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Phytochrome as a Molecule

  • Chapter
Book cover Photomorphogenesis

Part of the book series: Encyclopedia of Plant Physiology ((PLANT,volume 16))

Abstract

Action spectra studies of photomorphogenic responses in plants imply that several photoreceptor molecules probably exist (see Chap. 2, this Vol.). A necessary part of gaining an understanding of the mechanisms of these light-mediated responses in plants is the identification and characterization of the photoreceptor molecules. To date, the only photomorphogenically active molecule to be unequivocally identified and isolated from plants is phytochrome. The detection and isolation of phytochrome (Butler et al. 1959) was made possible by its unique photoreversible absorbance changes in the red and far-red regions of the spectrum which matched action spectra for photoreversible physiological responses in plants (Borthwick et al. 1952). These reversible spectral changes in phytochrome provided the basis for a photometric assay (Chap. 8, this Vol.) and made possible the subsequent purification and characterization of phytochrome.

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References

  • Anderson GR, Jenner EL, Mumford FE (1970) Optical rotatory dispersion and circular dichroism spectra of phytochrome. Biochim Biophys Acta 221:69–73

    Article  CAS  PubMed  Google Scholar 

  • Balangé AP, Rollin P (1973) Purification of photoreversible phytochrome from Avena seedlings by isoelectric focusing. Plant Sci Lett 1:59–64

    Article  Google Scholar 

  • Bolton GW, Quail PH (1981a) Cell-free synthesis of Avena phytochrome. Plant Physiol 67 (suppl):130

    Google Scholar 

  • Bolton GW, Quail PH (1981b) A method for preparing green plant tissue extracts for spectrophotometric measurement of phytochrome. Plant Physiol 67 (suppl): 587

    Google Scholar 

  • Bolton GW, Quail PH (1982) Cell-free synthesis of phytochrome apoprotein. Planta 155:212–217

    Article  CAS  Google Scholar 

  • Borthwick HA, Hendricks SB, Parker MW, Toole EH, Toole VK (1952) A reversible photoreaction controlling seed germination. Proc Natl Acad Sci USA 38:662–666

    Article  CAS  PubMed  Google Scholar 

  • Braslavsky SE, Matthews JI, Herbert HJ, DeKok J, Spruit CJP, Schaffner K (1980) Characterization of a microsecond intermediate in the laser flash photolysis of small phytochrome from oat. Photochem Photobiol 31:417–420

    Article  CAS  Google Scholar 

  • Briggs WR, Fork DC (1969a) Long-lived intermediates in phytochrome transformation I: in vitro studies. Plant Physiol 44:1081–1088

    Article  CAS  PubMed  Google Scholar 

  • Briggs WR, Fork DC (1969b) Long-lived intermediates in phytochrome transformation II: in vitro and in vivo studies. Plant Physiol 44:1089–1094

    Article  CAS  PubMed  Google Scholar 

  • Briggs WR, Rice HV (1972) Phytochrome: chemical and physical properties and mechanism of action. Annu Rev Plant Physiol 23:293–334

    Article  CAS  Google Scholar 

  • Briggs WR, Zollinger WD, Platz BB (1968) Some properties of phytochrome isolated from dark-grown oat seedlings (Avena saliva L.). Plant Physiol 43:1239–1243

    Article  CAS  PubMed  Google Scholar 

  • Briggs WR, Gardner G, Hopkins DW (1972) Some technical problems in the purification of phytochrome. In: Mitrakos K, Shropshire W (eds) Phytochrome. Academic Press, London New York, pp 145–158

    Google Scholar 

  • Butler WL (1961) Some photochemical properties of phytochrome. In: Christensen B, Buchmann B (eds) Progress in photobiology. Elsevier, Amsterdam, pp 569–571

    Google Scholar 

  • Butler WL, Norris KH, Siegelman HW, Hendricks SB (1959) Detection, assay, and preliminary purification of the pigment controlling photoresponsive development of plants. Proc Natl Acad Sci USA 45:1703–1708

    Article  CAS  PubMed  Google Scholar 

  • Butler WL, Lane HC, Siegelman HW (1963) Nonphotochemical transformation of phytochrome in vivo. Plant Physiol 38:514–519

    Article  CAS  PubMed  Google Scholar 

  • Butler WL, Hendricks SB, Siegelman HW (1964a) Action spectra of phytochrome in vitro. Photochem Photobiol 3:521–528

    Article  CAS  Google Scholar 

  • Butler WL, Siegelman HW, Miller CO (1964b) Denaturation of phytochrome. Biochemistry 3:851–857

    Article  CAS  PubMed  Google Scholar 

  • Capaldi RA, Vanderkooi G (1972) The low polarity of many membrane proteins. Proc Natl Acad Sci USA 69:930–932

    Article  CAS  PubMed  Google Scholar 

  • Cecil R (1963) Role of sulfur in proteins. In: Neurath H (ed) The proteins, 2nd edn, Vol 1. Academic Press, New York, pp 379–476

    Google Scholar 

  • Clarkson DT, Hillman WS (1968) Stable concentrations of phytochrome in Pisum under continuous illumination with red light. Plant Physiol 43:88–92

    Article  CAS  PubMed  Google Scholar 

  • Cordonnier M-M, Pratt LH (1980) Preparation, characterization, and utilization of antiserum against zucchini phytochrome. In: DeGreef J (ed) Photoreceptors and plant development, Antwerpen Univ Press, Antwerpen, pp 69–78

    Google Scholar 

  • Cordonnier M-M, Pratt LH (1982) Immunopurification and initial characterization of dicotyledonous phytochrome. Plant Physiol 69:360–365

    Article  CAS  PubMed  Google Scholar 

  • Correli DL, Edwards JL, Klein WH, Shropshire W (1968a) Phytochrome in etiolated annual rye. III. Isolation of photoreversible phytochrome. Biochim Biophys Acta 168:36–45

    Article  Google Scholar 

  • Correli DL, Steers E, Towe KM, Shropshire W (1968b) Phytochrome in etiolated annual rye. IV. Physical and chemical characterization of phytochrome. Biochim Biophys Acta 168:46–57

    Article  Google Scholar 

  • Correli DL, Edwards JL, Shropshire W (1968c) Multiple chromophore species in phytochrome. Photochem Photobiol 8:465–475

    Article  Google Scholar 

  • Cross DR, Linschitz H, Kasche V, Tenenbaum J (1968) Low-temperature studies on phytochrome : light and dark reactions in the red to far-red transformation and new intermediate forms of phytochrome. Proc Natl Acad Sci USA 61:1095–1101

    Article  CAS  PubMed  Google Scholar 

  • Cundiff SC, Pratt LH (1975) Phytochrome characterization by rabbit antiserum against high molecular weight phytochrome. Plant Physiol 55:207–211

    Article  CAS  PubMed  Google Scholar 

  • Frankland B (1972) Biosynthesis and dark transformations of phytochrome. In: Mitrakos K, Shropshire W (eds) Phytochrome. Academic Press, London New York, pp 195–225

    Google Scholar 

  • Fry KT, Mumford FE (1971) Isolation and partial characterization of a chromophore- peptide fragment from pepsin digests of phytochrome. Biochem Biophys Res Commun 45:1466–1473

    Article  CAS  PubMed  Google Scholar 

  • Gardner G, Briggs WR (1974) Some properties of phototransformation of rye phytochrome in vitro. Photochem Photobiol 19:367–377

    Article  CAS  PubMed  Google Scholar 

  • Gardner G, Pike CS, Rice HV, Briggs WR (1971) “Disaggregation” of phytochrome in vitro - a consequence of proteolysis. Plant Physiol 48:686–693

    Article  CAS  PubMed  Google Scholar 

  • Gardner G, Thompson WF, Briggs WR (1974) Differential reactivity of the red- and far-red-absorbing forms of phytochrome to [14C]N-ethyl maleimide. Planta 117:367–372

    Article  CAS  Google Scholar 

  • Grombein S, Rüdiger W (1976) On the molecular weight of phytochrome: a new high molecular phytochrome species in oat seedlings. Hoppe-Seyler’s Z Physiol Chem 357:1015–1018

    CAS  PubMed  Google Scholar 

  • Hahn T-R, Song P-S (1981) Hydrophobic properties of phytochrome as probed by 8-ani- linonaphthalene-1-sulfonate fluorescence. Biochemistry 20 :2602–2609

    Article  CAS  PubMed  Google Scholar 

  • Hahn T-R, Kang S-S, Song P-S (1980) Difference in the degree of exposure of chromo- phores in the Pr and Pfr forms of phytochrome. Biochem Biophys Res Commun 97:1317–1323

    Article  CAS  PubMed  Google Scholar 

  • Heim B, Jabben M, Schäfer E (1981) Phytochrome destruction in dark- and light-grown Amaranthus caudatus seedlings. Photochem Photobiol 34:89–93

    CAS  Google Scholar 

  • Hopkins DW (1971) Protein conformational changes of phytochrome. PhD dissertation, Univ California, San Diego

    Google Scholar 

  • Hopkins DW, Butler WL (1970) Immunochemical and spectroscopic evidence for protein conformational changes in phytochrome transformations. Plant Physiol 45:567–570

    Article  CAS  PubMed  Google Scholar 

  • Hunt RE, Pratt LH (1979) Phytochrome immunoaffmity purification. Plant Physiol 64:332–336

    Article  CAS  PubMed  Google Scholar 

  • Hunt RE, Pratt LH (1980) Partial characterization of undegraded oat phytochrome. Biochemistry 19:390–394

    Article  CAS  PubMed  Google Scholar 

  • Hunt RE, Pratt LH (1981) Physicochemical differences between the red- and the far-red- absorbing forms of phytochrome. Biochemistry 20:941–945

    Article  CAS  PubMed  Google Scholar 

  • Kendrick RE (1974) Phytochrome intermediates in freeze-dried tissue. Nature 250:159–161

    Article  CAS  Google Scholar 

  • Kendrick RE, Spruit CJP (1977) Phototransformations of phytochrome. Photochem Photobiol 26:201–214

    Article  CAS  PubMed  Google Scholar 

  • Kim I-S, Song P-S (1981) Binding of phytochrpme to liposomes and protoplasts. Biochemistry 20:5482–5489

    Article  CAS  PubMed  Google Scholar 

  • Lagarias JC, Rapaport H (1980) Chromopeptides from phytochrome. The structure and linkage of the Pr form of the phytochrome chromophore. J Am Chem Soc 102:4821–4828

    Article  CAS  Google Scholar 

  • Linschitz H, Kasche Y (1967) Kinetics of phytochrome conversion: multiple pathways in the Pr to Pfr reaction, as studied by double-flash technique. Proc Natl Acad Sci USA 58:1059–1064

    Article  CAS  PubMed  Google Scholar 

  • Linschitz H, Kasche V, Butler WL, Siegelman HW (1966) The kinetics of phytochrome conversion. J Biol Chem 241:3395–3403

    CAS  PubMed  Google Scholar 

  • Lisansky SG, Galston AW (1974) Phytochrome stability in vitro. I. Effect of metal ions. Plant Physiol 53:352–359

    Article  CAS  PubMed  Google Scholar 

  • Litts JC (1980) Phytochrome: A purification and partial characterization. PhD dissertation, Univ Minnesota

    Google Scholar 

  • Mumford FE, Jenner EL (1966) Purification and characterization of phytochrome from oat seedlings. Biochemistry 5:3657–3662

    Article  CAS  PubMed  Google Scholar 

  • Negbi M, Hopkins DW, Briggs WR (1975) Acceleration of dark reversion of phytochrome in vitro by calcium and magnesium. Plant Physiol 56:157–159

    Article  CAS  PubMed  Google Scholar 

  • Oelze-Karow H, Schäfer E, Mohr H (1976) On the physiological significance of dark reversion of phytochrome in the mustard seedling. Photochem Photobiol 23:55–59

    Article  CAS  PubMed  Google Scholar 

  • Pike CS, Briggs WR (1972) The dark reactions of rye phytochrome in vivo and in vitro. Plant Physiol 49:514–520

    Article  CAS  PubMed  Google Scholar 

  • Pratt LH (1973) Comparative immunochemistry of phytochrome. Plant Physiol 51:203–209

    Article  CAS  PubMed  Google Scholar 

  • Pratt LH (1975) Kinetic analysis of a very rapidly reverting population of high-molecular- weight phytochrome. Photochem Photobiol 21:99–103

    Article  CAS  PubMed  Google Scholar 

  • Pratt LH (1978) Molecular properties of phytochrome. Photochem Photobiol 27:81–105

    Article  CAS  Google Scholar 

  • Pratt LH (1979) Phytochrome: functions and properties. Photochem Photobiol Rev 4:59–124

    Article  CAS  Google Scholar 

  • Pratt LH (1982a) Phytochrome purification. In: Smith H (ed) Techniques in photomorphogenesis. Academic Press, London New York (in press)

    Google Scholar 

  • Pratt LH (1982b) Phytochrome: the protein moity. Annu Rev Plant Physiol 33:557–582

    Article  CAS  Google Scholar 

  • Pratt LH, Butler WL (1968) Stabilization of phytochrome intermediates by low temperature. Photochem Photobiol 8:477–485

    Article  CAS  PubMed  Google Scholar 

  • Pratt LH, Butler WL (1970) The temperature dependence of phytochrome transformations. Photochem Photobiol 11:361–369

    Article  CAS  PubMed  Google Scholar 

  • Pratt LH, Cundiff SC (1975) Spectral characterization of high-molecular-weight phytochrome. Photochem Photobiol 21:91–97

    Article  CAS  PubMed  Google Scholar 

  • Pratt LH, Kidd GH, Coleman RA (1974) An immunochemical characterization of the phytochrome destruction reaction. Biochim Biophys Acta 365:93–107

    Article  CAS  PubMed  Google Scholar 

  • Quail PH, Schäfer E, Marmé D (1973a) De novo synthesis of phytochrome in pumpkin hooks. Plant Physiol 52:124–127

    Article  CAS  PubMed  Google Scholar 

  • Quail PH, Schäfer E, Marmé D (1973b) Turnover of phytochrome in pumpkin cotyledons. Plant Physiol 52:128–131

    Article  CAS  PubMed  Google Scholar 

  • Rice HV, Briggs WR (1973a) Partial characterization of oat and rye phytochrome. Plant Physiol 51:927–938

    Article  CAS  PubMed  Google Scholar 

  • Rice HV, Briggs WR (1973b) Immunochemistry of phytochrome. Plant Physiol 51:939–945

    Article  CAS  PubMed  Google Scholar 

  • Rice HV, Briggs WR, Jackson-White CJ (1973) Purification of oat and rye phytochrome. Plant Physiol 51:917–926

    Article  CAS  PubMed  Google Scholar 

  • Roux SJ (1972) Chemical evidence for conformational differences between the red- and far-red-absorbing forms of oat phytochrome. Biochemistry 11:1930–1936

    Article  CAS  PubMed  Google Scholar 

  • Roux SJ, Linsansky SG, Stoker BM (1975) Purification and partial carbohydrate analysis of phytochrome from Avena sativa. Physiol Plant 35:85–90

    Article  CAS  Google Scholar 

  • Roux SJ, McEntire K, Brown WE (1982) Determination of extinction coefficients of oat phytochrome by quantitative amino acid analyses. Photochem Photobiol 35:537–543

    Article  CAS  Google Scholar 

  • Rüdiger W (1980) Phytochrome, a light receptor or plant photomorphogenesis. Struct Bonding 40:101–140

    Article  Google Scholar 

  • Sarkar HK, Song P-S (1982) Blue light induced photo transformation of phytochrome in the presence of flavin. Photochem Photobiol 35:243–246

    Article  CAS  Google Scholar 

  • Shimazaki Y, Inoue Y, Yamamoto KT, Furuya M (1981) Photo transformation of the red-light-absorbing form of undegraded pea phytochrome by laser flash excitation. Plant Cell Physiol 21:1619–1625

    Google Scholar 

  • Siegelman HW, Firer EM (1964) Purification of phytochrome from oat seedlings. Biochemistry 3:418–423

    Article  CAS  PubMed  Google Scholar 

  • Smith H (1975) Phytochrome and phötomorphogenesis. McGraw-Hill, London

    Google Scholar 

  • Smith WO (1975) Purification and physicochemical studies of phytochrome. PhD Dissertation, Univ Kentucky

    Google Scholar 

  • Smith WO (1978) The effects of neutral salts on the dark reversion of phytochrome. Plant Physiol 61 (Suppl): 61

    Google Scholar 

  • Smith WO (1981) Probing the molecular structure of phytochrome with immobilized Cibacron blue 3GA and blue dextran. Proc Natl Acad Sci USA 78:2977–2980

    Article  CAS  PubMed  Google Scholar 

  • Smith WÖ, Correli DL (1975) Phytochrome: a re-examination of the quaternary structure. Plant Physiol 56:340–343

    Article  CAS  PubMed  Google Scholar 

  • Smith WO Daniels SM (1981) Purification of phytochrome by affinity chromatography on agarose-immobilized Cibacron blue 3GA. Plant Physiol 68:443–446

    Article  CAS  PubMed  Google Scholar 

  • Song P-S, Chae Q, Gardner JD (1979) Spectroscopic properties and chromophore conformations of the photomorphogenic receptor: phytochrome. Biochim Biophys Acta 576:479–495

    Article  CAS  PubMed  Google Scholar 

  • Song P-S, Kim I-S, Hahn T-R (1981) Purification of phytochrome by affi-gel blue chromatography; an effect of lumichrome on purified phytochrome. Anal Biochem 117:32–39

    Article  CAS  PubMed  Google Scholar 

  • Stellwagen E (1977) Use of blue dextran as a probe for the nicotinamide adenine dinucleotide domain in proteins. Acc Chem Res 10:92–98

    Article  CAS  Google Scholar 

  • Stoker BM, Roux SJ, Brown WE (1978) Evidence for symmetry in the phytochrome subunit. Nature 271:180–182

    Article  CAS  Google Scholar 

  • Stone HJ, Pratt LH (1979) Characterization of the destruction of phytochrome in the red-absorbing form. Plant Physiol 63:680–682

    Article  CAS  PubMed  Google Scholar 

  • Taylor AO, Bonner BA (1967) Isolation of phytochrome from the alga Mesotaenium and liverwort Sphaerocarpus. Plant Physiol 42:762–766

    Article  CAS  PubMed  Google Scholar 

  • Tobin EM, Briggs WR (1973) Studies on the protein conformation of phytochrome. Photochem Photobiol 18 : 487–495

    Article  CAS  PubMed  Google Scholar 

  • Tokutomi S, Yamamoto KT, Furuya M (1981) Photoreversible changes in hydrophobi- city of undegraded pea phytochrome determined by partition in an aqueous two-phase system. FEBS Lett 134:159–162

    Article  CAS  Google Scholar 

  • Yamamoto KT, Smith WO (1981a) Alkyl and ω-amino alkyl agaroses as probes of light-induced changes in phytochrome from pea seedlings (Pisum sativum cv. Alaska). Biochim Biophys Acta 668:27–34

    Article  CAS  PubMed  Google Scholar 

  • Yamamoto KT, Smith WO (1981b) Effect of neutral salts on spectral characteristics of undegraded phytochrome partially purified from etiolated pea shoots. Plant Cell Physiol 22:1149–1158

    CAS  Google Scholar 

  • Yamamoto KT, Smith WO (1981c) A re-evaluation of the mole fraction of Pfr at the red-light-induced photo stationary state of undegraded rye phytochrome. Plant Cell Physiol 22:1159–1164

    CAS  Google Scholar 

  • Yamamoto KT, Smith WO, Furuya M (1980) Photoreversible Ca2+-dependent aggregation of purified phytochrome from etiolated pea and rye seedlings. Photochem Photobiol 32:233–239

    Article  CAS  Google Scholar 

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© 1983 Springer-Verlag Berlin Heidelberg

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Smith, W.O. (1983). Phytochrome as a Molecule. In: Shropshire, W., Mohr, H. (eds) Photomorphogenesis. Encyclopedia of Plant Physiology, vol 16. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68918-5_6

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  • DOI: https://doi.org/10.1007/978-3-642-68918-5_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-68920-8

  • Online ISBN: 978-3-642-68918-5

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