Skip to main content
SpringerLink
Log in

Cellular Aspects of Amino-Acid Transport

  • Conference paper
Book cover Intestinal Transport

Part of the book series: Proceedings in Life Sciences ((LIFE SCIENCES))

Abstract

The object of the present review is to describe the characteristics of amino acid transport studied at the cellular level. Such studies have been carried out using enterocytes isolated from villi; they have also involved transport studies carried out on enterocytes cultured from the intestine of germ-free animals. More recently, studies have also been carried out using whole tissue, enterocytes involved in amino acid transport being identified and analysed subsequently using quantitative autoradiography.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bass R, Hedegaard HB, Dillehay L, Moffet J, Englesberg E (1981) The A, ASC and L systems for the transport of amino acids in Chinese hamster ovary cells. J Biol Chem 265:10259–10266

    Google Scholar 

  • Cremaschi D, James PS, Meyer G, Peacock MA, Smith MW (1982) Membrane potentials of differentiating enterocytes. Biochim Biophys Acta 688:271–274

    Article  PubMed  CAS  Google Scholar 

  • Curran PF, Schultz SG, Chez RA, Fuisz RE (1967) Kinetic relations of the Na-amino acid interaction at the mucosal border of intestine. J Gen Physiol 50:1261–1286

    Article  PubMed  CAS  Google Scholar 

  • Danisi G, Tai Y-H, Curran PF (1976) Mucosal and serosal fluxes of alanine in rabbit ileum. Biochim Biophys Acta 455:200–213

    Article  PubMed  CAS  Google Scholar 

  • Garvey TQ, Hyman PE, Isselbacher KJ (1976) 7-Glutamyl transpeptidase of rat intestine: Localization and possible role in amino acid transport. Gastroenterology 71:778–785

    PubMed  CAS  Google Scholar 

  • Gazzola GC, Dall’Asta V, Guidotti GG (1980) The transport of neutral amino acids in cultured human fibroblasts. J Biol Chem 255:929–936

    PubMed  CAS  Google Scholar 

  • Handlogten ME, Garcia-Cafiero R, Lancaster KT, Christensen HN (1981) Surprising differences in substrate selectivity and other properties of systems A and ASC between rat hepatocytes and the hepatoma cell line HTC. J Biol Chem 256:7905–7909

    PubMed  CAS  Google Scholar 

  • Inui KI, Quaroni A, Tillotson LG, Isselbacher KJ (1980) Amino acid and hexose transport by cultured crypt cells from rat small intestine. Am J Physiol 239:C190-C196

    PubMed  CAS  Google Scholar 

  • James PS, Smith MW (1980) Amino acid-induced changes in intestinal short-circuit current. J Physiol (Lond) 303:79P

    CAS  Google Scholar 

  • James PS, Smith MW (1981) A preparation of intestinal villi suitable for recording membrane potentials in enterocytes taken at different stages of differentiation. J Physiol (Lond) 319: 6–7P

    Google Scholar 

  • Kedinger M, Simon PM, Grenier JF, Haffen K (1981) Role of epithelial-mesenchymal interactions in the ontogenesis of intestinal brush-border enzymes. Devel Biol 86:339–347

    Article  CAS  Google Scholar 

  • Kilberg MS, Lancaster KT, Christensen HN (1981) Neutral amino acid transport in isolated rat intestinal segments. Fed Proc 39: abstr 570

    Google Scholar 

  • Kimmich GA (1975) Preparation and characterization of isolated intestinal epithelial cells and their use in studying intestinal transport. In: Korn E (ed) Methods in membrane biology, vol IV. Plenum, New York, pp 51–115

    Google Scholar 

  • King IS, Sepúlveda FV, Smith MW (1981) Cellular distribution of neutral and basic amino acid transport systems in rabbit ileal mucosa. J Physiol (Lond) 319:355–368

    CAS  Google Scholar 

  • Kinter WB, Wilson TH (1965) Autoradiography study of sugar and amino acid absorption by everted sacs of hamster intestine. J Cell Biol 25:19–39

    Article  PubMed  CAS  Google Scholar 

  • Lipkin EM (1981) Proliferation and differentiation of gastrointestinal cells in normal and disease states. In: Physiology of the gastrointestinal tract, vol I. Raven Press, New York, pp 145–168

    Google Scholar 

  • Menge H, Hopert R, Alexopoulos T, Riecken EO (1982a) Three-dimensional structure and cell kinetics at different sites of rat intestinal remnants during the early adaptive response to resection. Res Exp Med 181:77–94

    Article  CAS  Google Scholar 

  • Menge H, Sepúlveda FV, Smith MW (1982b) Cellular adaptation of amino acid transport following intestinal resection in the rat. J Physiol (Lond) 334:213–233

    Google Scholar 

  • Munck BG (1981) Intestinal absorption of amino acids. In: Physiology of the gastrointestinal tract, vol II. Raven Press, New York, pp 1097–1122

    Google Scholar 

  • Munck BG, Schultz SG (1969) Lysine transport across isolated rabbit ileum. J Gen Physiol 53: 157–182

    Article  PubMed  CAS  Google Scholar 

  • Paterson JYF, Smith MW (1982) Testing the hypothesis that substrate availability determines the cellular distribution of amino acid uptake by rabbit ileal mucosa. J Physiol (Lond) 327: 96–97P

    Google Scholar 

  • Paterson JYF, Sepúlveda FV, Smith MW (1979) Two-carrier influx of neutral amino acids into rabbit ileal mucosa. J Physiol (Lond) 292:339–350

    CAS  Google Scholar 

  • Paterson JYF, Sepúlveda FV, Smith MW (1980a) Stoichiometry versus coupling ratio in the co-transport of Na and different neutral amino acids. Biochim Biophys Acta 603:288–297

    Article  PubMed  CAS  Google Scholar 

  • Paterson JYF, Sepúlveda FV, Smith MW (1980b) A sodium-independent low affinity transport system for neutral amino acids in rabbit ileal mucosa. J Physiol (Lond) 298:333–346

    CAS  Google Scholar 

  • Paterson JYF, Sepúlveda FV, Smith MW (1981) Distinguishing transport systems having overlapping specificities for neutral and basic amino acids in the rabbit ileum. J Physiol (Lond) 319:345–354

    CAS  Google Scholar 

  • Paterson JYF, Sepúlveda FV, Smith MW (1982a) Distribution of transported amino acid within rabbit ileal mucosa. J Physiol (Lond) 331:523–535

    CAS  Google Scholar 

  • Paterson JYF, Sepúlveda FV, Smith MW (1982b) Amino acid efflux from rabbit ileal enterocytes. J Physiol (Lond) 331:537–546

    CAS  Google Scholar 

  • Reiser S, Christiansen PA (1971) The properties of the preferential uptake of L-leucine by isolated intestinal epithelial cells. Biochim Biophys Acta 225:123–139

    Article  PubMed  CAS  Google Scholar 

  • Sepúlveda FV, Pearson JD (1982) Characterization of neutral amino acid uptake by cultured epithelial cells from pig kidney. J Cell Physiol 112:182–188

    Article  PubMed  Google Scholar 

  • Sepúlveda FV, Smith MW (1978) Discrimination between different entry mechanisms for neutral amino acids in rabbit ileal mucosa. J Physiol (Lond) 282:73–90

    Google Scholar 

  • Sepúlveda FV, Burton KA, Brown PD (1982) Relation between sodium-coupled amino acid and sugar transport and sodium/potassium pump activity in isolated intestinal epithelial cells. J Cell Physiol 111:303–308

    Article  PubMed  Google Scholar 

  • Shapiro MP, Kimmich GA (1981) Na+:L-valine coupling stoichiometry in isolated intestinal cells. Fed Proc 39:abstr569

    Google Scholar 

  • Smith MW (1981) Autoradiographic analysis of alanine uptake by newborn pig intestine. Experientia 37:868–869

    Article  PubMed  CAS  Google Scholar 

  • Smith MW, Jarvis LG, King IS (1980) Cell proliferation in follicle-associated epithelium of mouse Peyer’s patch. Am J Anat 159:157–166

    Article  PubMed  CAS  Google Scholar 

  • Smith MW, Syme G (1982) Functional differentiation of enterocytes in the follicle-associated epithelium of rat Peyer’s patch. J Cell Sci 55:147–156

    PubMed  CAS  Google Scholar 

  • Søgnen E (1967) A method for the preparation of suspensions of intestinal mucosal cells by means of calcium chelators. Acta Vet Scand 8:76–82

    Google Scholar 

  • Stevens BR, Ross HJ, Wright EM (1982) Multiple transport pathways for neutral amino acids in rabbit jejunal brush border vesicles. J Membr Biol 66:213–225

    Article  PubMed  CAS  Google Scholar 

  • Syme G (1982) The effect of protein-deficient isoenergetic diets on the growth of rat jejuna mucosa. Br. J Nutr 48:25–36

    Article  PubMed  CAS  Google Scholar 

  • Syme G, Smith MW (1982) Intestinal adaptation to protein deficiency. Cell Biol Int Rep 6:573–578

    Article  PubMed  CAS  Google Scholar 

  • Weiser MM (1973) Intestinal epithelial cell surface membrane glycoprotein synthesis. An indicator of cellular differentiation. J Biol Chem 248:2536–2541

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. A.R.C. Institute of Animal Physiology, CB2 4AT, Babraham, Cambridge, Great Britain

    J. Y. F. Paterson

Authors
  1. M. W. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. V. Sepúlveda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Y. F. Paterson
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Laboratory of General and Comparative Biochemistry, University of Liège, 17, Place Delcour, 4020, Liège, Belgium

    M. Gilles-Baillien (Scientific Editor) (Scientific Editor)

  2. Laboratory of Animal Physiology, University of Liège, 22, Quai Van Beneden, 4020, Liège, Belgium

    R. Gilles (Coordinating Editor) (Coordinating Editor)

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Smith, M.W., Sepúlveda, F.V., Paterson, J.Y.F. (1983). Cellular Aspects of Amino-Acid Transport. In: Gilles-Baillien, M., Gilles, R. (eds) Intestinal Transport. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-69109-6_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-69109-6_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-69111-9

  • Online ISBN: 978-3-642-69109-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation