Kinetics and Thermodynamics of Reactions Catalyzed by Penicillin Acylase — Type Enzymes

  • Jan Konecny
Conference paper

Summary

The thermodynamics and mechanism of reactions catalyzed by penicillin acylase-type enzymes, as seen at present, are outlined. Most if not all that is known about these proteins is consistent with the view that they are hydrolases of both esters and amides of α-aminoacids like phenyglycine and, in some cases, also of structurally related compounds like phenylacetic acid. They apparently react, like chymotrypsin, via an acyl-enzyme intermediate which then transfers the acyl group to water, amines or alcohols as receptors.

Keywords

Hydrolysis Amide Glycine Phenyl Penicillin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Rolinson GN, Batchelor FR, Butterworth D, Cameron-Wood J, Cole M, Eustace GC, Hart MV, Richards M, Chain EB (1960) Nature 187: 236CrossRefGoogle Scholar
  2. 2.
    Kaufmann W, Bauer K (1960) Naturwissenschaften 40: 474CrossRefGoogle Scholar
  3. 3.
    Claridge CA, Gourevitch A, Lein J (1960) Nature 187: 237CrossRefGoogle Scholar
  4. 4.
    Matsumoto K (1980) Hakko to Kogyo 38: 216Google Scholar
  5. 5.
    Okachi R (1979) Nippon Nogei Kagaku Kaishi 53: R169CrossRefGoogle Scholar
  6. 6.
    Vandamme EJ (1977) Adv Appl Microbiol 21: 89CrossRefGoogle Scholar
  7. 7.
    Vandamme EJ, Voets JP (1974) Adv Appl Microbiol 17: 311CrossRefGoogle Scholar
  8. 8.
    Vandamme EJ (1981) J Chem Tech Biotechnol 31: 637CrossRefGoogle Scholar
  9. 9.
    Kato K (1980) Agric Biol Chem 44: 1083CrossRefGoogle Scholar
  10. 10.
    Svedas VK, Margolin AL, Berezin IV (1980) Enz Microbiol Technol 2: 138CrossRefGoogle Scholar
  11. 11.
    Konecny J (1981) Biotechnol Letters 3: 107Google Scholar
  12. 12.
    Svedas VK, Margolin AL, Borisov EL, Berezin IV (1980) Enz Microbiol Technol 2: 313CrossRefGoogle Scholar
  13. 13.
    Konecny J, Sieber M, Schneider A (1981) Biotechnol Letters 3: 507CrossRefGoogle Scholar
  14. 14.
    Konecny J, Sieber M, Schneider A (in press) Biotechnol BioengGoogle Scholar
  15. 15.
    McDougal B, Dunnill P, Lilly MD (1982) Enz Microb Technol 4: 114CrossRefGoogle Scholar
  16. 16.
    Semenov AM, Martinek K, Shvyadas VYu, Margolin AL, Berezin IV (1981) Dokl Akad Nauk SSSR 258: 1124Google Scholar
  17. 17.
    Takahashi T, Kato K, Yamazaki Y, Isono M (1977) J Antibiotics 30 Suppl, 230Google Scholar
  18. 18.
    Takasawa S, Okachi R, Kawamoto I, Yamamoto M, Nara T (1972) Agr Biol Chem 36: 1701CrossRefGoogle Scholar
  19. 19.
    Okachi R, Kawamoto I, Yamamoto M, Takasawa S, Nara T (1973) Agr Biol Chem 37: 335CrossRefGoogle Scholar
  20. 20.
    Bondareva NS, Levitov MM, Rabinovitsch MS (1969) Biokhimia (Engl transl) 34: 378Google Scholar
  21. 21.
    Chiang C, Bennett RE (1967) J Bacteriol 93: 302Google Scholar
  22. 22.
    Balasingham K, Warburton D, Dunnill P, Lilly MD (1972) Biochim Biophys Acta 276: 250Google Scholar
  23. 23.
    Kutzbach C, Rauenbusch E (1974) Z Physiol Chem 354: 45CrossRefGoogle Scholar
  24. 24.
    Margolin AL, Svedas VK, Berezin IV (1980) Biochim Biophys Acta 616: 283Google Scholar
  25. 25.
    Okachi R, Nara T (1973) Agr Biol Chem 37: 2797CrossRefGoogle Scholar
  26. 26.
    Shimizu M, Okachi R, Kimura K, Nara T (1975) Agr Biol Chem 39: 1655CrossRefGoogle Scholar
  27. 27.
    Kato K, Kawahara K, Takahashi T, Kakinuma A (1980) Agric Biol Chem 44: 1075CrossRefGoogle Scholar
  28. 28.
    Takahashi T, Yamazaki Y, Kato K (1974) Biochem J 137: 497Google Scholar
  29. 29.
    Marconi W, Bartoli F, Cecere F, Galli G, Morisi F (1975) Agric Biol Chem 39: 277CrossRefGoogle Scholar
  30. 30.
    Antonov VK (1981) in “Proteinases and their Inhibitors” (Turk V, Vitale L eds), p 141, Pergamon Press, OxfordGoogle Scholar
  31. 31.
    Fujii T, Matsumoto K, Watanabe T (1976) Process Biochem 11 [8]: 1Google Scholar
  32. 32.
    Okachi R, Kato F, Miyamura Y, Nara T (1973) Agr Biol Chem 37: 1953CrossRefGoogle Scholar
  33. 33.
    Shimizu M, Masuike T, Fujita H, Kimura K, Okachi R, Nara T (1975) Agr Biol Chem 39: 1225CrossRefGoogle Scholar
  34. 34.
    Kato K, Kawahara K, Takahashi T, Igarazi S (1980) Agr Biol Chem 44: 821CrossRefGoogle Scholar
  35. 35.
    Kasche V, Galunsky B (1982) Biochem Biophys Res Comm 104: 1215CrossRefGoogle Scholar

Copyright information

© Springer-Verlag, Berlin, Heidelberg 1983

Authors and Affiliations

  • Jan Konecny

There are no affiliations available

Personalised recommendations