Hybridoma Formation in Serum-Free Medium
The production of Ig-secreting hybridomas from fusions between mouse myeloma cells and mouse immunized spleen cells in serum-free medium has several practical advantages in monoclonal antibody research in terms of simplification of the process of preliminary screening for biological activities of the monoclonal antibodies as well as their purifications from the culture supernatants (1–3). Several attempts in applying serum-free media which have been developed specifically for the growth of established NS-1 myeloma derived hybridomas (4,5) in the isolation of nascent NS-1 meyloma-derived hybridomas have been unsuccessful (Kawamoto et al, in preparation). This failure leads us to focus our attentions on the conditions which would support the growth of the parent myeloma cell lines, NS-1, which as we anticipated, would also promote the formation and the outgrowth of their derived hybridomas.
KeywordsOleic Acid Myeloma Cell Clonal Cell Line Bovine Serum Albumin Complex Parent Myeloma
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