Serum-Free Cultures of Mouse LM Fibroblasts as a Tool for Biochemical and Genetic Studies in the Cellular Responses to Interferon

  • P. Milhaud
  • T. Faure
  • J. Chaintreuil
  • B. Lebleu
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


LM cells continuously growing in the absence of serum and exogenous lipids have been used for studies on the mechanism of action of interferon (IFN). Several of its biological activities, namely the inhibition of virus and cell growth and the increased cytotoxicity to Poly(rI).Poly(rC) (=dsRNA), are shown to be increased in such conditions. None of these three effects of IFN depends on an active prostaglandin biosynthesis, as previously proposed, since cells in our conditions are lacking the necessary precursors.

Variants have been obtained which are resistant to the increased cytotoxicity of IFN-treated cells to Poly(rI).Poly(rC). We believe that this model will be useful for studies on the mechanism of action of IFN through the isolation of variants and the analysis of lipidic and proteic requirements of its activity.


Antiviral Response Glass Capillary Column Exogenous Lipid Active Prosta Rapid Cell Lysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aguet (1980) Nature, 284, 459–461.PubMedCrossRefGoogle Scholar
  2. Burke (1977) Trends Biochem. Sci., Nov, 249–251.Google Scholar
  3. Chandrabose et al. (1981) Science, 212, 329–331.PubMedCrossRefGoogle Scholar
  4. Chang and Vagelos (1976) Proc. Natl. Acad. Sci. USA, 73, 24–28.PubMedCrossRefGoogle Scholar
  5. Gresser et al. (1970) J. Natl. Cancer Inst., 45, 1145–1153.PubMedGoogle Scholar
  6. Kading et al. (1978) Arch. Virol., 56, 237–2477PubMedCrossRefGoogle Scholar
  7. Pottathil et al. (1980) Proc. Natl. Acad. Sci. USA, 77, 5437–5440.PubMedCrossRefGoogle Scholar
  8. Wallach and Revel (1979) FEBS Lett., 101, 364–368.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • P. Milhaud
    • 1
  • T. Faure
    • 2
  • J. Chaintreuil
    • 3
  • B. Lebleu
    • 2
  1. 1.Laboratoire de Biologie Moleculaire, ERA CNRS 482Universite de Montpellier IIMontpellierFrance
  2. 2.Laboratoire de Biochimie des Proteines, ERA CNRS 482Universite de Montpellier IIMontpellierFrance
  3. 3.Laboratoire de BiochimieUniversite de Montpellier IMontpellierFrance

Personalised recommendations