Continuous Growth of a Human Breast Cancer Cell Line (MCF-7) in Serum-Free Medium

  • P. Briand
  • A. E. Lykkesfeldt
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


The MCF-7 cell line has been adapted to growth in serum-free medium by a gradual decrease in the content of serum in the medium. The serum-free medium was composed of DMEM/F12 + glutamine + selenite + insulin + transferrin + EGF, and the culture flasks were coated with collagen IV to increase cell attachment. The doubling time for cells cultivated in serum-free medium for 3 months was 70 hrs. The cells contained free cytoplasmic but not filled nuclear estrogen receptors and formed carcinomas when inoculated subcutaneously into nude mice treated with estrone.


Estrogen Receptor Human Breast Cancer Cell Collagen Coating Microwell Plate Silicone Grease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Barnes D and Sato G: Nature 1979, 281: 388–389.PubMedCrossRefGoogle Scholar
  2. 2.
    Schor SL and Court J: J Cell Sci 1979, 38: 267–281.PubMedGoogle Scholar
  3. 3.
    Garola RE and McGuire WL: Cancer Res 1977, 37: 3333–3337.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • P. Briand
    • 1
  • A. E. Lykkesfeldt
    • 1
  1. 1.The Fibiger LaboratoryCopenhagenDenmark

Personalised recommendations