Analysis of the Mitogenic Effect of Fibrinogen
Fibrinogen is absent in serum but is a major component of plasma, where its concentration is about 3 mg/ml. Blood clotting results from the polymerisation of fibrinogen into fibrin (l). Here we present evidence that fibrinogen may play another role by stimulating the proliferation of certain human hemopoietic cells. A strong proliferative response of certain cell lines resulted from the synergism elicited by the addition of fibrinogen (3–30 nM) to transferrin in defined medium. Fibrinogen was also found to increase the plating efficiency and colony size when human bone marrow cells were cultured in semi-solid media containing serum. Purified fibrinogen fragment D possessed a stimulating activity similar to that of the intact fibrinogen molecule. This fragment cannot form fibrin thus eliminating fibrin as a source of the mitogenic effect.
KeywordsMitogenic Effect Plating Efficiency Colony Assay Human Bone Marrow Cell Normal Human Bone Marrow
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