Advertisement

Hormonal Regulation of Growth and Function of Insulin-Producing Cells in Culture

  • J. Høiriis Nielsen
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

The endocrine pancreas in mammals is organized in numerous small clusters of cells, the islets of Langerhans, consisting of 1000–3000 cells of which the insulin-producing beta-cells amount to about 60–70%. The islets represent only 1–2% of the total pancreas, and it was not before the introduction of the collagenase digestion technique (Moskalewski, 1965) that larger quantities of isolated pancreatic islets could be obtained for biochemical studies (for a review, see Nielsen and Lernmark, 1983).

Keywords

Growth Hormone Beta Cell Pancreatic Islet Insulin Release Insulin Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Andersson A (1975) Synthesis of DNA in isolated pancreatic islets maintained in tissue culture. Endocrinology 96: 1051–1054.CrossRefGoogle Scholar
  2. Andersson A (1978) Isolated mouse pancreatic islets in culture: Effects of serum and different culture media on the insulin production of the islets. Diabetologia 14: 397–404.PubMedCrossRefGoogle Scholar
  3. Andersson A, Hellerstrom C (1972) Metabolic characteristics of isolated pancreatic islets in tissue culture. Diabetes 21 (suppl. 2): 546–554.PubMedGoogle Scholar
  4. Billaudel B, Sutter BCJ (1981) Modulation of the direct effect of corticosteroid upon glucose-induced insulin secretion of rat isolated islets of Langerhans. Diab. Metab. 7: 91–96.Google Scholar
  5. Borelli MI, Garcia ME, Durum CLG, Gagliardino JJ (1982) Glucocorticoid- induced changes in insulin secretion related to the metabolism and ultrastructure of pancreatic islets. Horm. Metab. Res. 14: 287–292.PubMedCrossRefGoogle Scholar
  6. Borg LAH (1982) Effects of steroid hormones on intracellular insulin degradation in isolated islets of Langerhans of the mouse. Diabetologia 23: 157.Google Scholar
  7. Brunstedt J (1980) Rapid isolation of functionally intact pancreatic islets from mice and rats by Percoll TMgradient centrifugation. Diab. Metab. 6: 87–89.Google Scholar
  8. Brunstedt J (1982) Expression of the insulin gene: Regulation by glucose, hydrocortisone, and growth hormone. Special FEBS Meeting on Cell Function and Differentiation, April 25–29, 1982 Athens, Greece, Abstracts p. 149.Google Scholar
  9. Brunstedt J, Nielsen JH (1981) Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture. Acta Endocr. (Cph) 96: 498–504.Google Scholar
  10. Chick WL (1973) Beta cell replication in rat pancreatic monolayer cultures. Effects of glucose, tolbutamide, glucocorticoid, growth hormone and glucagon. Diabetes 22: 687–693.PubMedGoogle Scholar
  11. Fong HKW, Chick WL, Sato G (1981) Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium. Diabetes 30: 1022–1028.PubMedCrossRefGoogle Scholar
  12. Green IC, Taylor KW (1972) Effects of pregnancy in the rat on the size and insulin secretory response of the islets of Langerhans. J. Endocrinol. 54: 317–325.PubMedCrossRefGoogle Scholar
  13. Heding L (1971) Radioimmunological determination of pancreatic and gut glucagon in plasma. Diabetologia 7: 10–19.PubMedCrossRefGoogle Scholar
  14. Hellerstrom C (1963) Effects of steroid diabetes on the pancreatic islets of guinea pigs with special reference to the A, cells. Acta Soc. Med. Upsal. 68: 1–16.Google Scholar
  15. Kern H, Logothetopoulos J (1970) Steroid diabetes in the guinea pig. Diabetes 19: 145–154.PubMedGoogle Scholar
  16. King DL, Chick WL (1976) Pancreatic beta cell replication: Effects of hexose sugars. Endocrinology 99: 1003–1009.PubMedCrossRefGoogle Scholar
  17. Logothetopoulos J (1972) Islet cell regeneration and neogenesis. In: Steiner DF, Freinkel H (eds.) Handbook of Physiology, Sect. 7, vol. 1. American Physiological Society, Washington DC, pp. 67–76.Google Scholar
  18. Moskalewski S (1965) Isolation and culture of the islets of Langer¬hans of the guinea pig. Gen. Comp. Endocr. 5: 342–353.CrossRefGoogle Scholar
  19. Nielsen JH (1981) Beta cell function in isolated human pancreatic islets in long-term tissue culture. Acta Biol. Med. Germ. 40: 55–60.PubMedGoogle Scholar
  20. Nielsen JH (1982) Effects of growth hormone, prolactin, and placental lactogen on insulin content and release and deoxyribonucleic acid synthesis in cultured pancreatic islets. Endocrinology 110: 600–606.PubMedCrossRefGoogle Scholar
  21. Nielsen JH, Brunstedt K (1982) Growth hormone as a growth factor for normal pancreatic islet cells in primary culture. In: Sirbasku DA, Sato G (eds.): Growth of cells in hormonally defined media. Cold Spring Habor Conference on -Cell Proliferation vol. 9 (in press).Google Scholar
  22. Nielsen JH, Lernmark A (1983) Purification of islets and cells from islets. In: Pretlow TG and Pretlow T (eds.) Cell Separation: Methods and selected applications. Academic Press, New York, vol. 2 (in press).Google Scholar
  23. Nielsen JH, Brunstedt J, Andersson A, Frimodt-M0ller C (1979) Preservation of beta cell function in adult human pancreatic islets for several months in vitro. Diabetologia 16: 97–100.PubMedCrossRefGoogle Scholar
  24. Nielsen JH, J0rgensen R, Jensen D, Brunstedt K (1981) Direct effect of pregnancy hormones on growth and function of pancreatic islets in tissue culture. Diabetologia 21: 308–309.CrossRefGoogle Scholar
  25. Pierluissi J, Pierluissi R, Ashcroft SJH (1982) Effects of hypophysectomy and growth hormone on cultured islets of Langerhans of the rat. Diabetologia 22: 134–137.PubMedGoogle Scholar
  26. Rabinovitch A, Blondel B, Murray T, Mintz DH (1980) Cyclic adenosine-351-monophosphate stimulates islet B cell replication in neonatal pancreatic monolayer cultures. J. Clin. Invest. 66: 1065–1071.PubMedCrossRefGoogle Scholar
  27. Rabinovitch A, Quigley C, Russell T, Patel Y, Mintz DH (1982) Insulin and multiplication stimulating activity (an insulin-like growth factor) stimulate islet β-cell replication in neonatal rat pancreatic monolayer cultures. Diabetes 31: 160–164.PubMedCrossRefGoogle Scholar
  28. Rabinovitch A, Quigley C, Rechler MM (1983) Growth hormone stimulates β-cell replication in neonatal rat pancreatic monolayer cultures. Diabetes (in press).Google Scholar
  29. Swenne I, Hellerstrom C (1982) Pancreatic β-cell regeneration in rats of various ages. Diabetologia 23: 203.CrossRefGoogle Scholar
  30. Swenne I, Bone AJ, Howell SL, Hellerstrom C (1980) Effects of glucose and amino acids on the biosynthesis of DNA and insulin in fetal rat islets maintained in tissue culture. Diabetes 29: 686–692.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • J. Høiriis Nielsen
    • 1
  1. 1.Hagedorn Research LaboratoryGentofteDenmark

Personalised recommendations