Biochemical Aspects of Fluoroacetate Poisoning in Isolated Rat Kidney Tubules: Reversibility of Inhibition of Gluconeogenesis by α-Ketoglutarate
The effects of monofluoroacetate (FAC) on suspensions of isolated rat kidney tubules were investigated. FAC inhibited gluconeogenesis from lactate, pyruvate, fructose, dihydroxyacetone, α-ketoglutarate, and succinate. The gluconeogenesis from pyruvate, ketoglutarate, and lactate was less sensitive to FAC than that from other substrates. FAC also caused a decrease in oxygen consumption, hydroxybutyrate to acetoacetate ratio, α-ketoglutarate, ATP, and total adenine nucleotide content; the citrate content was increased.
Addition of α-ketoglutarate, 5 mmol/l, caused a reversal of gluconeogenesis inhibition, an increase in ATP content, and a delay in citrate accumulation in isolated rat kidney tubules incubated with FAC.
Key wordsFluoroacetate Isolated rat kidney tubules Gluconeogenesis α-Ketoglutarate
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