them to substitute for serum in order to grow 3T3 cells in chemically defined medium (3). Other laboratories have shown that by optimizing the composition of culture media the amount of serum protein required for clonal growth of various cell types can drastically be reduced (4,5). Many cell types, most notably of transformed phenotype, can be cultured successfully in serum-free medium supplemented with hormones and growth factors (9). However, extensive work during the last few years aimed at culturing 3T3 cells in defined medium has suggested that in addition to hormones such as insulin, hydrocortisone and growth factors such as Fibroblast Growth Factor (FGF) (6), Epidermal Growth Factor (EGF) (7) or Platelet-derived Growth Factor (PDGF) (8) additional still unknown activities in serum are necessary to replace serum growth promoting activity (10–12).
KeywordsFibroblast Growth Factor Clonal Growth SV3T3 Cell General Culture Condition Replace Serum
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