Continuous Growth of a Human Breast Cancer Cell Line (MCF-7) in Serum-Free Medium
The MCF-7 cell line has been adapted to growth in serum-free medium by a gradual decrease in the content of serum in the medium. The serum-free medium was composed of DMEM/F12 + glutamine + selenite + insulin + transferrin + EGF, and the culture flasks were coated with collagen IV to increase cell attachment. The doubling time for cells cultivated in serum-free medium for 3 months was 70 hrs. The cells contained free cytoplasmic but not filled nuclear estrogen receptors and formed carcinomas when inoculated subcutaneously into nude mice treated with estrone.
KeywordsEstrogen EDTA Glutamine Trypsin Cytosol
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