Secretion of α-MSH-Like Molecules in a Human Melanoma Cell Line

  • N. Van Tieghem
  • M. Fooij
  • P.-E. Henry
  • F. Legros
  • J.-M. Prevost
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Receptors for exogenous 125I-α-MSH were detected, at passage 54, on the human melanoma 809 cell line, using a specific hormone receptor assay. Thereafter (at pass. 80–90], receptors were consistently absent. To test the hypothetic blockade by MSH-like molecules, secreted by the non receptive cells themselves, a protein A immunoassay was devised to detect the presence of cross-reacting polypeptides on the cell surface. When receptivity for the hormone disappeared, α-MSH immunoreactivity was present at the cell surface. No re over, hormone receptive cells (at pass. <60) were able to interrant with serum-free conditioned medium obtained from non receptive cells (at pass. 94). These results suggest that 809 melanoma cells bear on their surface α-MSH-like peptides, which are recognized by highly specific α-MSH antibodies, and release into supernatant medium α-MSH-like molecules, able to interact with specific α-MSH receptors.

Keywords

Polypeptide Staphylococcus 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • N. Van Tieghem
    • 1
  • M. Fooij
    • 1
  • P.-E. Henry
    • 1
  • F. Legros
    • 1
  • J.-M. Prevost
    • 1
  1. 1.Faculté de MédecineUniversité Libre de BruxellesBelgium

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