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Secretion of α-MSH-Like Molecules in a Human Melanoma Cell Line

  • N. Van Tieghem
  • M. Fooij
  • P.-E. Henry
  • F. Legros
  • J.-M. Prevost
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Receptors for exogenous 125I-α-MSH were detected, at passage 54, on the human melanoma 809 cell line, using a specific hormone receptor assay. Thereafter (at pass. 80–90], receptors were consistently absent. To test the hypothetic blockade by MSH-like molecules, secreted by the non receptive cells themselves, a protein A immunoassay was devised to detect the presence of cross-reacting polypeptides on the cell surface. When receptivity for the hormone disappeared, α-MSH immunoreactivity was present at the cell surface. No re over, hormone receptive cells (at pass. <60) were able to interrant with serum-free conditioned medium obtained from non receptive cells (at pass. 94). These results suggest that 809 melanoma cells bear on their surface α-MSH-like peptides, which are recognized by highly specific α-MSH antibodies, and release into supernatant medium α-MSH-like molecules, able to interact with specific α-MSH receptors.

Keywords

Melanoma Cell Melanoma Cell Line Human Melanoma Cell Human Melanoma Cell Line Supernatant Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • N. Van Tieghem
    • 1
  • M. Fooij
    • 1
  • P.-E. Henry
    • 1
  • F. Legros
    • 1
  • J.-M. Prevost
    • 1
  1. 1.Faculté de MédecineUniversité Libre de BruxellesBelgium

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