A Novel Micromethod for the Limulus Amebocyte Lysate (LAL) Assay for Endotoxin, Based on Hydrostatic Pressure

  • I. Neeman
  • M. Mittelman
  • S. L. Gaffin
Conference paper
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 6)

Abstract

A micromethod for performing the Limulus amebocyte lysate (LAL) assay for the presence of endotoxin is described which requires only about five percent of the standard amount of LAL reagent. The method is based upon measuring an increased amount of hydrostatic pressure required to cause a gelled LAL sample to flow from a capillary tube. The method is simple, rapid and correlates well with the results of the standard LAL assay procedure.

Key words

Micromethod LAL assay Endotoxin Hydrostatic Pressure 

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References

  1. Dubczak J, Cotter R, Dastoli F (1979) Quantitative detection of endotoxin by nephelometry. In: Cohen E (ed) Biomedical applications of the horseshoe crab. Liss, New York, pp 403–414Google Scholar
  2. Frauch P (1974) The slide test as a micromethod of a modified Limulus endotoxin test. J Pharm Sci 63: 808–809PubMedCrossRefGoogle Scholar
  3. Gaffin SL (1979) Endotoxin determination in viscous opaque solutions of iron dextran by Limulus amebocyte lysate. In: Cohen E (ed) Biomedical applications of the horseshoe crab. Liss, New York, pp 221–227Google Scholar
  4. Gaffin SL, Obedeanu N, Merzbach D (1979) The Limulus amebocyte lysate test for endotoxin in human plasma. Thromb Haemost 42: 808–809PubMedGoogle Scholar
  5. Harris N, Feinstein (1977) A new Limulus assay for the detection of endotoxin. J Trauma 17: 714–718PubMedCrossRefGoogle Scholar
  6. Hollander V, Harding W (1976) A sensitive spectrophotometric method for measurement of plasma endotoxin. Biochem Med 15: 28–33PubMedCrossRefGoogle Scholar
  7. Jary MJ, Margitic S (1979) Comparison of homogenizing shaking and blending on the recovery of microorganisms and endotoxins from fresh and frozen ground beef as assayed by plate counts and Limulus amebocyte lysate test. Appl Environ Microbiol 38: 879Google Scholar
  8. Jorgensen JA, Lee JC, Pahren HR (1976) Rapid detection of bacterial endotoxins in drinking water. Appl Environ Microbiol 32: 347PubMedGoogle Scholar
  9. Kimura H (1976) Measurement of endotoxin. I. Fundamental studies on radioimmunoassay of endotoxin. Acta Med Okayama 30: 245–255PubMedGoogle Scholar
  10. Levin J, Bang F (1968) Cottable protein in Limulus: Its localization and kinetics of its coagulation by endotoxin. Thromb Haemost 19: 186–197Google Scholar
  11. Mascoli C, Weary M (1979) Applications and advantages of the LAL pyrogen test for parenteral injectable products. In: Cohen E (ed) Biomedical applications of the horseshoe crab. Liss, New York, pp 387–402Google Scholar
  12. Niwa M, Hiramatsu T, Waguri O (1974) The gelation reaction between horsehoe crab amebocytes and endotoxins studied by the quantitative clot method. Jpn J Med Sci Biol 27: 108–111PubMedGoogle Scholar
  13. Randolph W (1977) Licensing of Limulus amebocyte lysate. Fed Reg 42: 57749–57750Google Scholar
  14. Reinhold R, Fine J (1971) A technique for quantitative measurement of endotoxin in human plasma. Proc Soc Exp Biol Med 137: 334–339PubMedGoogle Scholar
  15. Tai C (1974) Clinical and experimental endotexima related to the intestinal ischemia. Detection of endoxin by means of radioimmunoassay. Jpn J Med Sci Biol 27: 111–114PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • I. Neeman
    • 1
  • M. Mittelman
    • 1
  • S. L. Gaffin
    • 2
  1. 1.Department of Food Engineering and BiotechnologyTechnion-Israel Institute of TechnologyHaifaIsrael
  2. 2.Department of Physiology and BiophysicsTechnion-Israel Institute of TechnologyHaifaIsrael

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