Cells of the Peripheral Nervous System; Requirements for Expression of Function in Tissue Culture
Utilizing mixtures of cell types, complex media, and a collagen substratum, it is possible to obtain a remarkable degree of functional expression in nerve tissue in culture, including myelination, synaptogenesis and histotypic organization (for review see Bunge, 1975; Crain, 1976 and Fischbach and Nelson, 1977). In order to gain an understanding of the contribution of individual cell types to specific functions, and to delineate the precise nutritional and environmental requirements for these functions, it is necessary to define media and substratum components and to study pure cell populations. Furthermore, because certain cell functions are only observed when cell types are cocultured, it is necessary to study cell types when separated and after recombination. In this chapter we briefly describe recent experience with this approach to the study of autonomic and sensory neurons, and of Schwann cells, in the peripheral nervous system. The chapter is primarily a review of work from our laboratory; space does not allow a general review.
KeywordsSchwann Cell Dorsal Root Ganglion Neuron Basal Lamina Sympathetic Neuron Embryo Extract
Unable to display preview. Download preview PDF.
- Bottenstein, J.E. 1982 Growth requirements of neural cells in vitro. In: Advances in Cellular Neurobiology (in press).Google Scholar
- Bunge, R.P. 1975 Changing uses of nerve tissue culture 1950–1975. In: The Nervous System, D.B. Tower, ed., Raven Press, New York, pp. 31–42.Google Scholar
- Bunge, R.P., Bunge, M.B., Carey, D.J., Cornbrooks, C.J., Higgins, D.H., Johnson, M.I., Iacovitti, L., Kleinschmidt, D.C., Moya, F. and Wood, P. 1982 Functional expression in primary nerve tissue cultures maintained in defined medium. Cold Spring Harbor Conferences on Cell Proliferation. 9: 1017–1031.Google Scholar
- Cornbrooks, C., Carey, D., Timpl, R., McDonald, J. and Bunge, R. 1982a Immunohistochemical visualization of fibronectin and laminin in adult rat peripheral nerve and peripheral nerve cells in culture. Soc. Neurosci. Abstr. 8: 240.Google Scholar
- Cornbrooks, C.J., Mithen, F., Cochran, J.M. and Bunge, R.P. 1982b Factors affecting Schwann cell basal lamina formationin cultures of dorsal root ganglia from mice with muscular dystrophy. Dev. Brain Res. (in press).Google Scholar
- Crain, S. 1976. Neurophysiology Studies in Tissue Culture. Raven Press, New York.Google Scholar
- Fischbach, G.D. and Nelson, P.G. 1977 Cell culture in neurobiology. In: Handbook of Neurophysiology, E. Kandel, ed., pp. 719–774.Google Scholar
- Landis, S.C. and Keefe, D. 1980 Development of cholinergic sympathetic innervation of eccrine sweatglands in rat footpad. Soc. Neurosci. Abstr. 6: 379.Google Scholar
- Roufa, D.G., Johnson, M.I. and Bunge, M.B. 1982a Influence of ganglion age, non- neuronal cells and substratum in neurite outgrowth in culture. (submitted for publication).Google Scholar
- Roufa, D., Cornbrooks, C., Johnson, M. and Bunge, M. 1982b Preparation of homogenous populations of immature Schwann cells from early embryonic sympathetic ganglia. Soc. Neurosci. Abstr. 8: 240.Google Scholar