Selection and Cultivation of Astrocytes at Different Developmental Stages

  • Günther Fischer
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Important aims in neurobiology are to understand the processes which determine the differentiation of neural cells in brain tissue; of primary interest is that of the main neural cell types neurons, astrocytes and oligodendrocytes. One may assume that signals via direct cell-cell contact and interactions of cells with the extracellular matrix are important as well as signals via diffusible factors like hormones, growth or differentiation factors. Because such complex signals can often not be readily studied in detail in intact tissue, cell cultures have been utilized to follow differentiation of cells in less complex systems. The best experimental control of the cellular environment (contact environment and composition of diffusible factors) is thought to be possible in monolayer cultures. Therefore, an idealized experiment would be to start with a homogenous population of precursor cells of one of the main neural cell types which will become fully differentiated cells after a series of defined manipulations concerning cell contact environment, growth and differentiation factors, hormonal and nutritional requirements.


Epidermal Growth Factor Hyaluronic Acid Glial Fibrillary Acidic Protein Horse Serum Mouse Cerebellar 
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  1. 1.
    Poduslo, S.E. and Norton, W.T. (1972). J. Neurochem. 19: 727–736PubMedCrossRefGoogle Scholar
  2. 2.
    Snyder, D.S., Raine, C.S., Faroog, M., and Norton, W.T. (1980) J. Neurochem. 35: 1614–1620CrossRefGoogle Scholar
  3. 3.
    Campbell, G. LeM., Schachner, M. and Shannon, S.O. (1977) Brain Res. 127: 69–86PubMedCrossRefGoogle Scholar
  4. 4.
    Meier, D.H., Lagenaur, C., and Schachner, M. (1982) J. Neurosci. Res. 7: 119–134PubMedCrossRefGoogle Scholar
  5. 5.
    Varon, S. and Raiborn, Jr. C.W. (1969) Brain Res. 12: 180–199PubMedCrossRefGoogle Scholar
  6. 6.
    Barde, Y.A., Edgar, D., and Thoenen, H. (1980) Proc. Nat. Acad. Sci. USA 77: 1199–1203.PubMedCrossRefGoogle Scholar
  7. 7.
    McCarthy, K.D. and DeVellis, J. (1980) J. Cell Biol. 85: 890–902PubMedCrossRefGoogle Scholar
  8. 8.
    Brunner, G., Lang, K., Wolfe, R.A., McClure, D.B., and Sato, G.H. (1981) Develop. Brain Res. 2: 563–575CrossRefGoogle Scholar
  9. 9.
    Fischer, G. (1982) Neurosci. Lett. 28: 325–29PubMedCrossRefGoogle Scholar
  10. 10.
    Fischer, G., Leutz, A., and Schachner, M. (1982) Neurosci. Lett. 29: 297–302PubMedCrossRefGoogle Scholar
  11. 11.
    Betz, H. and Müller, K. (1982) Exp. Cell Res. 138: 297–302PubMedCrossRefGoogle Scholar
  12. 12.
    Wakade, A.R., Edgar, D., and Thoenen, H. (1982) Exp. Cell Res. 140: 71–78PubMedCrossRefGoogle Scholar
  13. 13.
    Morrison, R.S. and DeVellis, J. (1981) Proc. Nat. Acad. Sci. USA 78: 7205–7209PubMedCrossRefGoogle Scholar
  14. 14.
    Schnitzer, J., Franke, W.W., and Schachner, M. (1981) J. Cell Biol. 90: 435–447PubMedCrossRefGoogle Scholar
  15. 15.
    Dahl, D., Rueger, D.C., Bignami, A., Weber, K., and Osborn, M. (1981) Eur. J. Cell Biol. 24: 191–196PubMedGoogle Scholar
  16. 16.
    Bignami, A., Dahl, D., and Rueger, D.C. (1980) Advanc. Neurobiol. 1: 285–310Google Scholar
  17. 17.
    Schachner, M. (1982) J. Neurochem. 39: 1–8PubMedCrossRefGoogle Scholar
  18. 18.
    Schachner, M., Schoonmaker, G., and Hynes, R.O. (1978) Brain Res. 158: 149–158PubMedCrossRefGoogle Scholar
  19. 19.
    Raju, T., Bignami, A., and Dahl, D. (1981) Dev. Biol. 85: 344–357PubMedCrossRefGoogle Scholar
  20. 20.
    Savage, R.C., Jr. and Cohen, S. (1972) J. Biol. Chem. 247: 7609–7611PubMedGoogle Scholar
  21. 21.
    Raff, M.C., Mirsky, R., Fields, K.L., Lisak, R.P., Dorfman, S.H., Silberberg, D.H., Gregson, N.A., Liebowitz, S., and Kennedy, M. (1978) Nature 274: 813–816PubMedGoogle Scholar
  22. 22.
    Rathjen, F. and Schachner, M. (1983) EMBO J., in pressGoogle Scholar
  23. 23.
    Leutz, A. and Schachner, M. (1981) Cell Tissue Res. 220: 393–404PubMedCrossRefGoogle Scholar
  24. 24.
    Fischer, G. and Schachner, M. (1982) Exp. Cell Res. 139: 285–296PubMedCrossRefGoogle Scholar
  25. 25.
    Fischer, G., in preparationGoogle Scholar
  26. 26.
    Lim, R. (1980) Curr. Top. Dev. Biol. 16: 305–322PubMedCrossRefGoogle Scholar
  27. 27.
    Weibel, W., Pettmann, B., Daune, G., Labourdette, G., and Sensenbrenner, M., this volumeGoogle Scholar
  28. 28.
    Nexd, E., Hollenberg, M.D., Figueroa, A., and Pratt, R.M. (1980) Proc. Nat. Acad. Sci. USA 77: 2782–2785CrossRefGoogle Scholar
  29. 29.
    Fischer, G., in preparationGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • Günther Fischer
    • 1
  1. 1.Institut für NeurobiologieUniversität HeidelbergHeidelbergGermany

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