Monoclonal Antibodies to Interferons
The introduction of a technique for the production of monoclonal antibodies by hybrid myelomas (hybridomas) (Kohler and Milstein 1975) is leading to the widespread replacement of conventional antisera by monoclonal antibodies in many areas where antibodies are used. Conventional antisera generally contain a heterogeneous population of antibodies of different affinities and recognizing a variety of different antigenic determinants. The mixture of antibodies can vary not only from animal to animal, but even in successive bleeds from a single animal and this has led to difficulties in the standardization of antisera and of substances measured by antibodies. Monoclonal antibodies are chemically pure, recognize a single determinant with a unique affinity, and can be produced over a long period of time without any change of properties.
KeywordsSugar Carbohydrate Titration Glycol Electrophoresis
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