Different Pathways of Chloroform Metabolism

  • E. Testai
  • L. Vittozzi
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 7)


Chloroform metabolism can occur in liver, under physiological pO2 conditions, with and without participation of O2. Although, under anaerobic conditions the protein covalent binding of CHCl3 metabolites is lowered, cytochrome P-450 decrease is more substantial. The loss of cytochrome P-450 cannot be accounted for either by heme destruction or lipid peroxidation under anaerobic conditions. It is not prevented by GSH. Different spectral changes occur between 400 and 500 nm, depending on the oxygen concentration; transition takes place at 1–6% pO2.

Key words

Chloroform Metabolism Cytochrome P-450 Rat liver Microsomes Oxygen concentration 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ahmed AE, Kubic VL, Anders MW (1977) Metabolism of haloform to carbon monoxide. I. In vitro studies. Drug Metab Dispos 5: 198–204Google Scholar
  2. Ilett KF, Reid WD, Sipes IG, Krishna G (1973) Chloroform toxicity in mice: correlation of renal and hepatic necrosis with covalent binding of metabolites to tissue macromolecules. Exp Mol Pathol 19:215–229PubMedCrossRefGoogle Scholar
  3. Mansuy D, Beaune P, Cresteil T, Lange M, Leroux JP (1977) Evidence for phosgene formation during liver microsomal oxidation of chloroform. Biochem Biophys Res Commun 79:513–517PubMedCrossRefGoogle Scholar
  4. Pohl RL, Bhooshan B, Whittaker NF, Krishna G (1977) Phosgene: a metabolite of chloroform. Biochem Biophys Res Commun 79: 684–691PubMedCrossRefGoogle Scholar
  5. Pohl RL, Martin JL, George W (1980) Mechanism of metabolic activation of chloroform by rat liver microsomes. Biochem Pharmacol 29: 3271–3276PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • E. Testai
    • 1
  • L. Vittozzi
    • 1
  1. 1.Department of Comparative Toxicology and EcotoxicologyIstituto Superiore di SanitàRomeItaly

Personalised recommendations