Advertisement

Primary Rat Astroglial Culture in Serum-Free Supplemented Media and Effect of a Bovine Brain Factor

  • M. Weibel
  • B. Pettmann
  • G. Daune
  • G. Labourdette
  • M. Sensenbrenner
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

A serum-free supplemented medium has been developed that supports growth of astroglial cells in primary culture from newborn rat brain hemispheres. This medium consists of a basal medium (Waymouth MD 705/1) supplemented with insulin (I), transferrin (T) and fatty-acid-free bovine serum albumin (BSA). In basal medium without serum only a few cells survived after 15 days. In the presence of I and T the number of cells increased. The same results was observed with the addition of a purified fraction from bovine brain extract (astroglial growth factor: AGF). Addition of AGF to the IT medium stimulated the proliferation of the cells and elicited an increase of the level of S-100 protein and of the activity of glutamine synthetase. The complete defined medium (with I, T and BSA) supported the growth of astroglial cells. After 14 days nearly all cells express glial fibrillary acidic (GFA) protein. These cells could be maintained in the defined medium over a period of 4 weeks.

Keywords

Linoleic Acid Glial Fibrillary Acidic Protein Glutamine Synthetase Bovine Brain Astroglial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Pettmann, B., Sensenbrenner, M. and Labourdette, G. (1980) FEBS Letters 118, 195.PubMedCrossRefGoogle Scholar
  2. 2.
    Brunner, G., Lang, K., Wolfe, R.A., McClure, D.B. and Sato, G.H. (1981) Develop. Brain Res., 2, 563.CrossRefGoogle Scholar
  3. 3.
    Morrison, R.S. and De Vellis, J. (1981) Proc. Natl. Acad. Sci. U.S.A., 78, 7205.PubMedCrossRefGoogle Scholar
  4. 4.
    Fischer, G., Lentz, A. and Schachner, M. (1982) Neurosci. Letters, 29, 297.CrossRefGoogle Scholar
  5. 5.
    Hinegardner, R.T. (1971) Anal. Biochem. 39, 197.PubMedCrossRefGoogle Scholar
  6. 6.
    Labourdette, G. and Marks, A. (1975) Eur. J. Biochem., 8, 73.CrossRefGoogle Scholar
  7. 7.
    Miller, R.E., Hackenberg, R. and Gershman, H. (1978) Proc. Natl. Acad. Sci. U.S.A., 75, 1418.PubMedCrossRefGoogle Scholar
  8. 8.
    Pettmann, B., Weibel, M., Daune, G., Sensenbrenner, M. and Labourdette, G., J. Neurosci. Res. (in press).Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • M. Weibel
    • 1
  • B. Pettmann
    • 1
  • G. Daune
    • 1
  • G. Labourdette
    • 1
  • M. Sensenbrenner
    • 1
  1. 1.Centre de Neurochimie du CNRS and INSERM U44Strasbourg CedexFrance

Personalised recommendations