Summary
The cytochemical localisation of five hydrolytic enzymes has been studied in the brain capillaries of laboratory animals. Acid phosphatase is present in primary lysosomes of endothelial cells; alkaline phosphatase activity is seen mainly on the plasma membrane of the luminal side but also in the basal lamina. The latter is also active concerning 5’nucleotidase. Butyrylcholinesterase is an enzyme synthesized by most brain capillary endothelial cells, as can be seen by intensive staining of endoplasmic reticulum cisternae. In contrast acetylcholinesterase activity at the capillaries presumably is of neuronal origin. Local neurons appear to secrete this enzyme, which then reaches the endothelial basal lamina via the extracellular spaces. From these cytochemical observations it is concluded that pinocytotic traffic in brain endothelial cells is predominantly from the brain tissue side to the luminal side.
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References
Dempsey EW, Wislocki GB (1955) An electron microscopic study of the blood-brain barrier of the rat, employing silver nitrate as a vital stain. J biophys biochem Cytol 1: 245–256
Brightman MW, Reese TS (1969) Junctions between intimately apposed cell membranes in the vertebrate brain. J Cell Biol 40: 648–677
Reese TS, Karnovska MJ (1967) Fine structural localization of a blood-brain barrier to exogenous peroxidase. J Cell Biol 34: 207–217
Brightman MW, Klatzko I, Olsson Y, Reese TS (1970) The blood-brain barrier to proteins under normal and pathological conditions. J neurol sci 10: 215–239
Kreutzberg GW, Hager H (1966) Electron microscopical demonstration of acid phosphatase activity in the central nervous system. Histochemie 6: 254–259
Mayahara H, Hirano H, Saito T, Ogawa K (1967) The new lead citrate method for the ultracytochemical demonstration of activity of non-specific alkaline phosphatase (orthophos-phoric monoesterphoshydrolase). Histochemie 11: 88–96
Wachstein M, Meisel E (1957) Histochemistry of hepatic phosphatases at a physiologic pH with special reference to the demonstration of bile canaliculi. Amer J clin Path 27: 13–23
Kreutzberg GW, Barron KD, Schubert P (1978) Cytochemical localization of 5′nucleotidase in glial plasma membranes. Brain Res 158: 247–257
Lewis PR, Shute CC (1969) An electron microscopic study of cholinesterase in the rat adrenal medulla. J Microsc (Oxford) 89: 181–193
Kaiya H, Moriuchi I, Mikami T, Weikert M, Kreutzberg GW (1976) Increase of alkaline phosphatase in the blood vessel walls as a parameter for axon reaction and its blocking by actinomycin D. Adv Neurol Sci (Tokio) 20: 131–139
Rowan RA, Maxwell DS (1981) An ultrastructural study of vascular proliferation and vascular alkaline phosphatase activity in the developing cerebral cortex of the rat. Amer J Anat 160: 257–265
Kreutzberg GW, Tóth L, Weikert M, Schubert P (1974) Changes in perineuronal capillaries accompanying chromatolysis of motoneurons. In: Cervos-Navarro J (ed) Pathology of Cerebral Microcirculation. Walter de Gruyter, Berlin, New York, pp 282–288
Schrader J, Wahl M, Kuschinsky W, Kreutzberg GW (1980) Increase of adenosine content in cerebral cortex of the cat during bicuculline-induced seizure. Pflügers Arch 387: 245–251
Berne RM, Rubio R, Curnish RR (1974) Release of adenosine from ischemic brain. Circ Res 35: 262–271
Rubio R, Berne RM, Winn HR (1978) Production, metabolism and possible functions of adenosine in brain tissue in situ. In: Cerebral Vascular Smooth Muscle and its Control (Ciba Foundation Symposium 56 (new series)) Elsevier Excerpta Medica, Amsterdam Oxford New York, pp 355–373
Flumerfelt BA, Lewis PR, Gwyn DG (1973) Cholinesterase activity of capillaries in the rat brain. A light and electron microscopic study. Histochemical J 5: 67–77
Koelle GB (1954) The histochemical localization of cholinesterase in the central nervous system of the rat. J comp Neurol 100: 211–235
Karcsú S, Tóth L (1982) Die Veränderungen der Butyryl-cholinesterase-Aktivität der fenestrierten Capillaren in der Area postrema während der postnatalen Entwicklung. Acta histochem 71: 83–94
Joó F, Scillik B (1966) Topographical correlation between the hematoencephalic barrier and the cholinesterase activity of brain capillaries. Exp Brain Res 1: 147–151
Chubb IW, Hodgson AJ, White GH (1980) Acetylcholinesterase hydrolyzes Substance P. Neuroscience 5: 2965–2972
Kreutzberg GW, Kaiya H, Tóth L (1979) Distribution and origin of acetylcholinesterase activity in the capillaries of the brain. Histochemistry 61: 111–122
Butcher LL, Talbot K (1978) Acetylcholinesterase in rat nigro-neostriatal neurons: experimental verification and evidence for cholinergic-dopaminergic interactions in the substantia nigra and caudate-putamen complex. In: Butcher LL (ed) Cholinergic-Monoaminergic Interactions in the Brain, Academic Press, New York San Francisco London, pp 25–95
Kreutzberg GW, Tóth L (1974) Dendritic secretion: a way for the neuron to communicate with the vasculature. Naturwissenschaften 61: 37
Kreutzberg GW, Tóth L, Kaiya H (1975) Acetylcholinesterase as a marker for dendritic transport and dendritic secretion. Adv Neurol 12: 269–281
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Kreutzberg, G.W., Tóth, L. (1983). Enzyme Cytochemistry of the Cerebral Microvessel Wall. In: Hossmann, KA., Klatzo, I. (eds) Cerebrovascular Transport Mechanisms. Acta Neuropathologica Supplementum, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68970-3_3
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DOI: https://doi.org/10.1007/978-3-642-68970-3_3
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