Advertisement

Hybridoma Formation in Serum-Free Medium

  • Tomoyuki Kawamoto
  • J. Denry Sato
  • Anh Le
  • Don B. McClure
  • Gordon H. Sato
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

The production of Ig-secreting hybridomas from fusions between mouse myeloma cells and mouse immunized spleen cells in serum-free medium has several practical advantages in monoclonal antibody research in terms of simplification of the process of preliminary screening for biological activities of the monoclonal antibodies as well as their purifications from the culture supernatants (1–3). Several attempts in applying serum-free media which have been developed specifically for the growth of established NS-1 myeloma derived hybridomas (4,5) in the isolation of nascent NS-1 meyloma-derived hybridomas have been unsuccessful (Kawamoto et al, in preparation). This failure leads us to focus our attentions on the conditions which would support the growth of the parent myeloma cell lines, NS-1, which as we anticipated, would also promote the formation and the outgrowth of their derived hybridomas.

Keywords

Oleic Acid Myeloma Cell Clonal Cell Line Bovine Serum Albumin Complex Parent Myeloma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Sato, G. H. (1975) in Biochemical Actions of Hormones, Vol. III, pp. 391–396, ( G. Litwack, ed.), Academic Press, New York.Google Scholar
  2. 2.
    Barnes, D., and Sato, G. H. (1980a) Anal, biochem. 102: 255–270.CrossRefGoogle Scholar
  3. 3.
    Barnes, D., and Sato, G. H. (1980b) Cell 22: 649–655.PubMedCrossRefGoogle Scholar
  4. 4.
    Chang, T. H., Steplewski, Z., and Koprowski, H. (1980) J. Immunol. Methods 39: 369–375.PubMedCrossRefGoogle Scholar
  5. 5.
    Murakami, H., Masui, H., Sato, G. H., Sueoka, N., Chow, T. P. and Kano-Sueoka, T. (1982) Proc. Natl. Acad. Sci. USA 79: 1158–1162.PubMedCrossRefGoogle Scholar
  6. 6.
    Wolfei R., Sato, G. H. and McClure, D. B. (1980) J. Cell Biol. 87: 434–441.CrossRefGoogle Scholar
  7. 7.
    Galfre, G., Howe, S. C., Molstein, C., Butcher, G. W. and Howard, J. C. (1977) Nature 266: 550–552.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • Tomoyuki Kawamoto
    • 1
  • J. Denry Sato
    • 1
  • Anh Le
    • 1
  • Don B. McClure
    • 1
  • Gordon H. Sato
    • 1
  1. 1.Cancer Center, Q-058University of CaliforniaSan Diego, La JollaUSA

Personalised recommendations