The Use of Staphylococcal Protein A in Diagnostic Virology

  • Douglas D. Richman
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 104)

Abstract

Staphylococcal protein A (SPA), a protein component of the cell wall of Staphylococcus aureus, binds the Fc portion of many immunoglobulins of most mammalian species. Verwey first described SPA in 1940 as a protein antigen present in type A staphylococci (coagulase-positive, a-toxin-positive, mannitol-fermenting, and pathogenic; i.e., S. aureus), but not in type B staphylococci (those lacking these characteristics). The systematic study of antigen A did not begin in earnest until two decades later (Jensen et al. 1961; Lofkvist and Sjoquist 1963; Grov et al. 1964). Grov and associates proposed the designation “protein A” to avoid confusion with the antigenic polysaccharide A (Grov et al. 1964).

Keywords

Vortex Hepatitis Leukemia Fractionation Polypeptide 

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References

  1. Atanasiu P, Perrin P (1979) Micromethod for rabies antibody detection by immunoenzymatic assay with staphylococcus protein A. Ann Microbiol (Paris) 130A: 257–268Google Scholar
  2. Bachi T, Dorval G, Wigzell H, Binz H (1977) Staphylococcal protein A in immunoferritin techniques. Scand J Immunol 6: 241–246PubMedCrossRefGoogle Scholar
  3. Beck OE (1981) Distribution of virus antibody activity among human IgG subclasses. Clin Exp Immunol 43: 626–632PubMedGoogle Scholar
  4. Biberfeld P, Ghetie V, Sjoquist J (1975) Demonstration and assaying of IgG antibodies in tissues and on cells by labeled staphylococcal protein A. J Immunol Methods 6: 249–259CrossRefGoogle Scholar
  5. Bind JL, Chiron JP, Denis F (1978) Protein A research in 1200 strains of staphylococcus (using a rapid detection technic by passive hemagglutination with glutaraldehyde-treated erythrocytes). CR Soc Biol (Paris) 172: 212–215Google Scholar
  6. Bjork I, Petersson BA, Sjoquist J (1972) Some physicochemical properties of protein A from Staphylococcus aureus. Eur J Biochem 29: 579–584PubMedCrossRefGoogle Scholar
  7. Bommeli W, Kihm U, Zindel F, Fey H (1980) Enzyme-linked immunoassay and fluorescent antibody techniques in the diagnosis of viral diseases using staphylococcal protein-A instead of anti-gamma-globulins. Vet Immunol Immunopathol 1: 179–193PubMedCrossRefGoogle Scholar
  8. Bradley DW, Fields HA, McCaustland KA, Maynard JE, Decker RH, Whittington R, Overby LR (1979) Serodiagnosis of viral hepatitis A by a modified competitive binding radioimmunoassay for immunoglobulin M anti-hepatitis A virus. J Clin Microbiol 9: 120–127PubMedGoogle Scholar
  9. Braun R, Doerr HW, Geisen HP, Hornig C, Huschka U, Munk K (1981) Comparison of different methods for the detection of rubella-specific IgM antibodies. J Med Virol 8: 207–214PubMedGoogle Scholar
  10. Brown JP, Klitzman JM, Hellstrom KE (1977) A microassay for antibody binding to tumor cell surface antigens using 12SI-labelled protein A from Staphylococcus aureus. J Immunol Methods 15: 57–66PubMedCrossRefGoogle Scholar
  11. Buchanan D, Kamarck M, Ruddle NH (1981) Development of a protein A enzyme immunoassay for use in screening hybridomas. J Immunol Methods 42: 179–185PubMedCrossRefGoogle Scholar
  12. Burnette WN (1981) “Western Blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112:195–203PubMedCrossRefGoogle Scholar
  13. Bystricka M, Rajcani J, Foldes O, Sadlon J (1980) Detection of herpesvirus antigens by solid-phase radioimmunoassay with 1251-antiglobulin and 125I-protein A. Acta Virol (Praha) 24: 265–272Google Scholar
  14. Callis AH, Ritzi EM (1981a) Estimation of mouse mammary tumor virus gp52 abundance by protein A assay: a comparison of viral antigen expression on C3H and GR mammary tumor cells. Intervirology 15: 111–120PubMedCrossRefGoogle Scholar
  15. Callis AH, Ritzi EM (1981b) Protein A assay for mouse mammary tumor virus gp52 determinants on murine and human mammary tumor cells. Virology 111: 656–661PubMedCrossRefGoogle Scholar
  16. Celio MR, Lutz H, Binz H, Fey H (1979) Protein A in immunoperoxidase techniques. J Histochem Cytochem 27: 691–698PubMedCrossRefGoogle Scholar
  17. Chalon MP, Milne RW, Vaerman JP (1979) Interactions between mouse immunoglobulins and staphylococcal protein A. Scand J Immunol 9: 359–364PubMedCrossRefGoogle Scholar
  18. Cleveland PH, Richman DD, Oxman MN, Wickham MG, Binder PS, Worthen DM (1979) Immobilization of viral antigens on filter paper for a [125I]staphylococcal protein A immunoassay: a rapid and sensitive technique for detection of herpes simplex virus antigens and antiviral antibodies. J Immunol Methods 29: 369–386PubMedCrossRefGoogle Scholar
  19. Cleveland PH, Richman DD, Oxman MN, Worthen DM (1982) Rapid serologic technique for typing herpes simplex viruses. J Clin Microbiol 15: 402–407PubMedGoogle Scholar
  20. Cleveland PH, Richman DD, Redfield DC, Disharoon DR, Binder PS, Oxman MN (to be published) An enzyme immunofiltration technique for the rapid diagnosis of herpes simplex virus eye infections. J Clin MicrobiolGoogle Scholar
  21. Coe JE, Coe PR, Ross MJ (1981) Staphylococcal protein A purification of rodent Ig, and Ig with particular emphasis on Syrian hamsters. Mol Immunol 18: 1007–1012PubMedCrossRefGoogle Scholar
  22. Colburn KK, Wong LG, Ashman RF, Wistar R Jr, Weisbart RH (1980) Effect of immunoglobulin G on the responsiveness of human neutrophils to soluble staphylococcal protein A-induced neutrophil migration inhibition factor from T-lymphocytes. Infect Immun 30:674– 677PubMedGoogle Scholar
  23. Colombatti A, Hilgers J (1979) A radioimmunoassay for virus antibody using binding of 125I- labelled protein A. J Gen Virol 43: 395–401PubMedCrossRefGoogle Scholar
  24. Costa J, Yee C, Nakamura Y, Rabson A (1978) Characteristics of the Fc receptor induced by herpes simplex virus. Intervirology 10: 32–39PubMedCrossRefGoogle Scholar
  25. Crawford LV, Lane DP (1977) An immune complex assay for SV40 T antigen. Biochem Biophys Res Commun 74: 323–329PubMedCrossRefGoogle Scholar
  26. Crovari P, Gasparini R, Bono A, Lusian F, Tassi GC (1979) Use of staphylococcal protein A for the detection of rubella virus IgM antibodies. Boll 1st Sieroter Milan 58: 371–381Google Scholar
  27. Crowther JR, Abu Elzein EME (1980) Detection of antibodies against foot-and-mouth disease virus using purified staphylococcus A protein conjugated with alkaline phosphatase. J Immunol Methods 34: 261–267PubMedCrossRefGoogle Scholar
  28. Deisenhofer J (1981) Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9-and 2.8-A resolution. Biochemistry 20: 2361–2370PubMedCrossRefGoogle Scholar
  29. Deisenhofer J, Jones TA, Huber R, Sjodahl J, Sjoquist J (1978) Crystallization, crystal structure analysis and atomic model of the complex formed by a human Fc fragment and fragment B or protein A from Staphylococcus aureus. Hoppe Seyler Z Physiol Chem 359: 975–985PubMedCrossRefGoogle Scholar
  30. Dorval G, Welsh KI, Wigzell H (1975) A radioimmunoassay of cellular surface antigens on living cells using iodinated soluble protein A from Staphylococcus aureus. J Immunol Methods 7: 237–250PubMedCrossRefGoogle Scholar
  31. Dossett JH, Kronvall G, Williams RC Jr, Quie PG (1969) Antiphagocytic effects of staphylococcal protein A. J Immunol 103: 1405–1410Google Scholar
  32. Dubois-Dalcq M, McFarland H, McFarlin D (1977) Protein A-peroxidase: a valuable tool for the localization of antigens. J Histochem Cytochem 25: 1201–1206PubMedCrossRefGoogle Scholar
  33. Duhamel RC, Meezan E, Brendel K (1980) The pH-dependent binding of goat IgGl and IgG2 to protein A-sepharose. Mol Immunol 17: 29–36PubMedCrossRefGoogle Scholar
  34. Endresen C (1978) Protein A reactivity of whole rabbit IgG and of fragments of rabbit IgG. Acta Pathol Microbiol Scand [C] 86: 211–214Google Scholar
  35. Engvall E (1978) Preparation of enzyme-labelled staphylococcal protein A and its use for detection of antibodies. Scand J Immunol [Suppl 7] 8: 25–31CrossRefGoogle Scholar
  36. Enzmann PJ (1978) Solid-phase indirect radioimmunoassays for rapid diagnosis of Sindbis virus antigen. J Gen Virol 41: 641–644PubMedCrossRefGoogle Scholar
  37. Field PR, Shanker S, Murphy AM (1980) The use of protein A-sepharose affinity chromatography for separation and detection of specific IgM antibody in acquired rubella infection: a comparison with absorption by staphylococci containing protein A and density gradient ultracentrifugation. J Immunol Methods 32: 59–70PubMedCrossRefGoogle Scholar
  38. Figenschau KJ, Ulstrup JC (1974) Staphylococcal radioimmunoassay for hepatitis B antigen and antibody. Acta Pathol Microbiol Scand [B] 82: 422–428Google Scholar
  39. Filice GA, Yeager AS, Remington JS (1980) Diagnostic significance of immunoglobulin M antibodies to Toxoplasma gondii detected after separation of immunoglobulin M from immunoglobulin G antibodies. J Clin Microbiol 12: 336–342PubMedGoogle Scholar
  40. Forsgren A (1968) Protein A from Staphylococcus aureus. VI Reaction with subunits from guinea pig and globulin. J Immunol 100: 927–930PubMedGoogle Scholar
  41. Forsgren A (1970) Significance of protein A production by staphylococci. Infect Immun 2:672– 673PubMedGoogle Scholar
  42. Forsgren A (1972) Pathogenicity of Staphylococcus aureus mutants in general and local infections. Acta Pathol Microbiol Scand [B] 80: 564–570Google Scholar
  43. Forsgren A, Sjoquist J (1966) Protein A from S. aureus. I. Pseudo-Immune reaction with human 7-globulin. J Immunol 97: 822–827PubMedGoogle Scholar
  44. Forsum U, Hjelm E, Jonsell G (1976) Antibody-coated bacteria in the urine of children with urinary tract infections. Acta paediatr Scand 65: 639–642PubMedCrossRefGoogle Scholar
  45. Furukawa T, Hornberger E, Sakuma S, Plotkin SA (1975) Demonstration of immunoglobulin G receptors induced by human cytomegalovirus. J Clin Microbiol 2: 332–336PubMedGoogle Scholar
  46. Geuze HJ, Slot JW, van der Ley PA, Scheffer RCT (1981) Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections. J Cell Biol 89:653– 665PubMedCrossRefGoogle Scholar
  47. Ghetie V, Nilsson K, Sjoquist J (1974a) Identification of cell surface immunoglobulin markers by protein A-containing fluorescent staphylococci. Scand J Immunol 3: 397–403PubMedCrossRefGoogle Scholar
  48. Ghetie V, Nilsson K, Sjoquist J (1974b) Detection and quantitation of immunoglobulin G on the surface of human lymphoid cells by rosette formation with protein A-coated sheep red blood cells. Eur J Immunol 4: 500–509PubMedCrossRefGoogle Scholar
  49. Goding JW (1976) Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods 13: 215–226PubMedCrossRefGoogle Scholar
  50. Goding JW (1978) Use of staphylococcal protein A as an immunological reagent. J Immunol Methods 20: 241–253PubMedCrossRefGoogle Scholar
  51. Goudswaard J, van der Donk JA, Noordzij A, van Dam RH, Vaerman JP (1978) Protein A reactivity of various mammalian immunoglobulins. Scand J Immunol 8: 21–28PubMedCrossRefGoogle Scholar
  52. Gough KH, Shukla DD (1980) Further studies on the use of protein A in immune electron microscopy for detecting virus particles. J Gen Virol 51: 415–419PubMedCrossRefGoogle Scholar
  53. Gross GN, Rehm SR, Toews GB, Hart DA, Pierce AK (1978) Lung clearance of Staphylococcus aureus strains with differing protein A content: protein A effect on in vivo clearance. Infect Immun 21: 7–9PubMedGoogle Scholar
  54. Grov A, Myklestad B, Oeding P (1964) Immunochemical studies on antigen preparations from Staphylococcus aureus. Acta Pathol Microbiol Scand [C] 61: 588–596Google Scholar
  55. Hayes EC, Wright LL, Zweerink HJ (1978) Staphylococcal protein-A sepharose columns and the characterization of measles virus—specific polypeptides in persistently infected cells. Anal Biochem 91: 276–282PubMedCrossRefGoogle Scholar
  56. Hebert JP, Caillet R, Hacquard B, Fortier B (1981) Utilisation de la proteine A de Staphylococcus aureus pour le depistage des rotavirus dans les celles. Pathol Biol (Paris) 29: 101–104Google Scholar
  57. Hjelm H, Hjelm K, Sjoquist J (1972) Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Lett 28: 73–76PubMedCrossRefGoogle Scholar
  58. Holmes HC, Tuffrey M, Steuden J, Hilgers J, Barnes RD (1980) The use of 12SI-labelled protein A for the detection of humoral immunity to gross murine leukaemia virus. J Immunol Methods 36: 235–241PubMedCrossRefGoogle Scholar
  59. Hooghe-Peters EL, Rentier B, Dubois-Dalcq M (1979) Electron-microscopic study of measles virus infection: unusual antibody-triggered redistribution of antigens on giant cells. J Virol 29: 666–676PubMedGoogle Scholar
  60. Hsieh S, Goldstein E, Lippert W, Margulies L (1978) Effect of protein A on the antistaphylococcal defense mechanisms of the murine lung. J Infect Dis 138: 754–759PubMedCrossRefGoogle Scholar
  61. Huang AS, Okorie T (1978) Rapid diagnosis using surface analysis by bacterial adherence. Lancet 2: 1146PubMedCrossRefGoogle Scholar
  62. Inganas M, Johanssons GO, Sjoquist J (1981) Further characterization of the alternative protein-A interaction of immunoglobulins: demonstration of an Fc-binding fragment of protein A expressing the alternative reactivity. Scand J Immunol 14: 379–388PubMedCrossRefGoogle Scholar
  63. Inman RD, McDougal JS, Redecha PB, Lockshin MD, Stevens CE, Christian CL (1981) Isolation and characterization of circulating immune complexes in patients with hepatitis B systemic vasculitis. Clin Immunol Immunopathol 21: 364–374PubMedCrossRefGoogle Scholar
  64. Inouye S, Kono R, Taksuchi Y (1978) Oligomeric immunoglobulin A antibody response to rubella virus infection. J Clin Microbiol 8: 1–6PubMedGoogle Scholar
  65. Jährling PB, Hesse RA, Metzger JF (1978) Radioimmunoassay for quantitation of antibodies to alphaviruses with staphylococcal protein A. J Clin Microbiol 8: 54–60PubMedGoogle Scholar
  66. Jankowski MA, Gut W, Switalski L, Imbs D, Kantoch M (1979) IgM fluorescence antibodies in sera of pregnant women exposed to rubella. Arch Virol 60: 123–130PubMedCrossRefGoogle Scholar
  67. Jensen K, Neter E, Gorzynski EA, Anzai H (1961) Studies on toxic products of Staphylococcus. Acta Pathol Microbiol Scand [C] 53: 191–200CrossRefGoogle Scholar
  68. Johansson SGO, Inganas M (1978) Interaction of polyclonal human IgE with protein-A from Staphylococcus aureus. Immunol Rev 41: 248–260PubMedCrossRefGoogle Scholar
  69. Jonsson S, Kronvall G (1974) The use of protein A containing Staphylococcus aureus as a solid-phase anti-IgG reagent in radioimmunoassays as exemplified in the quantitation of a-feto-protein in normal human adult serum. Eur J Immunol 4: 29–33PubMedCrossRefGoogle Scholar
  70. Kangro HO, Pattison JR, Heath RB (1978) The detection of rubella-specific IgM antibodies by radioimmunoassay. Br J Exp Pathol 59: 577–583PubMedGoogle Scholar
  71. Kessler SW (1975) Rapid isolation of antigens from cells with a staphylococcal protein A-anti-body adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol 115: 1617–1624PubMedGoogle Scholar
  72. Kessler SW (1976) Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent. J Immunol 117: 1482–1490PubMedGoogle Scholar
  73. Kessler SW (1981) Use of protein A-bearing staphylococci for the immunoprecipitation and isolation of antigens from cells. Methods Enzymol 73: 442–458PubMedCrossRefGoogle Scholar
  74. Kronvall G, Frommel D (1970) Definition of staphylococcal protein A reactivity for human immunoglobulin G fragments. Immunochemistry 7: 124–127PubMedCrossRefGoogle Scholar
  75. Kronvall G, Williams RC Jr (1969) Differences in anti-protein A activity among IgG subgroups. J Immunol 103: 828–833PubMedGoogle Scholar
  76. Kronvall G, Seal US, Finstad J, Williams RC Jr (1970a) Phylogenetic insight into evolution of mammalian Fc fragment of 7G globulin using staphylococcal protein A. J Immunol 104: 140–147PubMedGoogle Scholar
  77. Kronvall G, Quie PG, Williams RC Jr (1970b) Quantitation of staphylococcal protein A; determination of equilibrium constant and number of protein A residues on bacteria. J Immunol 104: 273–278PubMedGoogle Scholar
  78. Kronvall G, Grey HM, Williams RC Jr (1970c) Protein A reactivity with mouse immunoglobulins. J Immunol 105: 1116–1123PubMedGoogle Scholar
  79. Kronvall G, Dossett JH, Quie PG, Williams RC Jr (1971) Occurrence of protein A in staphylococcal strains; quantitative aspects and correlation to antigenic and bacteriophage types. Infect Immun 3: 10–15PubMedGoogle Scholar
  80. Lachica VF, Genigeorgis CA, Hoeprich PD (1979) Occurrence of protein A in Staphylococcus aureus and closely related Staphylococcus species. J Clin Microbiol 10: 752–753PubMedGoogle Scholar
  81. Lancet D, Isenman D, Sjodahl J, Sjoquist J, Pecht I (1978) Interaction between staphylococcal protein A and immunoglobulin domains. Biochem Biophys Res Commun 85: 608–614PubMedCrossRefGoogle Scholar
  82. Langone JJ (1978) [125I] protein A: a tracer for general use in immunoassay. J Immunol Methods 24:269–285PubMedCrossRefGoogle Scholar
  83. Langone JJ, Boyle MDP, Borsos T (1978) Studies on the interaction between protein A and immunoglobulin G. I. Effect of protein A on the functional activity of IgG. J Immunol 121: 327–332PubMedGoogle Scholar
  84. Leinikki PO, Shekarchi I, Dorsett P, Sever JL (1978) Determination of virus-specific IgM anti-bodies by using ELISA: elimination of false-positive results with protein A-Sepharose absorption and subsequent IgM antibody assay. J Lab Clin Med 92: 849–857PubMedGoogle Scholar
  85. Lindmark R, Movitz J, Sjoquist J (1977) Extracellular protein A from a methicillin-resistant strain of Staphylococcus aureus. Eur J Biochem 74: 623–628PubMedCrossRefGoogle Scholar
  86. Lofgren B, Nordenfelt E, Jonsson S, Kronvall G (1980) New bacterial absorption method for determination of hepatitis A IgM and IgA antibodies. J Med Virol 6: 37–44PubMedCrossRefGoogle Scholar
  87. Lofkvist T, Sjoquist J (1963) Purification of staphylococcal antigens. Int Arch Allergy 23: 289–305CrossRefGoogle Scholar
  88. MacSween JM, Eastwood SL (1978) Recovery of immunologically active antigen from staphylococcal protein A-antibody adsorbent. J Immunol Methods 23: 259–267CrossRefGoogle Scholar
  89. MacSween JM, Eastwood SL (1981) Recovery of antigen from staphylococcal protein A-antibody adsorbents. Methods Enzymol 73: 459–471PubMedCrossRefGoogle Scholar
  90. Madore HP, Baumgarten A (1979) Enzyme-linked protein A: an enzyme-linked immunosorbent assay reagent for detection of human immunoglobulin G and virus-specific antibody. J Clin Microbiol 10: 529–532PubMedGoogle Scholar
  91. Medgyesi GA, Fust G, Gergely J, Bazin H (1978) Classes and subclasses of rat immunoglobulins: interaction with the complement system and with staphylococcal protein A. Immunochemistry 15: 125–129PubMedCrossRefGoogle Scholar
  92. Movitz J (1976) Formation of extracellular protein A by Staphylococcus aureus. Eur J Biochem 68: 291–299PubMedCrossRefGoogle Scholar
  93. Muller G, Baigent CL (1980) Antigen-controlled immunodiagnosis- “ACID test”. J Immunol Methods 37: 185–190PubMedCrossRefGoogle Scholar
  94. Musher DM, Verbrugh HA, Verhoef J (1981) Suppression of phagocytosis and chemotaxis by cell wall components of Staphylococcus aureus. J Immunol 127: 84–88PubMedGoogle Scholar
  95. Nardiello S, Schaffner F, Vernace S, Paronetto F (1982) Pokeweed mitogen-induced immunoglobulin secreting cells in hepatitis B surface antigen-positive-negative chronic active hepatitis: evaluation by a protein A plaque assay. Clin Immunol Immunopathol 22: 168–179PubMedCrossRefGoogle Scholar
  96. Norrild B, Pedersen B, Roizman B (1981) Immunological reactivity of herpes simplex virus 1 and 2 polypeptides electrophoretically separated and transferred to diazobenzyloxymethyl paper. Infect Immun 31: 660–667PubMedGoogle Scholar
  97. Notani GW, Parsons JA, Erlandsen SL (1979) Versatility of Staphylococcus aureus protein A in immunocytochemistry. J Histochem Cytochem 27: 1438–1444PubMedCrossRefGoogle Scholar
  98. Nowinski RC, Lostrom ME, Tam MR, Stone MR, Burnette WN (1979) The isolation of hybrid cell lines producing monoclonal antibodies against the pl5(E) protein of ecotropic murine leukemia viruses. Virology 93: 111–126PubMedCrossRefGoogle Scholar
  99. O’Keefe E, Bennett V (1980) Use of immunoglobulin-loaded protein A-bearing staphylococci as a primary solid-phase immunoadsorbent radioimmunoassay. J Biol Chem 255: 561–568PubMedGoogle Scholar
  100. Pain D, Surolia A (1979) Protein A-enzyme monoconjugate as a versatile tool for enzyme immunoassays. FEBS Lett 107: 73–76PubMedCrossRefGoogle Scholar
  101. Para MF, Baucke RB, Spear PG (1982a) Glycoprotein gE of herpes simplex virus type 1: effects of anti-gE on virion infectivity and on virus-induced Fc-binding receptors. J Virol 41: 129–136PubMedGoogle Scholar
  102. Para MF, Goldstein L, Spear PG (1982b) Similarities and differences in the Fc-binding glycoprotein (gE) of herpes simplex virus types 1 and 2 and tentative mapping of the viral gene for this glycoprotein. J Virol 41: 137–144PubMedGoogle Scholar
  103. Patrick CC, Virella G, Koistinen J, Fudenberg HH (1977) Differential binding of IgA proteins of different subclasses and allotypes to staphylococcal protein A. Z Immun Forsch 153: 466–469Google Scholar
  104. Potgieter LND, Rouse BT, Webb-Martin TA (1980) Enzyme-linked immunosorbent assay, using staphylococcal protein A for detecting virus antibodies. Am J Vet Res 41: 978–980PubMedGoogle Scholar
  105. Pringle CR, Parry JE (1980) Location and quantitation of antigen on the surface of virus-infected cells by specific bacterial adherence and scanning electron microscopy. J Virol Methods 1: 61–75CrossRefGoogle Scholar
  106. Rahman AA, Teschner M, Sethi KK (1976) Appearance of IgG (Fc) receptor(s) on cultured human fibroblasts infected with human cytomegalovirus. J Immunol 117: 253–258PubMedGoogle Scholar
  107. Reiner M, Wecker E (1981) A modified absorption-reduction method to detect virus-specific hemagglutination inhibiting and neutralizing IgM antibodies. Med Microbiol Immunol 169: 237–245PubMedCrossRefGoogle Scholar
  108. Reisberg MA, Rossen RD (1981) Advantages and limitations of staphylococcal protein A-Sepharose for isolating soluble immune complexes from goat, rabbit and human sera. Clin Exp Immunol 46: 443–452PubMedGoogle Scholar
  109. Reiser J, Wardale J (1981) Immunological detection of specific proteins in total cell extracts by fractionation in gels and transfer to diazophenylthioether paper. Eur J Biochem 114:569– 575PubMedCrossRefGoogle Scholar
  110. Richman DD, Cleveland PH, Oxman MN, Zaia JA (1981a) A rapid radioimmunoassay using 125I-labeled staphylococcal protein A for antibody to varicella-zoster virus. J Infect Dis 143: 693–699PubMedCrossRefGoogle Scholar
  111. Richman DD, Cleveland P, Oxman M, van Wyke K, Webster R (1981b) Rapid characterization of influenza viruses using monoclonal antibodies (Abstr). 5th Intl Cong Virology, Strasbourg p 170Google Scholar
  112. Richman DD, Cleveland PH, Oxman MN, Johnson KM (1982) The binding of staphylococcal protein A by the sera of different animal species. J Immunol 128: 2300PubMedGoogle Scholar
  113. Richman DD, Cleveland PH, Oxman MN (to be published) A rapid enzyme immunofiltration technique using monoclonal antibodies to serotype herpes simplex virus. J Med VirolGoogle Scholar
  114. Richman DD, Cleveland PH, McCormick JB, Johnson KM (1983) Antigenic analysis of strains of Marburg and Ebola viruses: identification of two Ebola virus serotypes. J Infect Dis 147: 268–271PubMedCrossRefGoogle Scholar
  115. Roth J, Bendayan M, Orci L (1978) Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem 26: 163–169PubMedCrossRefGoogle Scholar
  116. Sakuma S, Furukawa T, Plotkin SA (1977) The characterization of IgG receptor induced by human cytomegalovirus. Proc Soc Exp Biol Med 155: 168–172PubMedGoogle Scholar
  117. Saltvedt E, Harboe M (1976) Binding of IgA to protein A-containing staphylococci: relationship to subclasses. Scand J Immunol 5: 1103–1108PubMedCrossRefGoogle Scholar
  118. Sandor M, Langone JJ (1981) Immune aggregation and not antigen-induced conformational change accounts for enhanced reactivity between immunoglobulin G and protein A. Biochem Biophys Res Comm 100: 1326–1331PubMedCrossRefGoogle Scholar
  119. Sjodahl J (1977) Repetitive sequences in protein A from Staphylococcus aureus. Arrangement of five regions within the protein, four being highly homologous and Fc-binding. Eur J Biochem 73: 343–351PubMedCrossRefGoogle Scholar
  120. Sjoholm I (1975) Protein A from Staphylococcus aureus. Spectropolarimetric and spectrophotometry studies. Eur J Biochem 51: 55–61PubMedCrossRefGoogle Scholar
  121. Sjoholm I, Bjerken A, Sjoquist J (1973) Protein A from Staphylococcus aureus. XIV. The effect of nitration of protein A with tetranitromethane and subsequent reduction. J Immunol 110: 1562–1569PubMedGoogle Scholar
  122. Sjoquist J, Stalenheim G (1969) Protein A from Staphylococcus aueus. IX. Complement-fixing activity of protein A-IgG complexes. J Immunol 103: 467–473PubMedGoogle Scholar
  123. Sjoquist J, Meloun B, Hjelm H (1972a) Protein A isolated from Staphylococcus aureus after digestion with lysostaphin. Eur J Biochem 29: 572–578PubMedCrossRefGoogle Scholar
  124. Sjoquist J, Movitz J, Johansson IB, Hjelm H (1972b) Localization of protein A in the bacteria. Eur J Biochem 30: 190–194PubMedCrossRefGoogle Scholar
  125. Skaug K, Gaarder PI (1978) An indirect immunofluorescent antibody test for determination of rubella virus–specific IgM antibodies. Elimination of secondary IgM rheumatoid factor staining after absorption of serum IgG with staphylococcal protein A. Acta Pathol Microbiol Scand [C] 86: 33–35Google Scholar
  126. Slot JW, Geuze HJ (1981) Sizing of protein A-colloidal gold globes for immunoelectron microscopy. J Cell Biol 90: 533–536PubMedCrossRefGoogle Scholar
  127. Soergel ME, Schaffer FL, Sawyer JC, Prato CM (1978) Assay of antibodies to caliciviruses by radioimmune precipitation using staphylococcal protein A as IgG adsorbent. Arch Virol 57: 271–282PubMedCrossRefGoogle Scholar
  128. Stalenheim G, Gotze O, Cooper NR, Sjoquist J, Muller-Eberhard HJ (1973) Consumption of human complement components by complexes of IgG with protein A of Staphylococcus aureus. Immunochemistry 10: 501–507PubMedCrossRefGoogle Scholar
  129. Stewart GA, Varro R, Stanworth DR (1978) The influence of enzymatic cleavage and chemical modification of human and rabbit IgG on their reactivity with staphylococcal protein A. Immunology 35: 785–791PubMedGoogle Scholar
  130. Surolia A, Pain D (1981) Preparation of protein A-enzyme monoconjugate and its use as a reagent in enzyme immunoassays. Methods Enzymol 73: 176–191CrossRefGoogle Scholar
  131. Tuomanen EI, Powell KR (1980) Staphylococcal protein A adsorption of neonatal serum to facilitate early diagnosis of congenital infection. J Pediatr 97: 238–243PubMedCrossRefGoogle Scholar
  132. Verhoef J, Peterson PK, Verbrugh HA (1979) Host-parasite relationship in staphylococcal infections. The role of the staphylococcal cell wall during the process of phagocytosis. Antonie Van Leeuwenhoek 45: 49–53PubMedCrossRefGoogle Scholar
  133. Verwey WF (1940) A type-specific antigenic protein derived from the Staphylococcus. J Exp Med 71. 635–644PubMedCrossRefGoogle Scholar
  134. Wilder RL, Yuen CC, Subbarao B, Woods VL, Alexander CB, Mage RG (1979) Tritium (3H) radiolabeling of protein A and antibody to high specific activity: application to cell surface antigen radioimmunoassays. J Immunol Methods 28: 255–266PubMedCrossRefGoogle Scholar
  135. Winblad S, Ericson C (1973) Sensitized sheep red cells as a reactant for Staphylococcus aureus protein A. Methodology and epidemiology with special reference to weakly reacting methicillin-resistant strains. Acta Pathol Microbiol Scand [B] 81: 150–156Google Scholar
  136. Yasuda J, Milgrom F (1968) Hemadsorption by herpes simplex-infected cell cultures. Int Arch Allergy Appl Immunol 33: 151–170PubMedCrossRefGoogle Scholar
  137. Yolken RH, Leister FJ (1981) Staphylococcal protein A-enzyme immunoglobulin conjugates: versatile tools for enzyme immunoassays. J Immunol Methods 43: 209–218PubMedCrossRefGoogle Scholar
  138. Yolken RH, Stopa PJ (1980) Comparison of seven enzyme immunoassay systems for measurement of cytomegalovirus. J Clin Microbiol 11: 546–551PubMedGoogle Scholar
  139. Zaia JA, Oxman MN (1977) Antibody to varicella-zoster virus-induced membrane antigen: im¬munofluorescence assay using monodisperse glutaraldehyde-fixed target cells. J Infect Dis 136: 519–530PubMedCrossRefGoogle Scholar
  140. Zeltzer PM, Pepose JS, Bishop NH, Miller JN (1978) Microassay for immunoglobulin G antibodies to Treponema pallidum with radio io dinated protein A from Staphylococcus aureus: immunoglobulin G response in experimental syphilis in rabbits. Infect Immun 21: 163–170PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1983

Authors and Affiliations

  • Douglas D. Richman
    • 1
  1. 1.Departments of Pathology and MedicineVeterans Administration Medical Center and University of CaliforniaSan DiegoUSA

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