Advertisement

Use of Eye Derived Growth Factor from Retina (EDGF) in a Defined Medium for the Culture of Bovine Epithelial Lens Cells

  • J. Plouët
  • M. Olivié
  • J. Courty
  • Y. Courtois
  • D. Barritault
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Summary

We have previously described that retina and other ocular tissues (iris, choroid, vitreous body) contain a growth factor named Eye Derived Growth Factor or EDGF different from Epidermal Growth Factor (EGF) and Fibroblast Growth Factor (FGF). Since proliferation of bovine epithelial lens cells (BEL cells) is stimulated by addition of EDGF or FGF but not of EGF when cultured in presence of optimal fetal calf serum concentration (6%) in MEM medium, we looked at the effect of these growth factors on proliferation of subcultured BEL cells in a serum free medium. MEM 199 - HAM F12 (1 : 1) supplemented with insulin and transferrin (5 μg × ml-1), of EDGF or 10 ng × ml-1 of EGF provided an increase of 70%. No synergistic effect on proliferation was observed with any combination of these growth factors. Hydrocortisone (10-8 M) in combination with EDGF provided an increase in proliferation up to 80% of the serum supplemented control, however the cells elongate and looked so different from normal cells that this combination was not used. In the presence of EDGF the following hormones were inefficient: T3 (10-12 to 10-9M), PGE1, (1 to 100 ng × ml-1), LHRF (1to - 100 ng × ml-1), testosterone or progesterone (0,1 to 10 ng × ml-1).

Keywords

Epidermal Growth Factor Fibroblast Growth Factor Ocular Tissue Vitreous Body Epithelial Lens Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Reddan J.R., Cell Biology of the Eye. 1982, 299–375.Google Scholar
  2. 2.
    Courtois Y., Simonneau L., Tassin J., Laurent M. and Malaise E., Differentiation. 1978, 10, 23–30.PubMedCrossRefGoogle Scholar
  3. 3.
    Courtois Y., Arruti C., Barritault D., Tassin J., Olivie M. and Hughes R.C., Differentiation, 1981, 18, 11–27.PubMedCrossRefGoogle Scholar
  4. 4.
    Arruti C., Courtois Y., Exp. Cell Res, 1978, 117, 283–291.PubMedCrossRefGoogle Scholar
  5. 5.
    Barritault D., Arruti C. and Courtois Y., Differentiation, 1981, 18, 29–42.PubMedCrossRefGoogle Scholar
  6. 6.
    Barritault D., Plouet J., Courty J. and Courtois Y. Journal of Neurosc. Res., 1982, in press.Google Scholar
  7. 7.
    Plouet J., Barritault D., Courtois Y. and Ladda R., FEBS Letters, 1982, 144, 85–88.PubMedCrossRefGoogle Scholar
  8. 8.
    Gospodarowicz D., Mescher A., Brown K. and Birdwell R., Exp. Eye Res, 1977, 25, 631–649.PubMedCrossRefGoogle Scholar
  9. 9.
    Van Venrooij W.J., Groeneveld A.A., Bloemendal H. and Benedetti E.L., Exp. Eye Res, 1974, 18, 527–536.PubMedCrossRefGoogle Scholar
  10. 10.
    Reddan J.R., De Hart D.J. and Sackman J.E., J. Cell Biol., 1981, 91., 148.CrossRefGoogle Scholar
  11. 11.
    Gospodarowicz D. and Massoglia S., Exp. Eye Res, 1982, 35, 259–270.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • J. Plouët
    • 1
  • M. Olivié
    • 1
  • J. Courty
    • 1
  • Y. Courtois
    • 1
  • D. Barritault
    • 1
    • 2
  1. 1.INSERM U.118, CNRS ERA 842ParisFrance
  2. 2.University of Paris XIIFrance

Personalised recommendations