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The Role of Fetal Calf Serum (FCS) During the First Stages of Hepatocytes Cultures

  • M. J. Gómez-Lechón
  • J. V. Castell
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

Fibronectin (FN) from serum of unrelated vertebrates have the same ability as FCS to allow hepatocyte attachment and spreading.FN mediated attachment is an active process selective for viable cells that shous sigmoid kinetics,strong dependence of temperature and divalent cations. Tire presence of glucagon and albumin in culture was found to be important environmental requirements for complete hepatocyte spreading that involves RNA,protein synthesis and cytoskeleton rearrangement.

Keywords

Fetal Calf Serum Cell Attachment Environmental Requirement Hepatocyte Culture Cytoskeleton Rearrangement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • M. J. Gómez-Lechón
    • 1
  • J. V. Castell
    • 1
  1. 1.Centro de InvestigaciónCiudad Sanitaria La FeValencia-9Spain

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