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Biochemical Aspects of Fluoroacetate Poisoning in Isolated Rat Kidney Tubules: Reversibility of Inhibition of Gluconeogenesis by α-Ketoglutarate

  • P. Wiedemann
  • L. Szinicz
  • N. Weger
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 6)

Abstract

The effects of monofluoroacetate (FAC) on suspensions of isolated rat kidney tubules were investigated. FAC inhibited gluconeogenesis from lactate, pyruvate, fructose, dihydroxyacetone, α-ketoglutarate, and succinate. The gluconeogenesis from pyruvate, ketoglutarate, and lactate was less sensitive to FAC than that from other substrates. FAC also caused a decrease in oxygen consumption, hydroxybutyrate to acetoacetate ratio, α-ketoglutarate, ATP, and total adenine nucleotide content; the citrate content was increased.

Addition of α-ketoglutarate, 5 mmol/l, caused a reversal of gluconeogenesis inhibition, an increase in ATP content, and a delay in citrate accumulation in isolated rat kidney tubules incubated with FAC.

Key words

Fluoroacetate Isolated rat kidney tubules Gluconeogenesis α-Ketoglutarate 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • P. Wiedemann
    • 1
  • L. Szinicz
    • 1
  • N. Weger
    • 1
  1. 1.Pharmakologisches Institut der Medizinischen FakultätLudwig-Maximilian-Universität MünchenMünchen 2Federal Republic of Germany

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