Adipose Conversion of Murine Bone Marrow-Derived Macrophages in Serum-Free Medium
A serum-free culture medium to obtain macrophages from the murine bone marrow was established. The medium includes sodium pyruvate, sodium selenite, insuline, transferrin, either linoleic or oleic acid, bovine serum albumin and L-cell supernatant. In the case of oleic acid macrophage colonies are obtained. If linoleic acid is added, macrophages are developing which convert into fat cells. Obviously linoleic acid has adipogenic activity.
KeywordsAlbumin Pyruvate Hydrogen Carbonate Transferrin Phosphatidylcholine
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