The Role of Fetal Calf Serum (FCS) During the First Stages of Hepatocytes Cultures
Fibronectin (FN) from serum of unrelated vertebrates have the same ability as FCS to allow hepatocyte attachment and spreading.FN mediated attachment is an active process selective for viable cells that shous sigmoid kinetics,strong dependence of temperature and divalent cations. Tire presence of glucagon and albumin in culture was found to be important environmental requirements for complete hepatocyte spreading that involves RNA,protein synthesis and cytoskeleton rearrangement.
KeywordsCellulose Albumin Dexamethasone Polystyrene Glucagon
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