Hormonal Regulation of Growth and Function of Insulin-Producing Cells in Culture
The endocrine pancreas in mammals is organized in numerous small clusters of cells, the islets of Langerhans, consisting of 1000–3000 cells of which the insulin-producing beta-cells amount to about 60–70%. The islets represent only 1–2% of the total pancreas, and it was not before the introduction of the collagenase digestion technique (Moskalewski, 1965) that larger quantities of isolated pancreatic islets could be obtained for biochemical studies (for a review, see Nielsen and Lernmark, 1983).
KeywordsGrowth Hormone Beta Cell Pancreatic Islet Insulin Release Insulin Content
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- Billaudel B, Sutter BCJ (1981) Modulation of the direct effect of corticosteroid upon glucose-induced insulin secretion of rat isolated islets of Langerhans. Diab. Metab. 7: 91–96.Google Scholar
- Borg LAH (1982) Effects of steroid hormones on intracellular insulin degradation in isolated islets of Langerhans of the mouse. Diabetologia 23: 157.Google Scholar
- Brunstedt J (1980) Rapid isolation of functionally intact pancreatic islets from mice and rats by Percoll TMgradient centrifugation. Diab. Metab. 6: 87–89.Google Scholar
- Brunstedt J (1982) Expression of the insulin gene: Regulation by glucose, hydrocortisone, and growth hormone. Special FEBS Meeting on Cell Function and Differentiation, April 25–29, 1982 Athens, Greece, Abstracts p. 149.Google Scholar
- Brunstedt J, Nielsen JH (1981) Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture. Acta Endocr. (Cph) 96: 498–504.Google Scholar
- Hellerstrom C (1963) Effects of steroid diabetes on the pancreatic islets of guinea pigs with special reference to the A, cells. Acta Soc. Med. Upsal. 68: 1–16.Google Scholar
- Logothetopoulos J (1972) Islet cell regeneration and neogenesis. In: Steiner DF, Freinkel H (eds.) Handbook of Physiology, Sect. 7, vol. 1. American Physiological Society, Washington DC, pp. 67–76.Google Scholar
- Nielsen JH, Brunstedt K (1982) Growth hormone as a growth factor for normal pancreatic islet cells in primary culture. In: Sirbasku DA, Sato G (eds.): Growth of cells in hormonally defined media. Cold Spring Habor Conference on -Cell Proliferation vol. 9 (in press).Google Scholar
- Nielsen JH, Lernmark A (1983) Purification of islets and cells from islets. In: Pretlow TG and Pretlow T (eds.) Cell Separation: Methods and selected applications. Academic Press, New York, vol. 2 (in press).Google Scholar
- Rabinovitch A, Quigley C, Rechler MM (1983) Growth hormone stimulates β-cell replication in neonatal rat pancreatic monolayer cultures. Diabetes (in press).Google Scholar