Adipose Conversion of Murine Bone Marrow-Derived Macrophages in Serum-Free Medium

  • Inge Flesch
  • Uwe-Peter Ketelsen
  • Ernst Ferber
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


A serum-free culture medium to obtain macrophages from the murine bone marrow was established. The medium includes sodium pyruvate, sodium selenite, insuline, transferrin, either linoleic or oleic acid, bovine serum albumin and L-cell supernatant. In the case of oleic acid macrophage colonies are obtained. If linoleic acid is added, macrophages are developing which convert into fat cells. Obviously linoleic acid has adipogenic activity.


Linoleic Acid Oleic Acid Sodium Selenite Murine Bone Marrow Sodium Hydrogen Carbonate 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • Inge Flesch
    • 1
  • Uwe-Peter Ketelsen
    • 1
  • Ernst Ferber
    • 1
  1. 1.Max-Panck-Institut für ImmunbiologieFreiburgGermany

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