Synergistic Effect of Retinoic Acid on DNA Synthesis of Prostaglandin F Stimulated Swiss 3T3 Cells

  • M. Eschenbruch
  • A. M. Otto
  • L. Jimenez de Asua
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


In confluent resting Swiss mouse 3T3 cells, prostaglandin F (PGF) stimulates the initiation of DNA synthesis and cell division. This effect is enhanced by insulin and reflected by an increase in the rate into S phase (1). Recently it has been demonstrated that PGE1 and PGE2 (at concentrations that do not stimulate DNA synthesis) have a synergistic effect on PGF alone, or with insulin (2). In this communication we report that retinoic acid, as well as analogues of retinoic acid, enhanced the effect of PGF and insulin. This synergy was not further increased by PGE1 or PGE2. Indomethacin, an inhibitor of prostaglandin synthesis, did not affect the synergy of retinoic acid with PGF, indicating that retinoic acid did not extert its effect by increasing prostaglandin synthesis.


Retinoic Acid Retinyl Acetate Keto Derivative Ethyl Amide Special Fellow 
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  1. 1.
    Jimenez de Asua, L., O’Farrell, M.K., Clingan, D. and Rudland, P., Proc.Natl.Acad.Sci.USA, 74 (1977), 3845.PubMedCrossRefGoogle Scholar
  2. 2.
    Otto, A.M., Nilsen-Hamilton, M., Boss, B.D., Ulrlich, M.-O. and Jimenez de Asua, L., Proc.Natl.Acad.Sci.USA, 79 (1982), 4992.PubMedCrossRefGoogle Scholar
  3. 3.
    Todaro, G.J. and Green, H., J.Cell Biol. 17 (1967), 299.CrossRefGoogle Scholar
  4. 4.
    Bollag, W. and Matter, A., Ann.N.Y.Acad.Sci. 359 (1981), 9.PubMedCrossRefGoogle Scholar
  5. 5.
    Shier, W.T. and Durkin, J.P., J.Cell.Physiol. 112 (1982), 171.PubMedCrossRefGoogle Scholar
  6. 6.
    De Luca, L.M. and Shapiro, S.S., eds. “Modulation of cellular Interactions by Vitamin A and Derivatives (Retinoids)”. Ann.N.Y.Acad. Sci., Vol. 359, 1981.Google Scholar
  7. 7.
    Lacroix, A., Anderson, G.D.L. and Lippman, M.E., Exp.Cell Res. 130 (1980), 339.PubMedCrossRefGoogle Scholar
  8. 8.
    Jetten, A.M., Jetten, M.E.R., Shapiro, S.S. and Poon, J.P., Exp. Cell Res. 119 (1979), 289.PubMedCrossRefGoogle Scholar
  9. 9.
    Dion, L.D., Blalock, J.E. and Grifford, G.E., J.Natl.Cancer Inst. 58 (1977), 795.PubMedGoogle Scholar
  10. 10.
    Lotan, R., Neumann, G. and Lotan, D., Ann.N.Y.Acad.Sci. 359 (1981), 150.PubMedCrossRefGoogle Scholar
  11. 11.
    Patt, L.M., Itaya, K. and Hakomori, S.-I. Nature 273 (1978), 379.PubMedCrossRefGoogle Scholar
  12. 12.
    Todaro, G.J., DeLarco, J.E. and Sporn, M.B., Nature 276 119781, 272.Google Scholar
  13. 13.
    Kensler, T.W., Mueller, G.C., Cancer Nature 38 (1978), 771.Google Scholar
  14. 14.
    Dicker, P, and Rozenqurt, E., Biochem. Biophys. Res. com. 91 (1979), 1203.PubMedCrossRefGoogle Scholar
  15. 15.
    Jetten, A.M., Nature 284 (1980), 626.PubMedCrossRefGoogle Scholar
  16. 16.
    Deluca, L.M. Vitamines and Hormones 35 (1977), 1.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • M. Eschenbruch
    • 1
  • A. M. Otto
    • 1
  • L. Jimenez de Asua
    • 1
  1. 1.Friedrich Miescher-InstitutBaselSwitzerland

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