Selection and Cultivation of Astrocytes at Different Developmental Stages
Important aims in neurobiology are to understand the processes which determine the differentiation of neural cells in brain tissue; of primary interest is that of the main neural cell types neurons, astrocytes and oligodendrocytes. One may assume that signals via direct cell-cell contact and interactions of cells with the extracellular matrix are important as well as signals via diffusible factors like hormones, growth or differentiation factors. Because such complex signals can often not be readily studied in detail in intact tissue, cell cultures have been utilized to follow differentiation of cells in less complex systems. The best experimental control of the cellular environment (contact environment and composition of diffusible factors) is thought to be possible in monolayer cultures. Therefore, an idealized experiment would be to start with a homogenous population of precursor cells of one of the main neural cell types which will become fully differentiated cells after a series of defined manipulations concerning cell contact environment, growth and differentiation factors, hormonal and nutritional requirements.
KeywordsEpidermal Growth Factor Hyaluronic Acid Glial Fibrillary Acidic Protein Horse Serum Mouse Cerebellar
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