Cells of the Peripheral Nervous System; Requirements for Expression of Function in Tissue Culture

  • R. P. Bunge
  • D. J. Carey
  • D. Higgins
  • C. Eldridge
  • D. Roufa
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Utilizing mixtures of cell types, complex media, and a collagen substratum, it is possible to obtain a remarkable degree of functional expression in nerve tissue in culture, including myelination, synaptogenesis and histotypic organization (for review see Bunge, 1975; Crain, 1976 and Fischbach and Nelson, 1977). In order to gain an understanding of the contribution of individual cell types to specific functions, and to delineate the precise nutritional and environmental requirements for these functions, it is necessary to define media and substratum components and to study pure cell populations. Furthermore, because certain cell functions are only observed when cell types are cocultured, it is necessary to study cell types when separated and after recombination. In this chapter we briefly describe recent experience with this approach to the study of autonomic and sensory neurons, and of Schwann cells, in the peripheral nervous system. The chapter is primarily a review of work from our laboratory; space does not allow a general review.


Schwann Cell Dorsal Root Ganglion Neuron Basal Lamina Sympathetic Neuron Embryo Extract 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • R. P. Bunge
    • 1
  • D. J. Carey
    • 1
  • D. Higgins
    • 1
  • C. Eldridge
    • 1
  • D. Roufa
    • 1
  1. 1.Department of Anatomy and NeurobiologyWashington University School of MedicineSt. LouisUSA

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